Recent Progress of the Golgi Technique and Electron Microscopy To Examine Dendritic Pathology In Alzheimer‘s Disease

Baloyannis, Stavros J.

doi:10.2217/fnl.13.10

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…Samples from the hypothalamus were excised and processed for electron microscopy and silver impregnation techniques including rapid Golgi's method, Rio Hortega's and Bodian's techniques [57,58].…”

Section: Methodsmentioning

confidence: 99%

“…Following dehydration in graded alcohol solutions, the specimens were embedded in paraffin and cut, some of them at 100 μ and some at 25 μ, alternatively [54]. Sections of 25 μ were stained also with methylene blue, according to Golgi-Nissl method [57][58][59][60]. All the sections were mounted in Entellan (Merck-Millipore, Darmstadt, Germany), between two cover slips and studied in a Zeiss Axiolab Photomicroscope, equipped with digital camera and computer.…”

Section: Electron Microscopymentioning

confidence: 99%

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The Hypothalamus in Alzheimer’s Disease: A Golgi and Electron and Microscope Study

Baloyannis¹,

Mavroudis²,

Mitilineos³

et al. 2019

Cognitive Disorders

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Alzheimer's disease is a progressive irreversible neurodegenerative disorder, characterized by gradual decline of mental faculties including learning capacity, emotional and behavioral alterations, serious decline of motor skills, and dysfunction of the autonomic nervous system with disruption of circadian rhythms. Among the potential modifiable risk factors diabetes and obesity may play a considerable role in the pathogenetic background of the disease. We describe some of the morphological alterations of the hypothalamic nuclei in early cases of Alzheimer's disease, using silver impregnation techniques and electron microscopy. The morphological and morphometric study revealed substantial decrease of the neuronal population, which was particularly marked in the suprachiasmatic, the supraoptic and the paraventricular nuclei of the hypothalamus. The silver staining demonstrated an obvious shortage of the dendritic arborization of neurons, associated with marked spinal pathology and axonal dystrophy. It must be underlined that Alzheimer's pathology, such as neuritic plaques and neurofibrillary degeneration was minimal in hypothalamus in comparison with other areas of the brain. Mitochondrial alterations and fragmentation of Golgi complex were observed by electron microscopy in a substantial number of neurons and astrocytes in the hypothalamic nuclei. The hypothalamic pathology may be related to instability of autonomic regulation which occurs gradually in Alzheimer's disease.

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Section: Methodsmentioning

confidence: 99%

Section: Electron Microscopymentioning

confidence: 99%

The Hypothalamus in Alzheimer’s Disease: A Golgi and Electron and Microscope Study

Baloyannis¹,

Mavroudis²,

Mitilineos³

et al. 2019

Cognitive Disorders

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show abstract

“…Golgi techniques contributed to broadening our views on neuronal development, maturation, dendritic and synaptic plasticity, senescence, and degenerating processes. 77…”

Section: Discussionmentioning

confidence: 99%

Spinal Alterations of Reil Insula in Alzheimer’s Disease

Petrides

Mavroudis

Spilioti

et al. 2017

Am J Alzheimers Dis Other Demen

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Alzheimer's disease (AD) is a progressive neurodegenerative disease that involves numerous cellular and biochemical mechanisms resulting in synaptic alterations and extensive neuronal loss. It is primarily characterized by impairment of memory, associated frequently with mood disorders. Continuous studies have shown that insula may be an important target of AD, but neuropathological alterations have not been described extensively. In the present study, we attempted to describe the morphometric and morphological changes of the spines of Reil insula in AD in comparison with normal aging using a silver impregnation technique. We classified spines into 3 types: (1) long neck, (2) short stubby, and (3) other types; and we measured and correlated the length of them in normal controls and in individuals with AD using ImageJ application. Statistical analysis was based on the Student t test on the basis of 360 cells in SPSS v.17.0, and significance was taken as P < .05.

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“…Samples from the hypothalamus were excised and processed for electron microscopy and silver impregnation techniques, including rapid Golgi's method, Golgi-Nissl method, and Rio Hortega and Bodian techniques [55,56].…”

Section: Methodsmentioning

confidence: 99%

The Hypothalamus in Alzheimer’s Disease

Baloyannis¹,

Mavroudis²,

Mitilineos³

et al. 2018

Hypothalamus in Health and Diseases

View full text Add to dashboard Cite

Alzheimer's disease is a progressive, irreversible neurodegenerative disorder, characterized by gradual decline of mental faculties, including learning capacity, emotional and behavioral alterations, serious decline of motor skills, and dysfunction of the autonomic nervous system with disruption of circadian rhythms. Among the potential modifiable risk factors, diabetes and obesity may play a considerable role in the pathogenetic background of the disease. We describe some of the morphological alterations of the hypothalamic nuclei in early cases of Alzheimer's disease, using silver impregnation techniques and electron microscopy. The morphological and morphometric study revealed substantial decrease of the neuronal population, which was particularly marked in the suprachiasmatic, the supraoptic, and the paraventricular nuclei of the hypothalamus. The silver staining demonstrated an obvious shortage of the dendritic arborization of neurons, associated with marked spinal pathology and axonal dystrophy. It must be underlined that Alzheimer's pathology, such as neuritic plaques and neurofibrillary degeneration, was minimal in the hypothalamus in comparison with other cortical and subcortical areas of the brain. Mitochondrial alterations and fragmentation of Golgi complex were observed by electron microscopy in a substantial number of neurons and astrocytes in the hypothalamic nuclei. The hypothalamic pathology may be related to instability of autonomic regulation which occurs gradually in Alzheimer's disease.

show abstract

Recent Progress of the Golgi Technique and Electron Microscopy To Examine Dendritic Pathology In Alzheimer‘s Disease

Cited by 11 publications

References 27 publications

The Hypothalamus in Alzheimer’s Disease: A Golgi and Electron and Microscope Study

The Hypothalamus in Alzheimer’s Disease: A Golgi and Electron and Microscope Study

Spinal Alterations of Reil Insula in Alzheimer’s Disease

The Hypothalamus in Alzheimer’s Disease

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