Recent progress in the analysis of sugar monomers from complex matrices using chromatography in conjunction with mass spectrometry or stand-alone tandem mass spectrometry

Fractionation and characterization of polysaccharides from cyanobacterium Spirulina (Arthrospira) maxima in nitrogen-limited batch culture

“…The actual molar ratios of monosaccharides of the four fractionated polysaccharides are shown in Table 2, suggesting that different potential applications of these fractionated sugars, e. g., PSSG and PSCW may be the better source for production of highadded value glucose (e. g., isotopically labeled glucose), while PSEL and PSCM is suitable for production of the mixture of sugars that may have biopharmaceutical or healthy functions El ' (3,4.8).xylose (5,6). matmoae (11,13), galaettee (12,14,16), glucose (19,20,23), 8alactuz~c acid (10,12,17,18), glucun./c acid (9,21); (b)---mamfitol (4), glucoee (2,3,5), unknown (1); (c)~matmitol (5), gluo0ee (3,4,6), unknown (1,2); (d)---maanitol (21), thanH~ (4,5), futx~ (6,7,10), xyloee (8,9), ~ (12), 8alactase (13,14,16), glucose (18,19,22), 8aIacammie acid (11,17), glucuronic acid (20), unknown (1,2,3); (e)--manmtal (23), duennoae (6,7), fucose (8,9,12), xylose (10,11), ~ (14,15), 8alactoee (16,18), glucoee (20,21,24), ~lu~onic acid (13,19), gluacan~e acid (22) that was harvested at the end of stationary phase from a nutrient nitrogen-limited culture can be quantitatively extracted by the proposed sequentially selective extraction procedure that mairdy consists of water treatments. Ommcterization of the fractionated polysaccharides by GLC, with derivatization by TMS and methanolysis by 1 mol" L-1 methanol HC1, shows that monosaccharides in PSSG and PSCW are almost only gluco...…”

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

“…Although numerous methods are available for monitoring enzyme activities they all have some limitations. Well-established techniques based on chromatography combined with mass spectrometry are available for assessing the oligomeric fragments of glycosyl hydrolase (GH) activities 3,4 . However, these approaches are labor intensive and generally low-throughput.…”

Determination of sugar compounds in atmospheric aerosols by liquid chromatography combined with positive electrospray ionization mass spectrometry

“…The existing analytical methods for sugar compounds fall into four categories, GCbased methods, high performance liquid chromatography (HPLC)-based methods, direct MS methods, and LC-MS methods (15). The GC-based methods require derivatization of the hydroxyl groups.…”