Recent progress of functionalized nanomaterials-based biosensing for monitoring of food- and water-borne pathogens

Dey, Piyush; Raza, Mohd Jamshaiya; Khera, Alisha; Sharma, Akanksha; Khajuria, Akhil; Pandey, Ankur; Pandey, Chandra Mouli; Sharma, Rakesh Kumar; Singh, Gurpal; Barnwal, Ravi Pratap

doi:10.1016/j.enmm.2024.100914

Cited by 2 publications

(1 citation statement)

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“…Plastic pollution in aquatic ecosystems poses a severe threat Antibiotics 2024, 13, 339 2 of 12 to planetary health as commercial plastic production has increased drastically over the last 60 years, reaching levels of up to 300 million tons per year, and it may soon surpass the total fish biomass in aquatic ecosystems [8]. The transmission of plastic-associated antibiotic-resistant bacteria (ARB) in aquatic ecosystems is an emerging issue, and the link between plastic pollution, the spread of pathogenic bacteria, and human health problems is widely reported in the literature [9,10].…”

Section: Introductionmentioning

confidence: 99%

Emerging Issues on Antibiotic-Resistant Bacteria Colonizing Plastic Waste in Aquatic Ecosystems

Ferheen,

Spurio,

Marcheggiani

2024

Antibiotics

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Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant human pathogens, Staphylococcus spp. and Klebsiella spp., and their sophisticated equipment of antibiotic-resistant genes (ARGs), retrieved from plastic substrates submerged into an inland water body. The results of microbiological analysis on selective and chromogenic media revealed the presence of colonies with distinctive phenotypes, which were identified using biochemical and molecular methods. 16S rDNA sequencing and BLAST analysis confirmed the presence of Klebsiella spp., while in the case of Staphylococcus spp., 63.6% of strains were found to be members of Lysinibacillus spp., and the remaining 36.3% were identified as Exiguobacterium acetylicum. The Kirby–Bauer disc diffusion assay was performed to test the susceptibility of the isolates to nine commercially available antibiotics, while the genotypic resistant profile was determined for two genes of class 1 integrons and eighteen ARGs belonging to different classes of antibiotics. All isolated bacteria displayed a high prevalence of resistance against all tested antibiotics. These findings provide insights into the emerging risks linked to colonization by potential human opportunistic pathogens on plastic waste commonly found in aquatic ecosystems.

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