Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses

Bharathi, Jothi Kanmani; Anandan, R.; Benjamin, Lincy Kirubhadharsini; Muneer, Sowbiya; Samy, Prakash Muthu Arjuna

doi:10.1016/j.plaphy.2022.11.035

Cited by 29 publications

(16 citation statements)

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“…Development, epigenetic changes, and biotic and abiotic stresses all include the RNA silencing mechanism, which is mediated by the three primary RNAi proteins DCL, AGO, and RDR [14,15,106]. The 19-24 nucleotide single-stranded non-coding RNA molecules known as microRNAs (miRNAs), which have undergone evolutionary conservation, control the expression of certain genes or gene clusters at the TGS and PTGS levels [19,[89][90][91]107].…”

Section: Regulatory Network With Micro-rnas (Mirnas)mentioning

confidence: 99%

“…MicroRNA (miRNA) and short interfering RNA (siRNA) are two small RNAs (sRNAs) with a length of 21-24 nucleotides that are involved in both TGS and PTGS. These sRNAs assist plants in building up their natural defenses and protecting themselves against stresses [13][14][15]. The DCL, AGO, and RDR genes produce the RNAi (DCL, AGO, and RDR) proteins, which are essential for the biosynthesis and activity of sRNAs [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and characterization of major RNAi genes highlighting their regulatory factors in Cowpea (Vigna unguiculata(L.) Walp.)

Hasan

Mosharaf

Uddin

et al. 2023

Preprint

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In different regions of the world, cowpea (Vigna unguiculata (L.) Walp.) is an important vegetable and an excellent source of protein. It lessens the malnutrition of the underprivileged in developing nations and has some positive effects on health, such as a reduction in the prevalence of cancer and cardiovascular disease. However, occasionally, certain biotic and abiotic stresses caused a sharp fall in cowpea yield. Currently, short RNAs (sRNAs) are thought to play a crucial role in controlling how stress-related genes are expressed in plants as they go through various developmental phases. Major RNA interference (RNAi) genes like Dicer-like (DCL), Argonaute (AGO), and RNA-dependent RNA polymerase (RDR) are essential for sRNA synthesis processes and shield plants from biotic and abiotic stresses. In this study, applying BLASTP search and phylogenetic tree analysis with reference to the Arabidopsis RNAi (AtRNAi) genes, we discovered 28 VuRNAi genes, including 7 VuDCL, 14 VuAGO, and 7 VuRDR genes in cowpea. We looked at the domains, motifs, gene structures, chromosomal locations, sub-cellular locations, gene ontology (GO) terms and regulatory factors (transcription factors, micro-RNAs, and cis-acting factors) to characterize the VuRNAi genes and proteins in cowpea. Predicted VuDCL1, VuDCL2(a, b), VuAGO7, VuAGO10 and VuRDR6 genes might have an impact on cowpea growth, development of the vegetative and flowering stages and antiviral defense. The VuRNAi gene regulatory features miR395 and miR396 might contribute to grain quality improvement, immunity boosting, and pathogen infection resistance under salinity and drought conditions. Predicted cis-acting elements (CAEs) from the VuRNAi gene associated with light responsiveness (LR), stress responsiveness (SR) and hormone responsiveness (HR) might play a role in plant growth and development, improving grain quality and production and protecting plants from biotic and abiotic stresses. Therefore, our study provides crucial information about the functional roles of VuRNAi genes and their regulatory components, which would aid in the development of future cowpeas that are more resilient to biotic and abiotic stress.

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Section: Regulatory Network With Micro-rnas (Mirnas)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of major RNAi genes highlighting their regulatory factors in Cowpea (Vigna unguiculata(L.) Walp.)

