Recent Studies and Progression of Yin Chen Hao (茵陳蒿 Yīn Chén Hāo), a Long-term Used Traditional Chinese Medicine

Hung, Hsin Yi; Kuo, Sheng Chu

doi:10.4103/2225-4110.106533

Cited by 12 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scoparone is a natural coumarin derivative with many potential therapeutic effects [1][2][3][4][5][6][7][8][9][10]. It is therefore important to elucidate its pharmacokinetics, including metabolism, in humans and experimental animals.…”

Section: Discussionmentioning

confidence: 99%

Comparison of In Vitro Hepatic Scoparone 7-O-Demethylation between Humans and Experimental Animals

et al. 2017

View full text Add to dashboard Cite

Scoparone is a natural bioactive compound in Chinese herbal medicines. It has numerous pharmacological actions, including liver protective, hypolipidemic, antitumor, and anti-inflammatory effects. The primary metabolism route of scoparone is O-demethylation to scopoletin or isoscopoletin catalyzed by CYP enzymes. The aims of our study were to identify the human CYP enzymes catalyzing scoparone 7-O-demethylation to scopoletin and to compare this oxidation reaction in liver microsomes among different species. A high throughput fluorescent-based assay method was developed to determine the scoparone 7-O-demethylation to scopoletin rate. The rate was 100 - 400 nmol/(min×g protein) in mouse and rabbit liver microsomes, 10 - 20 nmol/(min×g protein) in pig microsomes, 1 - 3 nmol/(min×g protein) in human and less than 1 nmol/(min×g protein) in rat liver microsomes. Human CYP1A1 (K 13 µM and V 0.8 min), CYP1A2 (K 48 µM and V 0.3 min), and CYP2A13 (K 10 µM and V 22 min) were the most efficient catalysts of the reaction. The CYP2A6 selective inhibitor pilocarpine and an antibody against mouse CYP2A5 inhibited scoparone 7-O-demethylation to scopoletin in rabbit, mouse, and pig liver microsomes, indicating involvement of CYP2A enzymes in the reaction. Hepatic scoparone 7-O-demethylation to scopoletin differed between species both with respect to the rate of reaction and catalyzing enzymes. These species differences need to be taken into account when testing scoparone pharmacokinetics in animals and humans.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of In Vitro Hepatic Scoparone 7-O-Demethylation between Humans and Experimental Animals

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Scoparone is a bioactive coumarin derivative found in multiple plants. It is the major active component in the Chinese herbal medicine Yin Chen Hao, a component in many traditional Chinese medicine (TCM) products [ 52 ]. We developed a fluorescent-based assay method to evaluate the rate of scoparone 7-O-demethylation to scopoletin in various species [ 53 ].…”

Section: Use Of Coumarin Substrates In Studying Xmesmentioning

confidence: 99%

Coumarin-Based Profluorescent and Fluorescent Substrates for Determining Xenobiotic-Metabolizing Enzyme Activities In Vitro

Raunio

Pentikäinen

Juvonen

2020

IJMS

View full text Add to dashboard Cite

in vivo methods, such as spectrophotometric, fluorometric, mass spectrometric,and radioactivity-based techniques. In fluorescence-based assays, the reaction produces a fluorescentproduct from a nonfluorescent substrate or vice versa. Fluorescence-based enzyme assays areusually highly sensitive and specific, allowing measurements on small specimens of tissues withlow enzyme activities. Fluorescence assays are also amenable to miniaturization of the reactionmixtures and can thus be done in high throughput. 7-Hydroxycoumarin and its derivatives arewidely used as fluorophores due to their desirable photophysical properties. They possess a large -conjugated system with electron-rich and charge transfer properties. This conjugated structure leadsto applications of 7-hydroxycoumarins as fluorescent sensors for biological activities. We describe inthis review historical highlights and current use of coumarins and their derivatives in evaluatingactivities of the major types of xenobiotic-metabolizing enzyme systems. Traditionally, coumarinsubstrates have been used to measure oxidative activities of cytochrome P450 (CYP) enzymes. For thispurpose, profluorescent coumarins are very sensitive, but generally lack selectivity for individual CYPforms. With the aid of molecular modeling, we have recently described several new coumarin-basedsubstrates for measuring activities of CYP and conjugating enzymes with improved selectivity.

show abstract

“…Yin chen hao (Artemisiacapillaris Thunb), known as Yin Chen, Capillary or Oriental Wormwood, belonging to Asteracea family, is reported to have many beneficial effects and has been traditionally used to treat hepatic disorders (Reviewed by Hung and Kuo 2013;Lee et al 2007). Most research related to YCD and its protective effects on the liver were conducted with hamsters (Hong and Lee 2009) or rats (Wang et al 2012;Zhou et al 2013;Zhang et al 2011), while little is known regarding its effects on the liver of fish.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp (Cyprinus carpio var. Jian)

Zhou

Wang³

et al. 2014

Fish Physiol Biochem

View full text Add to dashboard Cite

In order to identify effective hepatoprotective herbs for clinical application in fish farming, 200 mg/kg olaquindox (OLA) was added to a basal diet (group 1, control) to form OLA diet (group 2), then 1.35, 2.7 and 5.4 % (w/w) of a Chinese herbal formulation, Yingchen decoction (YCD), were added to the OLA diet to form three additional diets for groups 3, 4 and 5, respectively. A total of 375 juvenile Jian carp (Cyprinus carpio var. Jian) (52.12 ± 2.95 g/tail) were divided into five groups (triplicates per group) and fed the five diets mentioned above, respectively, for 6 weeks. At the termination of feeding experiment, serum biochemical indexes, viability of hepatocytes and the hepatopancreas microstructure for each group were detected and observed. The results showed that serum ALT and AST in group 2 were significantly higher than the control (P < 0.05). Plasma membranes hepatocyte nuclei in group 2 were found to be mostly indistinct, compared to group 1, and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The viability of isolated hepatocytes in group 2 was the lowest and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The results suggest that YCD protected the Jian carp hepatopancreas against injury from OLA, and that 5.4 % YCD would be the optimum dosage in a Jian carp diet.

show abstract

Recent Studies and Progression of Yin Chen Hao (茵陳蒿 Yīn Chén Hāo), a Long-term Used Traditional Chinese Medicine

Abstract: Yin Chen Hao (Artemisia capillaris Thunb; 茵陳蒿 Yīn Chén Hāo) is a traditional Chinese medicine for treating hepatic disorders. This review provides recent pharmacological studies of Yin Chen Hao as well as some chemical constituents isolated from Yin Chen.

Cited by 12 publications

References 28 publications

Comparison of In Vitro Hepatic Scoparone 7-O-Demethylation between Humans and Experimental Animals

Comparison of In Vitro Hepatic Scoparone 7-O-Demethylation between Humans and Experimental Animals

Coumarin-Based Profluorescent and Fluorescent Substrates for Determining Xenobiotic-Metabolizing Enzyme Activities In Vitro

Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp (Cyprinus carpio var. Jian)

Contact Info

Product

Resources

About