Recent progress in research on cell-to-cell movement of rice viruses

Hiraguri, Akihiro; Netsu, Osamu; Sasaki, Nobumitsu; Nyunoya, Hiroshi; Sasaya, Takahide

doi:10.3389/fmicb.2014.00210

Cited by 9 publications

(4 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies, degradation of callose by ß-1,3-glucanase increases the plasmodesmata (Pd) size exclusion limit (SEL) and facilitates cell-to-cell movement of RNA viruses [31, 32]. This indicates that defense responses and Pd gate modification mechanisms are generally conserved plant responses to RNA viruses [33, 34]. …”

Section: Discussionmentioning

confidence: 99%

RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis

Sun

Peng

et al. 2016

Virol J

View full text Add to dashboard Cite

BackgroundVirus infection induces and suppresses host gene expression on a global level. Rice stripe virus (RSV) is the type species of the genus Tenuivirus and infects rice and Arabidopsis plants. Microarray-based and next generation sequencing-based transcriptomic approaches have been used to study rice-RSV interactions. However, our knowledge of the response of Arabidopsis plants to RSV infection is limited, and it requires further investigation to determine the similarities (or differences) in virus-host interactions between monocot and dicot hosts infected with RSV.MethodsWe characterized transcriptome changes in Arabidopsis thaliana infected with rice stripe virus (RSV) with RNA-seq based digital gene expression (DGE) analysis. The transcriptomes of RSV-infected samples were compared to those of mock-treated samples at 14 and 21 days post-infection (dpi) during different stages of symptom development.ResultsWe identified 624 differentially expressed genes (DEGs) in Arabidopsis influenced by RSV at 14 dpi and 21 dpi, among which at 14 dpi, 255 transcripts were induced, and 38 were repressed; at 21 dpi, 146 were induced, and 237 were repressed. Functional annotation indicated that these DEGs were related to multiple biological functions, including defense response, secondary metabolism, protein amino acid phosphorylation and response to abiotic stress.ConclusionsImportantly, the transcription of genes related to host defense systems was activated by RSV infection at an early stage of symptom development (14 dpi), whereas over the infection period (21 dpi), the host defense response systems were suppressed. A total of 52 genes were continuously differentially expressed between the two time points, indicating that the majority of DEGs were transient and unique to a particular time point during symptom development. The DEGs, particularly the defense response genes, identified in this study are candidates suitable for further functional analysis during the RSV-Arabidopsis interaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0663-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis

Sun

Peng

et al. 2016

Virol J

View full text Add to dashboard Cite

show abstract

“…This insect causes a decrease in crop yield, productive tillers and plant stature. GRL not only causes yield reduction in rice, but it also serves as a vector for viruses, such as rice yellow stunt virus (RYSV), southern rice black‐streaked dwarf virus (SRBSDV) and rice dwarf virus (RDV), all transmitted during the feeding process (Hiraguri, Netsu, Sasaki, Nyunoya, & Sasaya, ).…”

Section: Introductionmentioning

confidence: 99%

Repellency of essential oils against Nephotettix cincticeps: Laboratory and glasshouse assays

Long

Liu

Zhao

et al. 2017

J Applied Entomology

View full text Add to dashboard Cite

Four oils from Piper nigrum, Litsea cubeba, Zanthoxylum bungeanum and Curcuma longa were obtained by ethanol extraction. The repellency of these oils and two major compounds (linalool and piperine) was evaluated against female adult and third-instar nymphs of the rice pest, Nephotettix cincticeps, under laboratory and glasshouse conditions. Paired-choice and no-choice assays were used for each treatment, with essential oils evaluated after 24 and 48 hr of exposure and chemical compounds evaluated after 12 and 24 hr of exposure. The potential effects of essential oils on activities of glutathione S tranferase (GST), carboxyl esterase (CarE) and acetyl cholinesterase (AchE) were also evaluated after 48 hr of exposure to leafhoppers. The constituents of the essential oils were determined using GC-MS. The results showed that the major components in the oils were piperine (34.75%) for P. nigrum, 9,12-octadecadienoic acid (Z,Z) (18.74%) for L. cubeba, ethanone, 1-(2-hydroxy-4,6-dimethoxyphenyl) (18.51%) for Z. bungeanum and turmerone (15.89%) for C. longa. In all cases, the essential oils repelled female adults and third-instar nymphs of N. cincticeps. The repellency of the tested oils and chemicals compounds in the paired-choice assay was higher than in the no-choice assay. In all experimental conditions, P. nigrum and C. longa oils were the most and the least potent, respectively. Linalool was the best repellent among the single-tested compounds under laboratory conditions. In the glasshouse study, the highest repellency was observed in the mixture of linalool and piperine. GST and CarE activities of leafhoppers were significantly enhanced by exposure to the four essentials oils; AchE activity increased significantly only in the P. nigrum and L. cubeba assays. Ourresults clearly indicate that the tested oils and chemical compounds are promising agents for developing plant-based pesticides to control N. cincticeps. K E Y W O R D Senzyme activity, essential oils, leafhopper, repellent

show abstract

“…Intercellular bridges can also be hijacked by pathogens to infect new host cells. TNTs can be involved in the spread of HIV and prions, and plasmodesmata are used by several viruses to spread through the host plant 13 – 15 . Prokaryotes are also capable of direct exchange of macromolecules via intercellular bridges.…”

Section: Introductionmentioning

confidence: 99%

Flagellar membrane fusion and protein exchange in trypanosomes; a new form of cell-cell communication?

et al. 2016

View full text Add to dashboard Cite

Diverse structures facilitate direct exchange of proteins between cells, including plasmadesmata in plants and tunnelling nanotubes in bacteria and higher eukaryotes. Here we describe a new mechanism of protein transfer, flagellar membrane fusion, in the unicellular parasite Trypanosoma brucei. When fluorescently tagged trypanosomes were co-cultured, a small proportion of double-positive cells were observed. The formation of double-positive cells was dependent on the presence of extracellular calcium and was enhanced by placing cells in medium supplemented with fresh bovine serum. Time-lapse microscopy revealed that double-positive cells arose by bidirectional protein exchange in the absence of nuclear transfer. Furthermore, super-resolution microscopy showed that this process occurred in ≤1 minute, the limit of temporal resolution in these experiments. Both cytoplasmic and membrane proteins could be transferred provided they gained access to the flagellum. Intriguingly, a component of the RNAi machinery (Argonaute) was able to move between cells, raising the possibility that small interfering RNAs are transported as cargo. Transmission electron microscopy showed that shared flagella contained two axonemes and two paraflagellar rods bounded by a single membrane. In some cases flagellar fusion was partial and interactions between cells were transient. In other cases fusion occurred along the entire length of the flagellum, was stable for several hours and might be irreversible. Fusion did not appear to be deleterious for cell function: paired cells were motile and could give rise to progeny while fused. The motile flagella of unicellular organisms are related to the sensory cilia of higher eukaryotes, raising the possibility that protein transfer between cells via cilia or flagella occurs more widely in nature.

show abstract

Recent progress in research on cell-to-cell movement of rice viruses

Cited by 9 publications

References 88 publications

RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis

RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis

Repellency of essential oils against Nephotettix cincticeps: Laboratory and glasshouse assays

Flagellar membrane fusion and protein exchange in trypanosomes; a new form of cell-cell communication?

Contact Info

Product

Resources

About