Recent progress in analytical capillary isotachophoresis (2018 - March 2022)

Malá, Zdena; Gebauer, Petr

doi:10.1016/j.chroma.2022.463337

Cited by 15 publications

(7 citation statements)

References 75 publications

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“…With the exception of bidirection CITP, conventional CITP operates in cationic or anionic mode, and within one experiment, it can separate either cations or anions. Recently, a wide review on the history and developments of ITP in the last 50 years was published [96], and the latest developments and applications of CITP were reported in another review [133]. Theory and microfluidic applications of ITP are presented in another comprehensive review [134].…”

Section: Separations By the Particular Ce Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Separations By the Particular Ce Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…Currently, the HPCE methods, CZE [112], CITP [107], CIEF [112], affinity capillary electrophoresis (ACE) [137], CEKC, and CEC [138] are recognized as highly efficient and highly sensitive separation methods with wide applicability for separation, analysis, and characterization of peptides, including the separation of complex peptide mixtures during peptide mapping of proteins [23–25]. Thanks to their different separation principles, they are appreciated as valuable counterparts and/or complements of various LC methods, such as (U)HPLC [139], nano‐LC [34], IEC [140], SEC [141], and HILIC [142] that are the dominating techniques for analysis and purification of peptides and proteins including the area of peptide mapping.…”

Section: Separation Of Peptides From Digested Proteins By Ce Methodsmentioning

confidence: 99%

“…To overcome this issue, various chemical and instrumental techniques have been developed and used also for peptide mapping. These techniques include analyte stacking by field amplification in the sample dissolved in water or in organic solvent [104, 105] or by the concentrating and focusing effect of ITP [106, 107], transient‐ITP [89, 108], transient pseudo‐ITP [109], dynamic pH junction [110, 111], CIEF [112], or by increasing the optical pathlengths by using bubble cell or Z‐cell or by derivatization of peptides by fluorescent labels for more sensitive LIF detection [113–115]. However, the most efficient and most frequently used preconcentration in CE peptide mapping is based on the on‐line coupling with SPE or SPME.…”

Section: Sample Preparationmentioning

confidence: 99%

Peptide mapping of proteins by capillary electromigration methods

Kašička

2022

J of Separation Science

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This review article provides a wide overview of important developments and applications of capillary electromigration methods in the area of peptide mapping of proteins in the period 1997-mid-2022, including review articles on this topic. It deals with all major aspects of peptide mapping by capillary electromigration methods: i) precleavage sample preparation involving purification, preconcentration, denaturation, reduction and alkylation of protein(s) to be analyzed, ii) generation of peptide fragments by off-line or on-line enzymatic and/or chemical cleavage of protein(s), iii) postcleavage preparation of the generated peptide mixture for capillary electromigration separation, iv) separation of the complex peptide mixtures by one-, two-and multidimensional capillary electromigration methods coupled with mass spectrometry detection, and v) a large application of peptide mapping for variable purposes, such as qualitative analysis of monoclonal antibodies and other protein biopharmaceuticals, monitoring of posttranslational modifications, determination of primary structure and investigation of function of proteins in biochemical and clinical research, characterization of proteins of variable origin as well as for protein and peptide identification in proteomic and peptidomic studies.

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Section: Ce‐mass Spectrometrymentioning

confidence: 99%

“…The concentrations of analyte ions in individual zones are adapted to the concentration of LE ions and the result of this process is that although the more abundant components of the mixture may be diluted, the analytes at the low concentration level will be concentrated and the concentration range of the complex mixture will be significantly reduced [175]. Malá and Gebauer [176,177] recently published a nice overview of the development of ITP from 1967 to 2022. Challenges associated with working with proteins include that their solubility is sometimes limited and the fact that they exhibit a wide range of pH and bufferdependent electrophoretic mobilities due to their different pIs and sizes.…”

Section: 34mentioning

confidence: 99%

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Maráková

Opetová

Tomašovský

2023

J of Separation Science

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Capillary electrophoresis is recognized as a valued separation technique for its high separation efficiency, low sample consumption, good economic and ecological aspects, reproducibility, and complementarity to traditional liquid chromatography techniques. Capillary electrophoresis experiments are generally performed utilizing optical detection, such as ultraviolet or fluorescence detectors. However, in order to provide structural information, capillary electrophoresis hyphenated to highly sensitive and selective mass spectrometry has been developed to overcome the limitations of optical detections. Capillary electrophoresis‐mass spectrometry is increasingly popular in protein analysis, including biopharmaceutical and biomedical research. It is frequently applied for the determination of physicochemical and biochemical parameters of proteins, offers excellent performance for in‐depth characterizations of biopharmaceuticals at various levels of analysis, and has been also already proven as a promising tool in biomarker discovery. In this review, we focus on the possibilities and limitations of capillary electrophoresis‐mass spectrometry for protein analysis at their intact level. Various capillary electrophoresis modes and capillary electrophoresis‐mass spectrometry interfaces, as well as approaches to prevent protein adsorption and to enhance sample loading capacity, are discussed and the recent (2018–March 2023) developments and applications in the field of biopharmaceutical and biomedical analysis are summarized.

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Recent progress in analytical capillary isotachophoresis (2018 - March 2022)

Cited by 15 publications

References 75 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Peptide mapping of proteins by capillary electromigration methods

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

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