Recent Progress in Proteins-Based Micelles as Drug Delivery Carriers

Mustafai, Aleena; Zubair, Muhammad; Hussain, Ajaz; Ullah, Aman

doi:10.3390/polym15040836

Cited by 15 publications

(4 citation statements)

References 125 publications

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“…The production of polymeric micelles may be accomplished by direct polymer dissolution in a solvent, solvent evaporation, and dialysis methods [57]. Micelle creation is affected by a number of factors, including the size of the hydrophobic chain on the amphiphilic molecule, the concentration of amphiphiles, the temperature, and the kind of solvent system [58].…”

Section: Polymeric Micellesmentioning

confidence: 99%

Recent Advances in Micro- and Nano-Drug Delivery Systems Based on Natural and Synthetic Biomaterials

Harun-Or-Rashid,

Aktar,

Hossain

et al. 2023

Polymers

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Polymeric drug delivery technology, which allows for medicinal ingredients to enter a cell more easily, has advanced considerably in recent decades. Innovative medication delivery strategies use biodegradable and bio-reducible polymers, and progress in the field has been accelerated by future possible research applications. Natural polymers utilized in polymeric drug delivery systems include arginine, chitosan, dextrin, polysaccharides, poly(glycolic acid), poly(lactic acid), and hyaluronic acid. Additionally, poly(2-hydroxyethyl methacrylate), poly(N-isopropyl acrylamide), poly(ethylenimine), dendritic polymers, biodegradable polymers, and bioabsorbable polymers as well as biomimetic and bio-related polymeric systems and drug-free macromolecular therapies have been employed in polymeric drug delivery. Different synthetic and natural biomaterials are in the clinical phase to mitigate different diseases. Drug delivery methods using natural and synthetic polymers are becoming increasingly common in the pharmaceutical industry, with biocompatible and bio-related copolymers and dendrimers having helped cure cancer as drug delivery systems. This review discusses all the above components and how, by combining synthetic and biological approaches, micro- and nano-drug delivery systems can result in revolutionary polymeric drug and gene delivery devices.

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Section: Polymeric Micellesmentioning

confidence: 99%

Recent Advances in Micro- and Nano-Drug Delivery Systems Based on Natural and Synthetic Biomaterials

Harun-Or-Rashid,

Aktar,

Hossain

et al. 2023

Polymers

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“…At different hydrophobic to hydrophilic block ratios, amphiphilic block copolymers self-assemble into micelles that have a hydrophobic core surrounded by a hydrophilic corona. [90,91] Micelles are easily assembled into monodisperse populations with inherently small sizes, typically below 100 nm, making them excellent candidates for therapeutics. Although polymeric micelles have demonstrated their potential as drug carriers in clinical applications, they are relatively underexplored as artificial organelles.…”

Section: Micellesmentioning

confidence: 99%

Synthetic Cells Revisited: Artificial Cell Construction Using Polymeric Building Blocks

Maffeis,

Heuberger,

Nikoletić

et al. 2023

Advanced Science

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The exponential growth of research on artificial cells and organelles underscores their potential as tools to advance the understanding of fundamental biological processes. The bottom–up construction from a variety of building blocks at the micro‐ and nanoscale, in combination with biomolecules is key to developing artificial cells. In this review, artificial cells are focused upon based on compartments where polymers are the main constituent of the assembly. Polymers are of particular interest due to their incredible chemical variety and the advantage of tuning the properties and functionality of their assemblies. First, the architectures of micro‐ and nanoscale polymer assemblies are introduced and then their usage as building blocks is elaborated upon. Different membrane‐bound and membrane‐less compartments and supramolecular structures and how they combine into advanced synthetic cells are presented. Then, the functional aspects are explored, addressing how artificial organelles in giant compartments mimic cellular processes. Finally, how artificial cells communicate with their surrounding and each other such as to adapt to an ever‐changing environment and achieve collective behavior as a steppingstone toward artificial tissues, is taken a look at. Engineering artificial cells with highly controllable and programmable features open new avenues for the development of sophisticated multifunctional systems.

