Recent progress in applications of nanoparticles in fish medicine: A review

Shaalan, Mohamed; Saleh, Mona; El-Mahdy, Magdy; El-Matbouli, Mansour

doi:10.1016/j.nano.2015.11.005

Cited by 167 publications

(81 citation statements)

References 94 publications

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“…Concurrently, there is a need to minimize use of antibiotics in farming, to combat antibiotic resistance [3, 4]. Thus, there is a growing need for alternative antimicrobial therapeutics, and metal and metal oxide nanoparticles are being widely investigated for their potential medical applications, including their use in fish medicine [22]. …”

Section: Discussionmentioning

confidence: 99%

“…One of the recent strategies to combat microbes and multi-drug resistant bacteria is the application of metallic nanoparticles, which exhibit antimicrobial activities [19–22]. …”

Section: Introductionmentioning

confidence: 99%

“…Exact mechanisms that underlie the antibacterial actions of silver nanoparticles are not completely understood [34, 35]. However, modes of action have been suggested by different researchers [22, 33–36] and include the interaction of the silver nanoparticles with the bacterial cell wall, production of reactive oxygen species (ROS), interaction with DNA, and release of Ag + ions.…”

Section: Introductionmentioning

confidence: 99%

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In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens

et al. 2017

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BackgroundAntibiotic resistance is a global issue that threatens public health. The excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. Metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. The aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major fish pathogens and assess their safety in vitro. Silver nanoparticles were synthesized by chemical reduction and characterized with UV–Vis spectroscopy, transmission electron microscopy and zeta sizer. The concentrations of silver and zinc oxide nanoparticles were measured using inductively coupled plasma-mass spectrometry. Subsequently, silver and zinc oxide nanoparticles were tested for their antimicrobial activity against Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida, Edwardsiella ictaluri, Edwardsiella tarda, Francisella noatunensis subsp. orientalis, Yersinia ruckeri and Aphanomyces invadans and the minimum inhibitory concentrations were determined. MTT assay was performed on eel kidney cell line (EK-1) to determine the cell viability after incubation with nanoparticles. The interaction between silver nanoparticles and A. salmonicida was investigated by transmission electron microscopy.ResultsThe tested nanoparticles exhibited marked antimicrobial activity. Silver nanoparticles inhibited the growth of both A. salmonicida and A. invadans at a concentration of 17 µg/mL. Zinc oxide nanoparticles inhibited the growth of A. salmonicida, Y. ruckeri and A. invadans at concentrations of 15.75, 31.5 and 3.15 µg/mL respectively. Silver nanoparticles showed higher cell viability when compared to zinc oxide nanoparticles in the MTT assay. Transmission electron microscopy showed the attachment of silver nanoparticles to the bacterial membrane and disruption of its integrity.ConclusionsThis is the first study on inhibitory effects of silver and zinc oxide nanoparticles towards A. salmonicida and A. invadans. Moreover, zinc oxide nanoparticles inhibited the growth of Y. ruckeri. In low concentrations, silver nanoparticles were less cytotoxic than zinc oxide nanoparticles and represent an alternative antimicrobial compound against A. hydrophila, A. salmonicida and A. invadans.

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Section: Discussionmentioning

confidence: 99%

“…One of the recent strategies to combat microbes and multi-drug resistant bacteria is the application of metallic nanoparticles, which exhibit antimicrobial activities [19–22]. …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens

et al. 2017

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“…Although different NP vaccines have been used in aquaculture [18,19,52,53,54], few studies have evaluated their ability to release the antigens in vitro [55,56]. It is important that the ability of the NPs to release antigens in vitro be evaluated as this would give insight into their ability to induce a protective immune response in vaccinated fish.…”

Section: Discussionmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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The use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. Biodegradable polymeric nanoparticles (NPs) have shown potential to serve as antigen delivery systems for oral vaccines. In this study the recombinant outer membrane protein A (rOmpA) of Edwardsiella tarda was encapsulated in chitosan NPs (NP-rOmpA) and used for oral vaccination of Labeo fimbriatus. The rOmpA purity was 85%, nanodiameter <500 nm, encapsulation efficiency 60.6%, zeta potential +19.05 mV, and there was an in vitro release of 49% of encapsulated antigen within 48 h post incubation in phosphate-buffered saline. Empty NPs and a non-formulated, inactivated whole cell E. tarda (IWC-ET) vaccine were used as controls. Post-vaccination antibody levels were significantly (p = 0.0458) higher in the NP-rOmpA vaccinated fish (Mean OD450 = 2.430) than in fish vaccinated with inactivated whole cell E. tarda (IWC-ET) vaccine (Mean OD450 = 1.735), which corresponded with post-challenge survival proportions (PCSP) of 73.3% and 48.28% for the NP-rOmpA and IWC-ET groups, respectively. Serum samples from NP-rOmpA-vaccinated fish had a higher inhibition rate for E. tarda growth on tryptic soy agar (TSA) than the IWC-ET group. There was no significant difference (p = 0.989) in PCSPs between fish vaccinated with empty NPs and the unvaccinated control fish, while serum from both groups showed no detectable antibodies against E. tarda. Overall, these data show that the NP-rOmpA vaccine produced higher antibody levels and had superior protection over the IWC-ET vaccine, showing that encapsulating OmpA in chitosan NPs confer improved protection against E. tarda mortality in L. fimbriatus. There is a need to elucidate the possible adjuvant effects of chitosan NPs and the immunological mechanisms of protective immunity induced by OMPs administered orally to fish.

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“…Colloidal carriers based-formulations were already seen as promising platform for the delivery of biologically active compounds to cell fish [11]. In this context, the supply of antioxidant agents can significantly contribute to fish health, and by the use of colloidal carriers such active principles may be addressed to the immunocompetent HK leucocytes.…”

Section: Discussionmentioning

confidence: 99%

Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

Trapani

Tripodo

Mandracchia

et al. 2016

JCB

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Recent progress in applications of nanoparticles in fish medicine: A review

Cited by 167 publications

References 94 publications

In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens

In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

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