Recent progress in copper nanocluster-based fluorescent probing: a review

Qing, Taiping; Zhang, Kaiwu; Qing, Zhihe; Wang, Xuan; Long, Caicheng; Zhang, Peng; Hai-zhi, HU; Feng, Bo

doi:10.1007/s00604-019-3747-4

Cited by 106 publications

(51 citation statements)

References 157 publications

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“…In the following we describe some sensing applications, and we do this according to the target species to be detected. More detailed description and application of CuNCs can be found in the reviews by Yi Lv, 85 Chuanxi Wang, 392 Bo Feng, 393 Baisong Chang 394 and Yuming Huang. 395 Ronghua Yang 396 and Erkang Wang 397 summarized the recent progress in biosensing applications of CuNCs utilizing DNA scaffolds.…”

Section: Applications In Sensingmentioning

confidence: 99%

Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications

2021

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Section: Applications In Sensingmentioning

confidence: 99%

Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications

2021

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“…Developments are rapidly advancing in both CRISPR-Cas and CuNP fluorescent biosensors [ 14 , 15 , 29 ]. Interestingly, in addition to enzymes and nucleic acids, these applications are beginning to include the detection of small molecules, such as uric acid [ 30 ], metals [ 31–33 ], iodide [ 34 ], biothiols [ 20 ] and dopamine [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…To overcome the limitations of FRET reporters, we coupled the CRISPR-Cas detection to an alternative reporter using fluorescent copper nanoparticles (CuNPs). CuNPs exhibit great potential for fluorescent bioassays as a result of their high fluorescence yield, good photostability and a visible emission (orange/red) when excited with UV-light [ 14–16 ]. For CuNPs to form, DNA has been used as a template to support the binding of copper ions and the subsequent chemical reduction.…”

Section: Introductionmentioning

confidence: 99%

Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles

Bogers

Berghuis

Busker

et al. 2020

Biology Methods and Protocols

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Fluorescence-based diagnostic tools are attractive and versatile tests with multiple advantages: ease of use, sensitivity and rapid results. The advent of CRISPR-Cas technology has created new avenues for the development of diagnostic testing tools. In the current study, by effectively combining the specific functions of two enzymes, CRISPR-Cas12a and terminal-deoxynucleotidyl-transferase (TdT), we developed a DNA detection assay that generates copper nanoparticles that are easily visible to the naked eye under UV-light; we named this detection assay CANTRIP: Cas12a Activated Nuclease poly-T Reporter Illuminating Particles. Upon specific target DNA recognition by Cas12a, single-stranded DNA (ssDNA) reporter oligos with blocked 3’-ends are cut into smaller ssDNA fragments, thereby generating neo 3’-OH moieties. TdT subsequently elongates these newly formed ssDNA fragments, incorporating only dTTP nucleotides, and these poly(thymine)-tails subsequently function as scaffolds for the formation of copper nanoparticles (CuNPs). These CuNPs produce a bright fluorescent signal upon UV excitation, and thus, this bright orange signal indicates the presence of target DNA, which in this proof-of-concept study consisted of anthrax lethal factor plasmid DNA. CANTRIP, which combines two detection platforms consisting of CRISPR-Cas12a and fluorescent copper-nanoparticles into a single reaction, appears to be a robust, low-cost and simple diagnostic tool.

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“…With the advent of technologies for NC synthesis and optimization, Cu NCs have been exploited in diagnosis and drug monitoring for different diseases, ranging from cancer to epilepsy ( Table 1 ). Diagnosis using Cu NCs is performed largely based on the detection of abnormal molecular events in the pathological site. In cancer cells, abnormal molecular events are usually manifested in terms of alternations in protein expression, changes in the level of reactive oxygen/nitrogen species, and alternations in intracellular pH .…”

Section: Working Principles Of Cu Ncs As Theranostic Agentsmentioning

confidence: 99%

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

2020

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Theranostics refers to the incorporation of therapeutic and diagnostic functions into one material system. An important class of nanomaterials exploited for theranostics is metal nanoclusters (NCs). In contrast to gold and silver NCs, copper is an essential trace element for humans. It can be more easily removed from the body. This, along with the low cost of copper that offers potential large‐scale nanotechnology applications, means that copper NCs have attracted great interest in recent years. The latest advances in the design, synthesis, surface engineering, and applications of copper NCs in disease diagnosis, monitoring, and treatment are reviewed. Strategies to control and enhance the emission of copper NCs are considered. With this synopsis of the up‐to‐date development of copper NCs as theranostic agents, it is hoped that insights and directions for translating current advances from the laboratory to the clinic can be further advanced and accelerated.

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Recent progress in copper nanocluster-based fluorescent probing: a review

Cited by 106 publications

References 157 publications

Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications

Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications

Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

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