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“…Nanoparticles, along with micelles, deserve special attention as promising drug carriers [33][34][35][36]. Polymeric micelles are promising carriers of a wide range of drugs, since they have a number of properties [37][38][39][40][41][42][43]: (1) the external hydrophilic shell ensures the colloidal stability of the system; (2) the internal hydrophobic core is necessary for the solubilization of drugs, which are often poorly soluble (which limits their use in medicine); (3) thermodynamic stability; (4) the possibility of obtaining biocompatible micellar structures; (5) increased permeability of the drug to target cells due to fatty acids; (6) wide possibilities for creating stimulus-sensitive delivery systems, for example, in tumors. For the latter, chitosan demonstrated pH sensitivity to a slightly acidic environment (tumors), and lipoic acid residues with S-S bonds between various polymer chains provided glutathione sensitivity [44].…”

Section: Introductionmentioning

confidence: 99%

Specific FRET Probes Sensitive to Chitosan-Based Polymeric Micelles Formation, Drug-Loading, and Fine Structural Features

Zlotnikov,

Savchenko,

Kudryashova

2024

Polymers

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Förster resonance energy transfer (FRET) probes are a promising tool for studying numerous biochemical processes. In this paper, we show the application of the FRET phenomenon to observe the micelle formation from surfactants, micelles self-assembling from chitosan grafted with fatty acid (oleic—OA, or lipoic—LA), cross-linking of SH groups in the micelle’s core, and inclusion and release of the model drug cargo from the micelles. Using the carbodiimide approach, amphiphilic chitosan-based polymers with (1) SH groups, (2) crosslinked with S-S between polymer chains, and (3) without SH and S-S groups were synthesized, followed by characterization by FTIR and NMR spectroscopy. Two pairs of fluorophores were investigated: 4-methylumbelliferon-trimethylammoniocinnamate—rhodamine (MUTMAC–R6G) and fluorescein isothiocyanate—rhodamine (FITC–R6G). While FITC–R6G has been described before as an FRET-producing pair, for MUTMAC–R6G, this has not been described. R6G, in addition to being an acceptor fluorophore, also serves as a model cytostatic drug in drug-release experiments. As one could expect, in aqueous solution, FRET effect was poor, but when exposed to the micelles, both MUTMAC–R6G and FITC–R6G yielded a pronounced FRET effect. Most likely, the formation of micelles is accompanied by the forced convergence of fluorophores in the hydrophobic micelle core by a donor-to-acceptor distance (r) significantly closer than in the aqueous buffer solution, which was reflected in the increase in the FRET efficiency (E). Therefore, r(E) could be used as analytical signal of the micelle formation, including critical micelle concentration (CMC) and critical pre-micelle concentration (CPMC), yielding values in good agreement with the literature for similar systems. We found that the r-function provides analytically valuable information about the nature and mechanism of micelle formation. S-S crosslinking between polymer chains makes the micelle more compact and stable in the normal physiological conditions, but loosens in the glutathione-rich tumor microenvironment, which is considered as an efficient approach in targeted drug delivery. Indeed, we found that R6G, as a model cytostatic agent, is released from micelles with initial rate of 5%/h in a normal tissue microenvironment, but in a tumor microenvironment model (10 mM glutathione), the release of R6G from S-S stitched polymeric micelles increased up to 24%/h. Drug-loading capacity differed substantially: from 75–80% for nonstitched polymeric micelles to ~90% for S-S stitched micelles. Therefore, appropriate FRET probes can provide comprehensive information about the micellar system, thus helping to fine-tune the drug delivery system.

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Recent Progress in Proteins-Based Micelles as Drug Delivery Carriers

Cited by 15 publications

References 125 publications

Recent Advances in Micro- and Nano-Drug Delivery Systems Based on Natural and Synthetic Biomaterials

Recent Advances in Micro- and Nano-Drug Delivery Systems Based on Natural and Synthetic Biomaterials

Synthetic Cells Revisited: Artificial Cell Construction Using Polymeric Building Blocks

Specific FRET Probes Sensitive to Chitosan-Based Polymeric Micelles Formation, Drug-Loading, and Fine Structural Features

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