“…Each of this component varies in different ratio from biomass to biomass. In general lignocellulose composes of cellulose, hemicellulose and lignin in the range of 40-50%, 20-30%, and 10-25%, respectively [4][5]. Cellulose is a polysaccharide chain of glucose units linked via β-1,4 glycosidic bonds.…”
Section: Introductionmentioning
confidence: 99%
“…[9][10][11] In recent years, several pretreatment methods have been developed for the fractionation of lignocellulosic biomass. Pretreatment methods include physical pretreatment (mechanical size reduction, pyrolysis), chemical pretreatment (ozonolysis, acid pretreatment, alkali pretreatment, oxidative delignification), physicochemical pretreatment (stream explosion, ammonia fiber explosion, microwave pretreatment, carbon dioxide explosion, wet oxidation) and biological pretreatment (fungus and bacteria) [4], [12], [13][14][15][16][17]. Pretreatment is an essential step prior to saccharification process.…”
Section: Introductionmentioning
confidence: 99%
“…After pretreatment, cellulose is converted to glucose by the cellulase enzymes due the ease in accessibility. Though pretreatment methods have advantages in the fractionation of biomass, each pretreatment method has its own disadvantage, for instance, some pretreatment methods require high energy or use toxic substances or have high operational cost [4].…”
Commonly, the agricultural waste, i.e. lignocellulosic biomass is disposed through combustion causing air pollution with production of PM2.5 and PM10 particles. However, it has been found that these biomasses can be used as source for the production of biofuels and other valuable biochemicals. Though deconstruction of lignocellulosic biomass is challenging due to its complex structure. In this study, rice straw (RS) was pretreated using potassium permanganate (KMnO4) to enhance the enzymatic saccharification efficiency. The study was carried out by varying the operational factors in pretreatment, including temperature (30-90°C), time (30-360 min) and concentration of KMnO4 (0.5-3.0, % w/v), respectively, based on Box-Behnken design (BBD). Through multi-regression analysis of the experimental data obtained after pretreatment, the optimum conditions were determined. The optimum conditions for temperature, time and potassium permanganate concentration were 48.09°C, 360 min, and 1.36% w/v, respectively. The saccharifications of pretreatment and untreated rice straw were carried out using Cellic Ctec2. The reducing sugar was determined by using DNS method and the yields of the untreated and pretreated RS were 32.38 and 49.011 mg/mL, respectively. The results showed that the sugar for pretreated RS were 1.51 fold times higher compared to untreated RS. Therefore, this work illustrates the pretreatment efficiency for KMnO4 to enhance the reducing sugar yield during saccharification, which can be used for biofuel and value-added product productions.
“…Each of this component varies in different ratio from biomass to biomass. In general lignocellulose composes of cellulose, hemicellulose and lignin in the range of 40-50%, 20-30%, and 10-25%, respectively [4][5]. Cellulose is a polysaccharide chain of glucose units linked via β-1,4 glycosidic bonds.…”
Section: Introductionmentioning
confidence: 99%
“…[9][10][11] In recent years, several pretreatment methods have been developed for the fractionation of lignocellulosic biomass. Pretreatment methods include physical pretreatment (mechanical size reduction, pyrolysis), chemical pretreatment (ozonolysis, acid pretreatment, alkali pretreatment, oxidative delignification), physicochemical pretreatment (stream explosion, ammonia fiber explosion, microwave pretreatment, carbon dioxide explosion, wet oxidation) and biological pretreatment (fungus and bacteria) [4], [12], [13][14][15][16][17]. Pretreatment is an essential step prior to saccharification process.…”
Section: Introductionmentioning
confidence: 99%
“…After pretreatment, cellulose is converted to glucose by the cellulase enzymes due the ease in accessibility. Though pretreatment methods have advantages in the fractionation of biomass, each pretreatment method has its own disadvantage, for instance, some pretreatment methods require high energy or use toxic substances or have high operational cost [4].…”
