On October 27, 2020, this report was posted as an MMWR Early Release on the MMWR website (https://www.cdc.gov/mmwr). Data on transmission of SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19), among college athletes are limited. In August 2020, the Chicago Department of Public Health (CDPH) was notified of a cluster of COVID-19 cases among a university's men's and women's soccer teams. CDPH initiated an investigation, interviewed members of both teams, and collated laboratory data to understand transmission of SARS-CoV-2 within the teams. Numerous social gatherings with limited mask use or social distancing preceded the outbreak. Transmission resulted in 17 laboratory-confirmed COVID-19 cases across both teams (n = 45), likely from a single source introduction of SARS-CoV-2 (based on whole genome sequencing) and subsequent transmission during multiple gatherings. Colleges and universities are at risk for COVID-19 outbreaks because of shared housing and social gatherings where recommended prevention guidance is not followed. Improved strategies to promote mask use and social distancing among college-aged adults need to be implemented, as well as periodic repeat testing to identify asymptomatic infections and prevent outbreaks among groups at increased risk for infection because of frequent exposure to close contacts in congregate settings on and off campus. Investigation and Results University A student athletes returned to campus* during June and were required to have two negative real-time reverse transcription-polymerase chain reaction (RT-PCR) SARS-CoV-2 tests before participating in any preseason training activities. Voluntary training sessions for both soccer teams began in July. In August, a member of the men's soccer team reported COVID-19-related symptoms to coaching staff members (Figure). The student reported attending several social gatherings with teammates in the preceding 14 days, including a birthday party and an unsanctioned soccer match between the men's and women's teams. Over the next 2 days, five other soccer players reported symptoms, and both teams were instructed to isolate or quarantine. † Specimens * University policy limited on-campus congregating and gatherings and required mask use in all settings (except personal residence), social distancing, and daily health screening. † The term "quarantine" is used to describe the 14-day period after a person who might have been exposed to COVID-19 stays away from others. The term "isolation" is used to describe the 10-day period when a person stays away from others after that person experiences symptom onset or receives a positive SARS-CoV-2 test result.