Recent Insights and Novel Bioinformatics Tools to Understand the Role of MicroRNAs Binding to 5' Untranslated Region

Sacco, Letizia Da; Masotti, Andrea

doi:10.3390/ijms14010480

Cited by 88 publications

(58 citation statements)

References 61 publications

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“…miRNAs can not only bind to the 5 0 UTRs of their target genes [49], but also to the gene coding region [50]. Thus, miRNAs can bind to virtually any region in their target transcripts, greatly expanding the number of their potential targets.…”

Section: Non-coding Rnas-critical Regulators Of Gene Functionmentioning

confidence: 99%

Emerging roles of miR-210 and other non-coding RNAs in the hypoxic response

Huang

Zuo

2014

ABBS

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Hypoxia is a key component of the tumor microenvironment and represents a well-documented source of therapeutic failure in clinical oncology. Recent work has provided support for the idea that non-coding RNAs, and in particular, microRNAs, may play important roles in the adaptive response to low oxygen in tumors. Specifically, all published studies agree that the induction of microRNA-210 (miR-210) is a consistent feature of the hypoxic response in both normal and malignant cells. miR-210 is a robust target of hypoxia-inducible factors, and its overexpression has been detected in a variety of diseases with a hypoxic component, including most solid tumors. High levels of miR-210 have been linked to an in vivo hypoxic signature and to adverse prognosis in breast and pancreatic cancer patients. A wide variety of miR-210 targets have been identified, pointing to roles in mitochondrial metabolism, angiogenesis, DNA damage response, apoptosis, and cell survival. Such targets are suspected to affect the development of tumors in multiple ways; therefore, an increased knowledge about miR-210's functions may lead to novel diagnostic and therapeutic approaches in cancer.

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Section: Non-coding Rnas-critical Regulators Of Gene Functionmentioning

confidence: 99%

Emerging roles of miR-210 and other non-coding RNAs in the hypoxic response

Huang

Zuo

2014

ABBS

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“…The regulation process can occur by degradation or inhibition of translation of their target messenger RNAs through base pairing at 3' or 5' untranslated regions (1,2). miRNAs are involved in important biological processes such as cell differentiation, proliferation and death (3).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of mir-183 and mir-494 promotes proliferation and migration in human breast cancer cell lines

et al. 2017

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Abstract. Breast cancer (BC) is a leading cause of cancer-associated mortality in females worldwide. MicroRNAs (miRNAs or miRs), a type of non-coding RNA, have been reported to be important in the regulation of BC onset and progression. Several studies have implicated the role of miR-183 and miR-494 in different types of cancer. However, the biological functions of these miRNAs in BC remain largely unknown. In the present study, the expression of both miRNAs was assessed in the MDA-MB-231 and MDA-MB-468 BC cell lines. It was hypothesized that miR-183 and miR-494 serve an important role in regulating the expression of key genes associated with the metastatic phenotype of BC cells. To further understand their role, the expression of these miRNAs was restored in selected BC cell lines. Functional assays revealed that overexpression of miR-183 or miR-494 modulated the proliferation and migration of MDA-MB-231 and MDA-MB-468 cells in vitro. Additionally, retinoblastoma 1 (RB1) was identified to be a downstream target of both miRNAs by in silico analysis. Western blotting revealed that upregulation of miR-183 was associated with downregulation of RB1 protein in MDA-MB-231 cells. In conclusion, the present results support the hypothesis that miR-183 and miR-494 serve a pivotal role in BC metastasis, and that miR-183 may act as an oncogene by targeting RB1 protein in MDA-MB-231 cells.

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“…Studies of other transcription factors and more information about the regulation of silk protein biosynthesis by miRNAs are needed. Besides binding at the 3'UTR of target mRNAs, miRNAs can bind at the 5'UTR of target mRNAs and usually promote expression or translation of the target gene (da Sacco and Masotti, 2012). Identification of B. mori miRNAs that upregulate BmFib-L expression will be important in the study of the mechanisms of silk protein biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro

Chen

Fan

Wang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmomiR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR-0015 in 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-0001-SV40] and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 [ie1-egfp-pribmo-miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 [ie1-egfp-pri-bmomiR-0001-SV40] or pcDNA3.0 [ie1-egfp-pri-bmo-miR-0015-SV40] with pGL3.0 [A3-luc-Fib-L-3'UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro.

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Recent Insights and Novel Bioinformatics Tools to Understand the Role of MicroRNAs Binding to 5' Untranslated Region

Cited by 88 publications

References 61 publications

Emerging roles of miR-210 and other non-coding RNAs in the hypoxic response

Emerging roles of miR-210 and other non-coding RNAs in the hypoxic response

Overexpression of mir-183 and mir-494 promotes proliferation and migration in human breast cancer cell lines

bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro

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