Recent progress in histochemistry

Zuber, Christian; Taatjes, Douglas J.; Roth, Jürgen

doi:10.1007/s00418-007-0350-2

Cited by 1 publication

(1 citation statement)

References 258 publications

(220 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cells are probably metaplastic Clara cells. In normal lungs, Clara cells are cuboidal non-ciliated secretory cells associated with lung homeostasis [24], but exposure to ovalbumin in mice provokes hypertrophy of the cells, which acquire a mucous cell phenotype [21]. Comparison of SNA and PAS staining showed that the same columnar epithelial cells reacted with both agents.…”

Section: Discussionmentioning

confidence: 99%

Asthma induction in mice leads to appearance of α2–3- and α2–6-linked sialic acid residues in respiratory goblet-like cells

et al. 2008

View full text Add to dashboard Cite

Allergic asthmatic inflammation in mice was induced by sensitization with ovalbumin and lipopolysaccharide from Escherichia coli and visualized in the airways of asthmatic mice by spatial and temporal changes of carbohydrates containing sialic acid residues. Immunohistochemistry was used to demonstrate binding of lectins and antibodies that detect alpha2-3- and alpha2-6-linked sialic acid residues. After sensitization and challenge, the histology of the lung changed markedly, and goblet-like cells appeared, most likely caused by Clara cell metaplasia. Normal Clara cells showed no reaction after incubation with the sialic acid detecting agents, while the goblet-like cells expressed both alpha2-3- and alpha2-6-linked sialic acid residues in the asthmatic animals. The lectins but not the antibodies reacted with intestinal goblet cells. Instead, an antibody recognizing a disialoganglioside, stained large mononuclear cells in the submucosa, indicating a difference in sialylation between goblet cells in the intestine and goblet-like cells developed from Clara cells.

show abstract

Section: Discussionmentioning

confidence: 99%