Recent progress in DNA analysis by capillary electrophoresis

Righetti, Pier Giorgio; Gelfi, Cecilia; D’Acunto, Maria Rosa

doi:10.1002/1522-2683(200205)23:10<1361::aid-elps1361>3.0.co;2-j

Cited by 85 publications

(48 citation statements)

References 99 publications

(135 reference statements)

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“…Robert Weinberger is the author of this short and unpretentious historical overview of the development of this technology. 7) Recent progress in DNA analysis by capillary electrophoresis [105]. A well-balanced review by Righetti, Gelfi and D'Acunto.…”

Section: Discussion: An Annotated List Of Useful Articlesmentioning

confidence: 99%

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

et al. 2002

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Over the last two decades, the introduction of new methods such as pulsed-field gel electrophoresis and capillary array electrophoresis has made it possible to map and sequence entire genomes, including our own. The development of these experimental methods has been helped by the progress of theoretical and computational sciences, and the interactions between these three modi operandi of modern science are still pushing the limits of our technologies. We now see a clear trend towards proteomics and microfluidic (even nanofluidic!) devices. In this review, we take a look at the progress of the field over the last 3 years using the glasses of the theoretical scientist and focusing mostly on new ideas and concepts. About a dozen different subfields are discussed and reviewed. We conclude by giving a commented list of some of the best review articles published over the last 2-3 years.

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Section: Discussion: An Annotated List Of Useful Articlesmentioning

confidence: 99%

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

et al. 2002

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“…Such microfabricated regular sieving structures hold promise as an alternative to gels or capillaries to improve biomolecule separation speed and resolution. Until recently, the main focus has been placed on the separation of long DNA molecules ͑Chou et al, Lander et al, 2001͒, limitations in gel electrophoresis ͑Turmel et al, 1990Gygi et al, 2000;Lambert et al, 2005͒, and well-known DNA separation physics ͑Giddings, 1965de Gennes, 1979;Stellwagen et al, 1997;Righetti et al, 2002;Slater et al, 2003͒. The interest in separation techniques for smaller physiologically relevant biomolecules is increasing ͑Corthals et al, 1997; Anderson and Anderson, 1998;Rabilloud, 2002;Freire and Wheeler, 2006͒.…”

Section: Macromolecule Separation Mechanisms Using Nanometer-sizementioning

confidence: 99%

Transport phenomena in nanofluidics

2008

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The transport of fluid in and around nanometer-sized objects with at least one characteristic dimension below 100 nm enables the occurrence of phenomena that are impossible at bigger length scales. This research field was only recently termed nanofluidics, but it has deep roots in science and technology. Nanofluidics has experienced considerable growth in recent years, as is confirmed by significant scientific and practical achievements. This review focuses on the physical properties and operational mechanisms of the most common structures, such as nanometer-sized openings and nanowires in solution on a chip. Since the surface-to-volume ratio increases with miniaturization, this ratio is high in nanochannels, resulting in surface-charge-governed transport, which allows ion separation and is described by a comprehensive electrokinetic theory. The charge selectivity is most pronounced if the Debye screening length is comparable to the smallest dimension of the nanochannel cross section, leading to a predominantly counterion containing nanometer-sized aperture. These unique properties contribute to the charge-based partitioning of biomolecules at the microchannel-nanochannel interface. Additionally, at this free-energy barrier, size-based partitioning can be achieved when biomolecules and nanoconstrictions have similar dimensions. Furthermore, nanopores and nanowires are rooted in interesting physical concepts, and since these structures demonstrate sensitive, label-free, and real-time electrical detection of biomolecules, the technologies hold great promise for the life sciences. The purpose of this review is to describe physical mechanisms on the nanometer scale where new phenomena occur, in order to exploit these unique properties and realize integrated sample preparation and analysis systems.

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“…Tris is Good's buffers bind to the DNA not only by electro static interactions but also by hydrogen bonds primarily to the pyrimidine or purine rings [9]. Tris decrease the metal ion-catalyzed oxidation of Cys, consequently, Cys being available for interaction with the other reagent.…”

Section: Tris and Hepes Buffersmentioning

confidence: 99%

Important Factors Influencing Protein Crystallization

Abdalla¹

2016

Glob J Biotechnol Biomater Sci

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The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment, additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration. The aim of this paper to give very helpful advice for crystallization.

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Recent progress in DNA analysis by capillary electrophoresis

Cited by 85 publications

References 99 publications

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Transport phenomena in nanofluidics

Important Factors Influencing Protein Crystallization

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