Hasan

Mosharaf

Uddin

et al. 2023

Preprint

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“…Its discovery in Caenorhabditis elegans demonstrated the ability to silence target genes upon the introduction of dsRNA with the same sequences (Fire et al, 1998). With the advent of this technology, its application has also been widely carried out in many other species such as insects (Cooper et al, 2019; Gu and Knipple, 2013; Kong et al, 2014), plants (Bharathi et al, 2023; Zotti et al, 2018), and mammals (Carmell et al, 2003; Song et al, 2003). By regulating the expression of relevant genes, it is of great significance in the study of gene function, yield improvement, and disease prevention.…”

Section: Introductionmentioning

confidence: 99%

Non-Invasive dsRNA Delivery via Feeding for Effective Gene Silencing in Teleost Fish: A Novel Approach in the Study of Gene Function Analysis

Gao,

Yang,

Song

et al. 2024

Preprint

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RNA interference is a powerful technique for gene silencing, involving post-transcriptional regulation of gene expression. Successful application of RNAi has been demonstrated in various organisms like nematodes, insects, and oysters by ingesting dsRNA-expressing bacteria. In this study, we attempted a non-invasive feeding method in the marine economy teleost fish,Sebastes schlegelii. To validate the feasibility of this approach inS. schlegelii, we utilized rotifers (Brachionus plicatilis),Artemia nauplii, and commercial pellet feed as vectors to deliverEscherichia colistrain HT115 engineered to express double-stranded RNA (dsRNA), targetingdead end(dnd) gene, known to be important for primordial germ cells (PGCs) migration and proliferation in fish. Our experimental results consistently showed that knockdown of thedndgene effectively inhibited the development of PGCs inS. schlegelii, resulting in gonadal dysplasia and even sex reversal. This study marks a significant advancement in understanding gene function in teleost fish, laying the groundwork for future large-scale research in this field.

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“…One strand of the siRNA duplex is incorporated into the Argonaute protein (AGO), forming the RNA-induced silencing complex (RISC). The siRNA molecule directs RISC to the complementary region of the single-stranded RNA, after which AGO cleaves the target mRNA (Zhuravlyov et al, 2022;Bharathi et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene

Elkonin,

Borisenko,

Pylaev

et al. 2024

Vestn. VOGiS

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Improving the nutritional value of grain sorghum, a drought- and heat-tolerant grain crop, is an important task in the context of global warming. One of the reasons for the low nutritional value of sorghum grain is the resistance of its storage proteins (kafirins) to proteolytic digestion, which is due, among other things, to the structural organization of protein bodies, in which γ-kafirin, the most resistant to proteases, is located on the periphery, encapsulating more easily digested α-kafirins. The introduction of genetic constructs capable of inducing RNA silencing of the γ-kafirin (gKAF1) gene opens up prospects for solving this problem. Using Agrobacterium-mediated genetic transformation of immature embryos of the grain sorghum cv. Avans we have obtained a mutant with improved digestibility of endosperm proteins (up to 92 %) carrying a genetic construct for RNA silencing of the gKAF1 gene. The goal of this work was to study the stability of inheritance of the introduced genetic construct in T2–T4 generations, to identify the number of its copies, as well as to trace the manifestation of agronomically valuable traits in the offspring of the mutant. The mutant lines were grown in experimental plots in three randomized blocks. The studied lines were characterized by improved digestibility of kafirins, a modified type of endosperm, completely or partially devoid of the vitreous layer, an increased percentage of lysine (by 75 %), reduced plant height, peduncle length, 1000-grains weight, and grain yield from the panicle. In T2, a line with monogenic control of GA resistance was selected. qPCR analysis showed that in different T3 and T4 plants, the genetic construct was present in 2–4 copies. In T3, a line with a high digestibility of endosperm proteins (81 %) and a minimal decrease in agronomically valuable traits (by 5–7 %) was selected.

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Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses

Cited by 29 publications

References 283 publications

Genome-wide identification and characterization of major RNAi genes highlighting their regulatory factors in Cowpea (Vigna unguiculata(L.) Walp.)

Genome-wide identification and characterization of major RNAi genes highlighting their regulatory factors in Cowpea (Vigna unguiculata(L.) Walp.)

Non-Invasive dsRNA Delivery via Feeding for Effective Gene Silencing in Teleost Fish: A Novel Approach in the Study of Gene Function Analysis

Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene

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