Recent progress in metabolomics using ion mobility-mass spectrometry

Levy, Allison J.; Oranzi, Nicholas R.; Ahmadireskety, Atiye; Kemperman, Robin H. J.; Wei, Michael S.; Yost, Richard A.

doi:10.1016/j.trac.2019.05.001

Cited by 41 publications

(25 citation statements)

References 54 publications

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“…It is also important to note that TENGi MS is a DI metabolomics approach, and similar to other DI method, it lacks isobaric resolution. Coupling TENGi to IM-MS 68 would be a promising way to further increasing coverage, and be applied to even broader range of rare sample metabolomics studies.…”

Section: Discussionmentioning

confidence: 99%

Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples

Bouza

et al. 2020

Nat Commun

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The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. Samples tested with this approach include exhaled breath condensate collected from cystic fibrosis patients as well as in vitro-cultured human mesenchymal stromal cells. Both test samples are only available in minimum amounts. Experiments show that picoliter-volume spray pulses suffice to generate high-quality spectral fingerprints, which increase the information density produced per unit sample volume. This TENGi nanoESI strategy has the potential to fill in the gap in metabolomics where liquid chromatography-MS-based analyses cannot be applied. Our method opens up avenues for future investigations into understanding metabolic changes caused by diseases or external stimuli.

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Section: Discussionmentioning

confidence: 99%

Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples

Bouza

et al. 2020

Nat Commun

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“…To overcome these issues, researchers have developed bioinformatic tools that use fragmentation rules [26], predict metabolite CID-MS n spectra [27,28], or use deep neuronal networks to assign mass spectrometric features to metabolite groups or specific compounds [21]. Alternatively, CCS values from IMS experiments can be utilized to add an additional descriptor for structure annotation as reviewed by Harris et al as well as Yost and co-workers [29,30]. Another strategy to facilitate compound identification of metabolites and lipids is the development and use of new MS n strategies as detailed in the following.…”

Section: Mass Spectrometric Interrogation Of Metabolite and Lipid Structuresmentioning

confidence: 99%

Advanced tandem mass spectrometry in metabolomics and lipidomics—methods and applications

Heiles

2021

Anal Bioanal Chem

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Metabolomics and lipidomics are new drivers of the omics era as molecular signatures and selected analytes allow phenotypic characterization and serve as biomarkers, respectively. The growing capabilities of untargeted and targeted workflows, which primarily rely on mass spectrometric platforms, enable extensive charting or identification of bioactive metabolites and lipids. Structural annotation of these compounds is key in order to link specific molecular entities to defined biochemical functions or phenotypes. Tandem mass spectrometry (MS), first and foremost collision-induced dissociation (CID), is the method of choice to unveil structural details of metabolites and lipids. But CID fragment ions are often not sufficient to fully characterize analytes. Therefore, recent years have seen a surge in alternative tandem MS methodologies that aim to offer full structural characterization of metabolites and lipids. In this article, principles, capabilities, drawbacks, and first applications of these “advanced tandem mass spectrometry” strategies will be critically reviewed. This includes tandem MS methods that are based on electrons, photons, and ion/molecule, as well as ion/ion reactions, combining tandem MS with concepts from optical spectroscopy and making use of derivatization strategies. In the final sections of this review, the first applications of these methodologies in combination with liquid chromatography or mass spectrometry imaging are highlighted and future perspectives for research in metabolomics and lipidomics are discussed. Graphical abstract

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“…These limitations are related to the resolution of isomers that present similar retention times and m / z ratios [ 47 ]. These limitations seem to have been overcome in recent years thanks to an additional coupling of ion mobility spectrometry (IMS) [ 48 ]. IMS has become the most valuable analytical technique for the analysis of complex mixtures, because it allows the discrimination of species that are neither separated by chromatography nor by MS.…”

Section: Phytochemical Characterization Of Bioactive Compoundsmentioning

confidence: 99%

The Role of High-Resolution Analytical Techniques in the Development of Functional Foods

Fernández-Ochoa

Leyva-Jiménez²,

Cádiz-Gurrea

et al. 2021

IJMS

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The approaches based on high-resolution analytical techniques, such as nuclear magnetic resonance or mass spectrometry coupled to chromatographic techniques, have a determining role in several of the stages necessary for the development of functional foods. The analyses of botanical extracts rich in bioactive compounds is one of the fundamental steps in order to identify and quantify their phytochemical composition. However, the compounds characterized in the extracts are not always responsible for the bioactive properties because they generally undergo metabolic reactions before reaching the therapeutic targets. For this reason, analytical techniques are also applied to analyze biological samples to know the bioavailability, pharmacokinetics and/or metabolism of the compounds ingested by animal or human models in nutritional intervention studies. In addition, these studies have also been applied to determine changes of endogenous metabolites caused by prolonged intake of compounds with bioactive potential. This review aims to describe the main types and modes of application of high-resolution analytical techniques in all these steps for functional food development.

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Recent progress in metabolomics using ion mobility-mass spectrometry

Cited by 41 publications

References 54 publications

Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples

Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples

Advanced tandem mass spectrometry in metabolomics and lipidomics—methods and applications

The Role of High-Resolution Analytical Techniques in the Development of Functional Foods

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