Recent progress in separation prediction of counter‐current chromatography

Guo, Yuru; Tong, Shengqiang; Zhang, Keqing; Yan, Jizhong

doi:10.1002/jssc.202000473

Cited by 10 publications

(6 citation statements)

References 92 publications

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“…The K value is the ratio of the target compound between both solvent phases. The counter-current chromatography "sweet spot" may be described as an area where K values are between 0.5 and 1.6 A compound with a smaller K value elutes closer to the solvent front with lower resolution while compounds with higher K values have higher resolution but longer elution time [135]. Moreover, the separation factor (α) between two compounds, i.e., the ratio of partition coefficients between two solutes, should be greater than 1.5 for successful separation [133].…”

Section: Counter-current Chromatographymentioning

confidence: 99%

Alternative Extraction and Downstream Purification Processes for Anthocyanins

et al. 2022

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Anthocyanins are natural pigments displaying different attractive colors ranging from red, violet, to blue. These pigments present health benefits that increased their use in food, nutraceuticals, and the cosmetic industry. However, anthocyanins are mainly extracted through conventional methods that are time-consuming and involve the use of organic solvents. Moreover, the chemical diversity of the obtained complex extracts make the downstream purification step challenging. Therefore, the growing demand of these high-value pigments has stimulated the interest in designing new, safe, cost-effective, and tunable strategies for their extraction and purification. The current review focuses on the potential application of compressed fluid-based (such as subcritical and supercritical fluid extraction and pressurized liquid extraction) and deep eutectic solvents-based extraction methods for the recovery of anthocyanins. In addition, an updated review of the application of counter-current chromatography for anthocyanins purification is provided as a faster and cost-effective alternative to preparative-scale HPLC.

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Section: Counter-current Chromatographymentioning

confidence: 99%

Alternative Extraction and Downstream Purification Processes for Anthocyanins

et al. 2022

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“…Counter-current chromatography (CCC) is a nonsolid phase support chromatography technology based on the principle of liquid-liquid distribution [1,2]. Hence, CCC can reduce sample molecule interactions with the stationary phase and eliminate side effects such as irreversible adsorption of the stationary phase to the sample [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Q-TOF-MS [M+Na] + 563.1745, calculated for C 25 H 32 O 13 m/z 540.1745, whereas it was identified as the oleuropein by standard substance comparison. It was further identified as oleuropein by1 HNMR and…”

mentioning

confidence: 99%

Comprehensive separation of a wide variety of compounds from olive leaves by counter‐current chromatography with three‐phase solvent system

Jing

Sun

Wang

et al. 2022

J of Separation Science

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The three‐phase solvent system counter‐current chromatography has been of great research interest, because it can separate compounds with a wide range of polarity. The solvent system of n‐hexane/methyl tert‐butyl ether/acetonitrile/water (5:5:7:5, v/v) was used for counter‐current chromatographic comprehensive separation of olive leaves. The study adopted the normal elution mode. The middle phase and the lower phase (at a volume ratio of 7:3) were pumped into the column simultaneously, followed by eluting with the upper, middle, and lower phases in sequence. The retention rate of the stationary phase measured by the experiment was 73.5%. The upper phase was used to elute the nonpolar compounds, then the mobile phase was switched to the middle phase to elute the moderately hydrophobic compounds, finally, the polar compounds were eluted by the lower phase remaining in the chromatographic column. This method successfully separated eight compounds in one step within 270 min and five compounds were identified. The logP values of these five compounds were 7.44, 7.86, 4.16, −0.11, and 0.96, respectively, covering a wide range of polarities. The present study demonstrated that the three‐phase solvent has a strong extraction capacity for ingredients from extremely hydrophilic compounds to extremely hydrophobic compounds.

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“…Countercurrent chromatography (CCC) is a unique liquid-liquid partition chromatography technique that does not utilize a solid support in the system [8]. CCC is now accepted as an efficient preparative technique and is widely implemented for the separation and purification of various natural and synthetic compounds [8,9]. However, using solely CCC will not separate all chemical components.…”

Section: Introductionmentioning

confidence: 99%

Ionic‐liquid‐based ultrasound‐assisted extraction combined with countercurrent chromatography and semipreparative LC for the preparation of monoamine oxidase B inhibitors from Pueraria thomsonii

Wang

et al. 2022

J of Separation Science

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A simple and efficient method was developed for the rapid screening and identification of ligands for monoamine oxidase B. A new ionic‐liquid‐based ultrasound‐assisted extraction method for medicinal herbs was also developed and validated. In addition, the hyphenated technique of countercurrent chromatography and semipreparative‐LC was developed and applied to the isolation of the chemical constituents for Pueraria thomsonii Benth. Three potent monoamine oxidase B inhibitors, namely, daidzein‐4′,7‐diglucoside (42.2 mg), puerarin 6′′‐O‐xyloside (88.3 mg), and 3′‐hydroxypuerarin (48.5 mg) with purities of 98.2, 96.3, and 97.1%, respectively, were obtained from 500 g of P. thomsonii raw material using semi‐preparative high‐performance liquid chromatography, whereas 3′‐methoxypuerarin (76.2 mg), daidzein‐8‐C‐apiosyl (1→6) glucoside (84.2 mg), and tectorigenin (75.1 mg) with purities of 98.5, 96.4, and 96.8%, respectively, were obtained from 500 g raw material via countercurrent chromatography using a two‐phase solvent system comprising n‐hexane–ethyl acetate–methanol–water at a volume ratio of 1.85:1.00:0.86:3.69 (v/v/v/v). Then, the anti‐Alzheimer activity of the phytochemicals was assessed using a PC12 cell model. Treatment with tectorigenin, daidzein‐4′,7‐diglucoside, puerarin 6′′‐O‐xyloside, 3′‐hydroxypuerarin, 3′‐methoxypuerarin, and daidzein‐8‐C‐apiosyl (1→6) glucoside (100 μg/mL), resulted in cell viabilities of 69.00, 65.81, 59.69, 57.90, 55.61, and 54.59%, respectively (p < 0.001). The protocol was proved to be very accurate and efficient.

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Recent progress in separation prediction of counter‐current chromatography

Cited by 10 publications

References 92 publications

Alternative Extraction and Downstream Purification Processes for Anthocyanins

Alternative Extraction and Downstream Purification Processes for Anthocyanins

Comprehensive separation of a wide variety of compounds from olive leaves by counter‐current chromatography with three‐phase solvent system

Ionic‐liquid‐based ultrasound‐assisted extraction combined with countercurrent chromatography and semipreparative LC for the preparation of monoamine oxidase B inhibitors from Pueraria thomsonii

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