Recent progress in the fundamental understanding of hydrophilic interaction chromatography (HILIC)

Guo, Yong

doi:10.1039/c5an00670h

Cited by 116 publications

(59 citation statements)

References 65 publications

(224 reference statements)

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“…In HILIC, an aprotic solvent such as acetonitrile embracing small amounts of water is used as the mobile phase and compounds are eluted based on increasing hydrophilicity 45–47 . Although the retention mechanism of HILIC is still not fully understood, electrostatic interactions and adsorption, as well as liquid partitioning, are likely involved 45–47 . Clearly, retention of an analyte is related to the specific mobile phase, the functional groups present on the analyte, and the stationary phase column chemistry 45–47 .…”

Section: Analytical Toolbox Of Chromatographic Modesmentioning

confidence: 99%

“…Although the retention mechanism of HILIC is still not fully understood, electrostatic interactions and adsorption, as well as liquid partitioning, are likely involved 45–47 . Clearly, retention of an analyte is related to the specific mobile phase, the functional groups present on the analyte, and the stationary phase column chemistry 45–47 . Moreover, HILIC-based stationary phases offer high selectivity and increased compatibility with mass spectrometry (MS) as compared to other normal-phase supports 46 .…”

Section: Analytical Toolbox Of Chromatographic Modesmentioning

confidence: 99%

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Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates

2017

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Section: Analytical Toolbox Of Chromatographic Modesmentioning

confidence: 99%

Section: Analytical Toolbox Of Chromatographic Modesmentioning

confidence: 99%

Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates

2017

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“…The retention mechanism is mainly based on hydrophilic partitioning of polar compounds between a water-enriched layer formed at the surface of the stationary phase and the highly organic mobile phase. Depending on the nature of both stationary and mobile phases, as well as the physico-chemical properties of the analytes, additional interaction mechanisms can occur besides hydrophilic partitioning, including hydrogen bonding, dipole-dipole interactions, and ionic interactions [15][16][17][18]. In the context of bioanalysis, HILIC-MS/MS presents two main advantages.…”

Section: Introductionmentioning

confidence: 99%

Systematic evaluation of matrix effects in hydrophilic interaction chromatography versus reversed phase liquid chromatography coupled to mass spectrometry

Periat

Köhler

Thomas

et al. 2016

Journal of Chromatography A

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“…The basic types of HILIC columns include plain silica, neutral, polar chemically bonded, ion-exchange and zwitterionic stationary phases [19,20]. The retention mechanism in HILIC chromatography is complex involving hydrophilic partition, absorption and electrostatic interactions and further studies are needed to elucidate this [21][22][23].…”

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confidence: 99%

Investigation of the Retention Mechanism of Cephalosporins by Zwitterionic Hydrophilic Interaction Liquid Chromatography

Panderi¹,

Malamos²,

Machairas³

et al. 2016

Chromatographia

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adverse effects on human health, such as resistance development and allergic reactions [1][2][3][4]. There is no doubt that the importance of cephalosporin antibiotics in medicine requires the development of high performance separation methods for their analysis [5,6]. Literature survey revealed that numerous chromatographic methods have been developed for the analysis of cephalosporins including isocratic or gradient techniques coupled with various methods of detection [7][8][9][10][11][12][13][14]. These compounds are highly polar molecules and represent perfect candidates to study the retention mechanism in hydrophilic interaction liquid chromatography (HILIC). Up to now research on the retention of cephalosporins in HILIC has been focused only on the use of plain silica columns [15,16]. The zwitterionic hydrophilic interaction liquid chromatography used in this study is a unique form of HILIC which involves the use of stationary phases with zwitterionic functional groups covalently bonded at a silica or polymeric substrate.HILIC chromatography is an alternative chromatographic technique using polar stationary phases and low aqueous/high organic mobile phases in order to achieve effective retention for polar analytes. It appears to be complementary to reversed-phase LC as the order of elution is reversed to reversed-phase LC and thus it provides reasonable retention for polar compounds [17,18]. When developing robust methods in HILIC, it is important to understand the dominant mechanism that governs retention which is related to the physicochemical characteristics of the analytes and to the analytical column substrate. The basic types of HILIC columns include plain silica, neutral, polar chemically bonded, ion-exchange and zwitterionic stationary phases [19,20]. The retention mechanism in HILIC chromatography is complex involving hydrophilic partition, absorption and electrostatic interactions and further studies are needed to elucidate this [21][22][23]. AbstractThe importance of cephalosporin antibiotics in medicine requires the development of high performance separation methods for their analysis. In this paper cefazolin, cefuroxime, cefoxitin, cefaclor and ceftriaxone were used as model compounds to investigate the retention mechanism under zwitterionic HILIC conditions. The analysis was performed using a ZIC ® -HILIC analytical column (150.0 × 2.1 mm i.d., particle size 3.5 μm, 200 Å) with isocratic elution. The mobile phase was a mixture of acetonitrile and ammonium acetate or formate buffer. To get a better insight into the retention mechanism on ZIC ® -HILIC columns the main factors influencing the retention and selectivity of the analytes have been thoroughly investigated. It is shown that the type of the salt and its concentration along with the volume fraction of water in the mobile phase and pH of the aqueous buffer greatly affect the retention of the tested cephalosporins due to their structures and physicochemical properties. The method was applied successfully to the analysis of injection dosage...

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Recent progress in the fundamental understanding of hydrophilic interaction chromatography (HILIC)

Cited by 116 publications

References 65 publications

Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates

Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates

Systematic evaluation of matrix effects in hydrophilic interaction chromatography versus reversed phase liquid chromatography coupled to mass spectrometry

Investigation of the Retention Mechanism of Cephalosporins by Zwitterionic Hydrophilic Interaction Liquid Chromatography

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