Recent Research Advances in Biology Vol. 4

Ofosu, Mina; Emmanuel, Boampong Gyekye; Khalilia, Walid; Yahaya, Muhammad Sanusi; Salisi, Mohd Shahrom; Isa, Nur Mahiza Md; Haron, Abd Wahid; Peter, Innocent Damudu; Yetti, Rina Desni; Safni, Safni; Rivai, Harrizul; Wahab, Razak; Edin, Taharah; Mokhtar, Nasihah; Sulaiman, Mohamad Saiful; Ghani, Ros Syazmini Mohd; Razak, Mohammad Haziq; Zrig, Ahlem; Vellani, Vittorio; Giacomoni, Chiara; Chuku, Aleruchi; Nwankiti, Obinna O.; Herrera, Arturo Solís; Singh, Jagdish P.; Goyal, Surbhi; Kumar, Ranjan; Ranieri, Enzo; Fletcher, Janice M.; Ajayi, Clement Olusoji; Elujoba, AA; Bejide, Ronald; Akinloye, Johnson A.; Omonisi, Abidemi

doi:10.9734/bpi/rrab/v4

Cited by 2 publications

(2 citation statements)

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“…The proliferation of HeLa cells was measured by MTT assay as described previously [7]. AI and apoptotic morphological features were assessed by fluorescent microscopy using DAPI staining as described previously [8]. To evaluate mRNA expression in irradiated cells total RNA was extracted using High Pure RNA Isolation kit (Roche) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Gamma Radiation Induces Apoptosis by Over Expression of PERP Effector of p53-dependent Cell Death in HeLa Cells.

Khalilia¹,

Özcan

2021

Proceedings of the 1st International Electronic Conference on Cancers: Exploiting Cancer Vulnerability by Targeting the DNA Dam

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The objectives of this study were to determine the configuration of several genes in response to Gamma Radiation (GR) treatment. Novel treatment strategy to increase the cancer cell sensitivity to radiotherapy by modulation of the PERP expression could be developed for cervical cancers. Cervical cancer cells were incubated for several periods after were exposed to various doses of GR, MTT assay were used to explore propagation of HeLa cells, apoptotic Index (AI) were measured using fluorescent microscopy by estimating apoptotic morphological features. While, signalling pathway analyses were performed on up-regulated genes which evaluated using microarray technology. From the results of this study, the proliferation of HeLa cervical cancer cells exposed to GR was inhibited proportionally with dose and time after exposure. Also, apoptotic morphological features, such as shrinkage of the cell and formation of apoptotic bodies, was clearly visible under the microscope for irradiated HeLa cells. after 48 h. exposure to different doses of GR, the dose of 32 Gy was specified as an AI dose. The mRNA levels of pro-apoptotic genes such as, PERP; BAX; CASP9; TRAF3 and other factors detected by microarray after treatment with GR were up-regulated. Whereas, many anti-apoptosis factors were down-regulated. P53 pathway were significantly reinforced after pathway analysis for up-and dawn-regulated genes. For conclusion, GR induces apoptosis by over expression of PERP factor and p53-dependent cell death in cervical carcinoma HeLa cells.

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Section: Methodsmentioning

confidence: 99%

Gamma Radiation Induces Apoptosis by Over Expression of PERP Effector of p53-dependent Cell Death in HeLa Cells.

Khalilia¹,

Özcan

2021

Proceedings of the 1st International Electronic Conference on Cancers: Exploiting Cancer Vulnerability by Targeting the DNA Dam

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“…The scion assimilates the carbohydrates and translocates them, along with the hormones, to the root system. While the root system can also provide hormones for the scion, as well as water and nutrients absorbed from the soil [97]. Numerous research studies have been done to better understand how rootstock and scion interact to produce traits such as modest tree stature and precocity.…”

Section: Impact Of Scion/rootstock Reciprocal Effectsmentioning

confidence: 99%

Perspective Chapter: Rootstock-Scion Interaction Effect on Improving Salt Tolerance in Fruit Trees

Zrig,

Belhadj,

Tounekti

et al. 2023

Plant Abiotic Stress Responses and Tolerance Mechanisms

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Salt stress is a sever threat to global agriculture. Improving salt tolerance is a problematic task due to the large number of characteristics involved. Graft technique is a potential substitute to breeding and interesting practices to salt tolerance since it unites a scion and rootstock of two genotypes. Increased salinity tolerance in fruit trees will alter water relations, disrupt ionic balance, which can harm plant tissues and thus limit plant productivity. Therefore, the ability of fruit trees to resist salinity varies by species, although it is mostly determined by the type of their root systems. In this regard, the selection of salt-tolerant rootstocks can help maintain productivity under salinity. Several physiological and biochemical changes are attributed to the favorable response of grafting exerted by tolerant rootstocks or scion-rootstock interactions on yield and fruit attributes of plants in saline environments. Rootstocks provide grafted plants different salt tolerance mechanisms including the accumulation of compatible solutes and enhancing the antioxidant mechanisms in scion. The importance of grafting, strategies for selecting appropriate rootstocks, scion-rootstock interaction for growth and the tolerance mechanisms used by plants to avoid the effects of salt stress, are all discussed in this review. Grafting’s potential challenges are also discussed.

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Recent Research Advances in Biology Vol. 4

Cited by 2 publications

References 0 publications

Gamma Radiation Induces Apoptosis by Over Expression of PERP Effector of p53-dependent Cell Death in HeLa Cells.

Gamma Radiation Induces Apoptosis by Over Expression of PERP Effector of p53-dependent Cell Death in HeLa Cells.

Perspective Chapter: Rootstock-Scion Interaction Effect on Improving Salt Tolerance in Fruit Trees

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