Recent progress in the synthesis of all-aqueous two-phase droplets using microfluidic approaches

Daradmare, Sneha; Lee, Chang‐Soo

doi:10.1016/j.colsurfb.2022.112795

Cited by 16 publications

(9 citation statements)

References 228 publications

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“…In this present work, SA/DEX, PEG, and chitosan/PEG solutions were employed as core, middle, and shell phase solutions. In the past, because of the ultralow interfacial tension between water-water phases, researchers have tried active-microfluidic methods that involve the use of external perturbation to generate the droplets (Chao et al, 2020;Daradmare et al, 2022). A pneumatic valve that converts mechanical energy into surface energy partially addressed the limitation of ultralow interfacial tension between water-water phases (Wang et al, 2020).…”

Section: Design and Manipulation Of Atps Droplet Microfluidic Platformmentioning

confidence: 99%

“…However, the major challenge of using ATPS in microfluidic systems is the ultralow interfacial surface tension between two aqueous phases resulting in unstable droplets formation (Sauret et al, 2012;Azizian et al, 2019;Beldengrün et al, 2020). But, recent investigations have shown numerous ways for example, passive and active methods to stabilize the droplets within the microfluidic devices (Chao et al, 2020;Daradmare et al, 2022). The successful generation of w/w droplets within microfluidic devices provides a great opportunity to utilize these uniformsized droplets in various applications, such as biological hosts, reactors, and templates in the synthesis of biomaterials (Chao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, researchers have attempted several strategies for the stabilization of w/w emulsion droplets that prevent droplet coalescence (Chao et al, 2020;Daradmare et al, 2022). Most of the strategies such as the use of synthesizing customized macromolecular polymers and different kinds of colloidal particles have been employed in bulk ATPS emulsification to stabilize w/w emulsion droplets (Buzza et al, 2013;Binks et al, 2017;Dumas et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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One-step on-chip microfluidic synthesis of the hybrid capsules using aqueous two-phase system

Daradmare¹,

Kim

Ganguly³

et al. 2022

Front. Sens.

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Hydrogel capsules synthesized by conventional water-in-oil emulsion systems are the less preferred choice for biomedical applications due to the use of oils and surfactants. An aqueous two-phase system (ATPS), which allows the formation of water-in-water emulsion, is considered a green alternative and therefore has been explored a lot for its application in the biomedical field. Herein, we present the synthesis of hydrogel capsules using a set-up consisting of a pneumatic valve integrated with the ATPS microfluidic system. In this arrangement, at first, a pneumatic valve facilitates the generation of the droplets of one aqueous system i.e. sodium alginate (SA) containing dextran solution into another aqueous phase comprising polyethylene glycol solution. The present approach allows good control over droplet generation by tuning the pressure of the pneumatic valve and the flow rates of the core and middle phases. The synthesis of hybrid capsules within the microfluidic device is carried out mainly by using the interfacial complexation of oppositely charged polyelectrolytes, chitosan with SA via electrostatic interactions. The interfacial complexed SA and chitosan hydrogel capsules were collected via the settling collection method, which ensures the retaining of the shape of the hybrid capsules. The morphological properties of as-synthesized droplets and hybrid capsules were examined via optical microscopy. The hydrogel capsules show good encapsulation capability for the magnetic particles. Even though this study mainly focuses on the synthesis part, we anticipate that the proposed approach will enable the encapsulation of cells within the hybrid capsules as well as enhance the cell adhesion on the surface of the hydrogel capsules hence, these hydrogel capsules can find the potent application in the biomedical engineering.

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Section: Design and Manipulation Of Atps Droplet Microfluidic Platformmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

One-step on-chip microfluidic synthesis of the hybrid capsules using aqueous two-phase system

Daradmare¹,

Kim

Ganguly³

et al. 2022

Front. Sens.

