Recent Progress in the Detection of Bacteria Using Bacteriophages: A Review

Paczesny, Jan; Richter, Łukasz; Hołyst, Robert

doi:10.3390/v12080845

Cited by 45 publications

(34 citation statements)

References 108 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also essential to explore the rate at which bacteria develop or lose “immunity” towards phages. Another concern is the accurate identification of the pathogen causing the infection, as phages are extremely specific in their action [ 187 ]. Further complications in the application of this therapy, e.g., combination with antibiotics, and in-patient care, also require consideration [ 188 ].…”

Section: Bacteriophagesmentioning

confidence: 99%

Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages

et al. 2021

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance is a significant threat to human health worldwide, forcing scientists to explore non-traditional antibacterial agents to support rapid interventions and combat the emergence and spread of drug resistant bacteria. Many new antibiotic-free approaches are being developed while the old ones are being revised, resulting in creating unique solutions that arise at the interface of physics, nanotechnology, and microbiology. Specifically, physical factors (e.g., pressure, temperature, UV light) are increasingly used for industrial sterilization. Nanoparticles (unmodified or in combination with toxic compounds) are also applied to circumvent in vivo drug resistance mechanisms in bacteria. Recently, bacteriophage-based treatments are also gaining momentum due to their high bactericidal activity and specificity. Although the number of novel approaches for tackling the antimicrobial resistance crisis is snowballing, it is still unclear if any proposed solutions would provide a long-term remedy. This review aims to provide a detailed overview of how bacteria acquire resistance against these non-antibiotic factors. We also discuss innate bacterial defense systems and how bacteriophages have evolved to tackle them.

show abstract

Section: Bacteriophagesmentioning

confidence: 99%

Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Phage-based biosensors employ bacteriophage as the recognition element (5,34,66,88). The advantage of this approach owes to the unique characteristics of phages.…”

Section: Phage-based Biosensorsmentioning

confidence: 99%

Current State of Development of Biosensors and Their Application in Foodborne Pathogen Detection

Bai

Bhunia

2021

Journal of Food Protection

View full text Add to dashboard Cite

Foodborne disease outbreaks continue to be a major public health and food safety concern. Ensuring the safety of food prior to retail distribution by testing products promptly can protect consumers from foodborne diseases. F ast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored in the past few years to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1-100 bacterial cells or pico-nanogram levels of toxins). Besides, advancement in mammalian cells and bacteriophage-based sensors led to their ability to detect not only low levels of pathogens but also to differentiate live from dead ones. Combining different biotechnology platforms enabled practical utility and application of biosensors in foodborne pathogen detection. However, further rigorous testing of biosensors from complex food matrices is needed to ensure their utility in point-of-care need and for outbreak investigations.

show abstract

“…There are many more examples of how phages are used for pathogen detection for non-biothreat organisms that are well reviewed [2][3][4][6][7][8]. This is in part due to the greater interest in bacteria that cause food borne illness and other common bacterial pathogens that are easier to work with in the laboratory and do not have biosecurity concerns.…”

Section: Future Directionsmentioning

confidence: 99%

“…From the Center for Disease Control and Prevention's (CDC) gold standard for "phage typing" bacterial isolates to the development of the phage display system for discovery of binding peptides and antibodies, researchers have found creative ways to exploit phages for novel pathogen detection platforms. There are many reviews on the use of phages for pathogen detection using different assays, platforms and biosensors [1][2][3][4][5][6][7][8][9]. This review will focus on how phages have been exploited for detection of those pathogens that are of most concern due to their history and potential for use as biowarfare and bioterrorism agents.…”

Section: Introductionmentioning

confidence: 99%

The State of the Art in Biodefense Related Bacterial Pathogen Detection Using Bacteriophages: How It Started and How It’s Going

Sozhamannan

Hofmann

2020

Viruses

View full text Add to dashboard Cite

Accurate pathogen detection and diagnosis is paramount in clinical success of treating patients. There are two general paradigms in pathogen detection: molecular and immuno-based, and phage-based detection is a third emerging paradigm due to its sensitivity and selectivity. Molecular detection methods look for genetic material specific for a given pathogen in a sample usually by polymerase chain reaction (PCR). Immuno-methods look at the pathogen components (antigens) by antibodies raised against that pathogen specific antigens. There are different variations and products based on these two paradigms with advantages and disadvantages. The third paradigm at least for bacterial pathogen detection entails bacteriophages specific for a given bacterium. Sensitivity and specificity are the two key parameters in any pathogen detection system. By their very nature, bacteriophages afford the best sensitivity for bacterial detection. Bacteria and bacteriophages form the predator-prey pair in the evolutionary arms race and has coevolved over time to acquire the exquisite specificity of the pair, in some instances at the strain level. This specificity has been exploited for diagnostic purposes of various pathogens of concern in clinical and other settings. Many recent reviews focus on phage-based detection and sensor technologies. In this review, we focus on a very special group of pathogens that are of concern in biodefense because of their potential misuse in bioterrorism and their extremely virulent nature and as such fall under the Centers for Disease and Prevention (CDC) Category A pathogen list. We describe the currently available phage methods that are based on the usual modalities of detection from culture, to molecular and immuno- and fluorescent methods. We further highlight the gaps and the needs for more modern technologies and sensors drawing from technologies existing for detection and surveillance of other pathogens of clinical relevance.

show abstract

Recent Progress in the Detection of Bacteria Using Bacteriophages: A Review

Cited by 45 publications

References 108 publications

Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages

Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages

Current State of Development of Biosensors and Their Application in Foodborne Pathogen Detection

The State of the Art in Biodefense Related Bacterial Pathogen Detection Using Bacteriophages: How It Started and How It’s Going

Contact Info

Product

Resources

About