Commonly, the agricultural waste, i.e. lignocellulosic biomass is disposed through combustion causing air pollution with production of PM2.5 and PM10 particles. However, it has been found that these biomasses can be used as source for the production of biofuels and other valuable biochemicals. Though deconstruction of lignocellulosic biomass is challenging due to its complex structure. In this study, rice straw (RS) was pretreated using potassium permanganate (KMnO4) to enhance the enzymatic saccharification efficiency. The study was carried out by varying the operational factors in pretreatment, including temperature (30-90°C), time (30-360 min) and concentration of KMnO4 (0.5-3.0, % w/v), respectively, based on Box-Behnken design (BBD). Through multi-regression analysis of the experimental data obtained after pretreatment, the optimum conditions were determined. The optimum conditions for temperature, time and potassium permanganate concentration were 48.09°C, 360 min, and 1.36% w/v, respectively. The saccharifications of pretreatment and untreated rice straw were carried out using Cellic Ctec2. The reducing sugar was determined by using DNS method and the yields of the untreated and pretreated RS were 32.38 and 49.011 mg/mL, respectively. The results showed that the sugar for pretreated RS were 1.51 fold times higher compared to untreated RS. Therefore, this work illustrates the pretreatment efficiency for KMnO4 to enhance the reducing sugar yield during saccharification, which can be used for biofuel and value-added product productions.
“…The advantages of using biomass are noted for its ability to be stored and used on demand, clean energy, renewable, and no carbon dioxide side effect. In addition, biomass also has the potential to reduce the dependency on fossil fuels, which are the main source of carbon dioxide release in the atmosphere [4][5][6][7].…”
Renewable energy has recently been a promising interest as a substitute for fossil fuels due to an increasing energy demand as well as a rising concern over the environmental impact of fossil fuel consumption around the globe. Biofuel, in particular, is a type of renewable energy, which can be derived from various biomass types. In this research, we analyze relative efficiencies using Data Envelopment Analysis (DEA) technique from three types of energy-related plants in the Northeastern region of Thailand, which are cassava, sugarcane, and palm. The relative efficiency of each province is further analyzed during 2017 to 2019 for a comparative study. Next, the input criteria are collected including allowable planting area, labor cost, and rainfall amount; whereas the included output criterion is the quantity of harvested product. Our initial analysis using CCR, BBC, and Scale Efficiency (SE) models of DEA provides the baseline of efficient provinces to be benchmarked and directions for improving inefficient provinces, given desired input and output criteria in this study.
“…Rice is one of the most economically and nutritionally important cereals, with about 60% of the world's population consuming rice as a basic diet. In addition, rice is the most cultivated crop in the Asia-Pacific region and is the staple food of some developing countries [2] and its by-products could be converted to various valueadded products [3]. Some rice cultivars were characterized to contain beneficial nutrition to improve human health [4].…”
Understanding in population structure of a plant’s root-associated microbiome is applied in good practices in agricultural activities to improve production yields and enhance plant immune responses. The molecular analysis of bacterial populations inhabited in soil faces difficulties in obtaining high yield and high purity of DNA, and different commercial DNA extraction kits have been developed for this purpose. This study focuses on the comparison of DNA extraction of six different rice root-associated bacterial consortium using three commercial kits with two key technologies, spin-column adsorption and magnetic bead adsorption. The quality and quantity of genomic DNA obtained from these extraction methods were analyzed and compared based on DNA concentration, DNA purity and efficiency to be used as a template for 16sRNA amplification. The results showed that the extraction kit with magnetic bead adsorption technology showed the highest concentration (101.32 ng/μl) compared to other DNA extraction kits (32.67 and 1.89 ng/μl). The purity values (A260/A280) were assessed by using Nano-drop spectrophotometer and resulted in purities of nucleic acids in the range of 1.4-1.7. Thus, it was concluded that the extracted DNA obtained from the extraction kit with magnetic bead adsorption technology can be valuable for molecular analysis of microbial communities present in the soil.
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