Self Cite

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show abstract

“…Bioactive molecules and cells largely preserve their native structures and biological functions in the all-aqueous environment. 4,5 Consequently, W/W emulsions are more advantageous over traditional water/oil emulsions for processing of biomolecules, 1,4,6,7 and they are increasingly exploited as liquid scaffolding for patterning of cells, culture of organoids and printing of tissues. [8][9][10][11][12][13] W/W interface are highly permeable to oxygen, nutrients, metabolic waste, and even living cells.…”

Section: Introductionmentioning

confidence: 99%

All-aqueous Synthesis of Fibrillated Protein Microcapsules for Membrane-bounded Culture of Tumor Spheroids

Wei

Cai

Yang

et al. 2023

Preprint

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Water-in-water (W/W) emulsion provides cytocompatible compartments for cell encapsulation and 3D printing of tissues. Formation of semi-permeable membrane at the W/W interface is critical to entrapment of cells in the droplet phase and supply of nutrients from the continuous phase. However, the adsorption of colloidal particles at the W/W interface is dynamic and reversible, which represents an inherent limitation for fabrication of mechanically robust cell-laden microcapsules. Here we demonstrate the preparation of thin, inflatable and semi-permeable microcapsules by using clusters of protein fibrils as building blocks and control their assembly at W/W interface. These fibril clusters are prepared by cross-linking lysozyme fibrils with multi-arm polyethylene glycol (PEG) through click chemistry. Compared to linear-structured fibrils, fibril clusters can strongly adsorb at W/W interface, and they packed into an interconnected meshwork to stabilize W/W emulsion. Moreover, when fibril clusters are complexed with calcium alginate, the hybrid microcapsules can bear a high osmotic gradient of 60 mOsm/Kg induced by dextran (Mw=500,000), and their surface area expand by over 100% without rupture. This all-aqueous biomaterial synthesis approach allows fabrication of mechanically robust capsules for long-time culture of SGC-996 tumor spheroids, with great potentials to be used in anti-tumor drug-screening and tissue transplantation.

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“…The phase interface is a promising tool to confine chemical species. Among all the interfaces, the interface of aqueous two-phase systems (ATPS) has a unique superiority: phases on both sides of the interface show good biocompatibility, – enabling various cellular applications. – Biomolecules usually have preference over one phase of ATPS, ,, and the partition properties can be extensively adjusted, – bringing wide applications in extraction, separation, and microreactors. ,, Low interfacial tensions is a disadvantage for conventional ATPS droplet applications, ,, but it makes the two phases favor stable laminar flow, facilitating the manipulation by a microfluidic probe. However, even though the chemical species has a preference for one phase, it will still diffuse to the other phase until the partition ratio is reached .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

Zhang,

Xie,

et al. 2023

ACS Appl. Mater. Interfaces

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Confinement of chemical species in a controllable micrometer-level (several to a dozen micrometers) space in an aqueous environment is essential for precisely manipulating chemical events in subcellular regions. However, rapid diffusion and hard-to-control micrometer-level fluids make it a tough challenge. Here, a versatile open microfluidic method based on an aqueous two-phase system (ATPS) is developed to restrict species inside an open space with micron-level width. Unequal standard chemical potentials of the chemical species in two phases and space-time correspondence in the microfluidic system prevent outward diffusion across the phase interface, retaining the target species inside its preferred phase flow and creating a sharp boundary with a dramatic concentration change. Then, the chemical flow (the preferred phase with target chemical species) is precisely manipulated by a microfluidic probe, which can be compressed to a micron-level width and aimed at an arbitrary position of the sample. As a demonstration of the feasibility and versatility of the strategy, chemical flow is successfully applied to subcellular regions of various kinds of living single cells. Subcellular regions are successfully labeled (cytomembrane and mitochondria) and damaged. Healing−regeneration behaviors of living single cells are triggered by subcellular damage and analyzed. The method is relatively general regarding the species of chemicals and biosamples, which could promote deeper cell research.

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Recent progress in the synthesis of all-aqueous two-phase droplets using microfluidic approaches

Cited by 16 publications

References 228 publications

One-step on-chip microfluidic synthesis of the hybrid capsules using aqueous two-phase system

One-step on-chip microfluidic synthesis of the hybrid capsules using aqueous two-phase system

All-aqueous Synthesis of Fibrillated Protein Microcapsules for Membrane-bounded Culture of Tumor Spheroids

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

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