Recent insights into mammalian natural and synthetic ex utero embryogenesis

Oldak, Bernardo; Aguilera-Castrejon, Alejandro; Hanna, Jacob H.

doi:10.1016/j.gde.2022.101988

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…Neurological and metabolic disorders stand to benefit greatly from future advances which will undoubtedly include translational applications with medical potential. Encouragingly, advances in ex utero [227][228][229] and in utero [230,231] experimental techniques and technologies promise to bring optogenetics to mammalian embryonic development, an important step toward this goal. Further, the genetically encoded devices described herein offer our best chance at supplementing our insufficient understanding of basic cell, developmental, and disease mechanisms which have hitherto remained inaccessible.…”

Section: Discussionmentioning

confidence: 99%

Opticool: Cutting-edge transgenic optical tools

Fenelon,

Krause,

Koromila

2024

PLoS Genet

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Only a few short decades have passed since the sequencing of GFP, yet the modern repertoire of transgenically encoded optical tools implies an exponential proliferation of ever improving constructions to interrogate the subcellular environment. A myriad of tags for labeling proteins, RNA, or DNA have arisen in the last few decades, facilitating unprecedented visualization of subcellular components and processes. Development of a broad array of modern genetically encoded sensors allows real-time, in vivo detection of molecule levels, pH, forces, enzyme activity, and other subcellular and extracellular phenomena in ever expanding contexts. Optogenetic, genetically encoded optically controlled manipulation systems have gained traction in the biological research community and facilitate single-cell, real-time modulation of protein function in vivo in ever broadening, novel applications. While this field continues to explosively expand, references are needed to assist scientists seeking to use and improve these transgenic devices in new and exciting ways to interrogate development and disease. In this review, we endeavor to highlight the state and trajectory of the field of in vivo transgenic optical tools.

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