Recent progress and challenges in microbial polyhydroxybutyrate (PHB) production from CO2 as a sustainable feedstock: A state-of-the-art review

Lee, Jiye; Park, Hyun June; Moon, Myounghoon; Min, Kyoungseon

doi:10.1016/j.biortech.2021.125616

Cited by 49 publications

(27 citation statements)

References 82 publications

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“…Typically, the final biodegradation products of PHB are carbon dioxide and water, which can be recycled and reused through photosynthesis. [108][109][110][111][112][113] At the same time, a lipase-catalyzed enzymatic degradation of PHB to a cyclic oligomer and then repolymerization back to PHB were also achieved. 114,115 A similar chemical recycling was also reported.…”

Section: Chemically Recyclable Polymer Derived From Four-membered Rin...mentioning

confidence: 99%

Chemically recyclable polymer materials: polymerization and depolymerization cycles

Wang

2022

Green Chem.

123

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Section: Chemically Recyclable Polymer Derived From Four-membered Rin...mentioning

confidence: 99%

Chemically recyclable polymer materials: polymerization and depolymerization cycles

Wang

2022

Green Chem.

123

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“…Even though E. coli is not a natural PHA producer, the heterogonous expression of phaCAB gene cluster from R. eutropha could efficiently boost carbon flux from pyruvate towards PHB synthesis. Therefore, intensive studies focusing on CO 2 fixation ( Lee et al, 2021 ), pathway engineering ( Chen and Jiang, 2017 ) and feeding solution design of fed-batch fermentation ( Yang et al, 2014 ) have been performed to generate enhanced production yield of PHB. Besides, E. coli is an ideal workhorse for studying the novel-type PHA synthesis, such as copolymers of 3HB and lactate, glycolic acid, 4-hydroxybutyrate, 5-hydroxyvalerate and other monomers with functional groups ( Scheel et al, 2021 ).…”

Section: Workhorses For Pha Productionmentioning

confidence: 99%

“…Recently, high production yield of P34HB with 4HB molar ratio from 5 mol% to 26 mol% has been achieved by recombinant H. bluephagenesis based on NGIB platform, which also demonstrated the success in scale-up production of low cost conducted in 5-to-200 m³ fermenters ( Ling et al, 2018 ). Notably, Lee et al (2021) used engineered Escherichia coli to synthesize aromatic polyester, P(3HB- co -D-phenylacetate), from tyrosine, of which the molar ratio of D-phenylacetate monomer reaches up to 47.7 mol% ( Yang et al, 2018 ). Moreover, tailor-made copolymers, as well as block copolymers, consisting of two, three and even more units could be easily obtained by designing the supplementation formula of target precursors and feeding strategy thereof ( Yu et al, 2020 ).…”

Section: Metabolic Pathways For Pha Synthesismentioning

confidence: 99%

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

Gao

Hao

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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With the rapid development of synthetic biology, a variety of biopolymers can be obtained by recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one with promising material properties, such as biodegradability and biocompatibility against the petrol-based plastics. This study reviews the recent studies focusing on the microbial synthesis of PHA, including chassis engineering, pathways engineering for various substrates utilization and PHA monomer synthesis, and PHA synthase modification. In particular, advances in metabolic engineering of dominant workhorses, for example Halomonas, Ralstonia eutropha, Escherichia coli and Pseudomonas, with outstanding PHA accumulation capability, were summarized and discussed, providing a full landscape of diverse PHA biosynthesis. Meanwhile, we also introduced the recent efforts focusing on structural analysis and mutagenesis of PHA synthase, which significantly determines the polymerization activity of varied monomer structures and PHA molecular weight. Besides, perspectives and solutions were thus proposed for achieving scale-up PHA of low cost with customized material property in the coming future.

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“…As a result, continuous research efforts are carried out worldwide for the development of more sustainable and environmentally friendly packaging materials derived from renewable feedstocks, including agro, microbial sources, and biomasses [ 3 ]. In this context, biopolymers synthesized via bacterial fermentation, like the ones from the polyhydroxyalkanoates (PHAs) family that includes poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) polymers, are considered promising candidates for replacing the fossil fuel-based polymeric materials and addressing the waste disposal issue [ 5 , 6 ]. These biopolymers exhibit several features similar to those of fossil fuel-based polymers and, additionally, can be easily degraded by the action of enzymes and living organisms, eliminating the need for disposal systems [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate) Nanocomposites with Cellulose Nanocrystals

et al. 2022

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Poly(3-hydroxybutyrate) (PHB) is one of the most promising substitutes for the petroleum-based polymers used in the packaging and biomedical fields due to its biodegradability, biocompatibility, good stiffness, and strength, along with its good gas-barrier properties. One route to overcome some of the PHB’s weaknesses, such as its slow crystallization, brittleness, modest thermal stability, and low melt strength is the addition of cellulose nanocrystals (CNCs) and the production of PHB/CNCs nanocomposites. Choosing the adequate processing technology for the fabrication of the PHB/CNCs nanocomposites and a suitable surface treatment for the CNCs are key factors in obtaining a good interfacial adhesion, superior thermal stability, and mechanical performances for the resulting nanocomposites. The information provided in this review related to the preparation routes, thermal, mechanical, and barrier properties of the PHB/CNCs nanocomposites may represent a starting point in finding new strategies to reduce the manufacturing costs or to design better technological solutions for the production of these materials at industrial scale. It is outlined in this review that the use of low-value biomass resources in the obtaining of both PHB and CNCs might be a safe track for a circular and bio-based economy. Undoubtedly, the PHB/CNCs nanocomposites will be an important part of a greener future in terms of successful replacement of the conventional plastic materials in many engineering and biomedical applications.

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Recent progress and challenges in microbial polyhydroxybutyrate (PHB) production from CO2 as a sustainable feedstock: A state-of-the-art review

Cited by 49 publications

References 82 publications

Chemically recyclable polymer materials: polymerization and depolymerization cycles

Chemically recyclable polymer materials: polymerization and depolymerization cycles

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

Poly(3-hydroxybutyrate) Nanocomposites with Cellulose Nanocrystals

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