Recent History of Heme-Containing Proteins: Advances in Structure, Functions, and Reaction Intermediate Determination

Collins, Daniel P.; Dawson, John H.

doi:10.1016/b978-0-08-097774-4.00306-5

Cited by 8 publications

(6 citation statements)

References 300 publications

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“…For example, the coassembly of peptides with photosensitizer molecules has emerged for a number of applications, including light harvesting and photocatalysis, 433–436 DNA sensing, 437 biomimetic materials, 438–440 and drug delivery and anti-tumor therapy. 441–443 Porphyrins are macrocyclic tetrapyrrole photosensitizers found in biological catalytic and light-harvesting systems 444,445 that have been exploited in these types of multicomponent systems due to their inherent photophysical properties. 446,447 While the self-assembly of molecules consisting of a porphyrin conjugated to a peptidic component has been studied for various applications, 433,440,443,448,449 this section will focus on a the coassembly of low molecular weight peptides and porphyrins into hybrid supramolecular structures.…”

Section: Coassembly Of Low Molecular Weight Peptidesmentioning

confidence: 99%

Multicomponent peptide assemblies

2018

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Self-assembled peptide nanostructures have been increasingly exploited as functional materials for applications in biomedicine and energy. The emergent properties of these nanomaterials determine the applications for which they can be exploited. It has recently been appreciated that nanomaterials composed of multicomponent coassembled peptides often display unique emergent properties that have the potential to dramatically expand the functional utility of peptide-based materials. This review presents recent efforts in the development of multicomponent peptide assemblies. The discussion includes multicomponent assemblies derived from short low molecular weight peptides, peptide amphiphiles, coiled coil peptides, collagen, and β-sheet peptides. The design, structure, emergent properties, and applications for these multicomponent assemblies are presented in order to illustrate the potential of these formulations as sophisticated next-generation bio-inspired materials.

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Section: Coassembly Of Low Molecular Weight Peptidesmentioning

confidence: 99%

Multicomponent peptide assemblies

2018

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“…Amino Acid Residues Reference α-amylase Arg74, Trpl88, Tyrl90, Glu84 [43,44] peroxidase His170, Asp247, His42, Arg38, Phe41 [45] lipase Cys181, Ser152 [46,47] protease Asp30, His68, Ser255, Tyr195 [48] The two-way ANOVA confirmed statistical differences between the enzyme types (p = 0.02) and enzyme inactivation in wheat flour (p < 0.01). There was no statistically significant interaction between the inactivation of enzymes in native form and enzymes in flour (F(3, 24) = 1.83, p = 0.17).…”

Section: Enzymementioning

confidence: 91%

Enzyme Activity and Physiochemical Properties of Flour after Supercritical Carbon Dioxide Processing

Leitgeb

Knez

Podrepšek

2022

Foods

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The objectives of this study were to inactivate the enzymes α-amylase, lipase, protease, and peroxidase in flour with supercritical carbon dioxide (scCO2), and to optimize the enzymatic treatment conditions. Enzyme inactivation is important, due to the undesirability of certain flour enzymes that cause adverse reactions during storage as unpleasant rancidity of flour, and, at the same time, reduce the shelf life of flour. Therefore, crude enzymes and flour were initially exposed to scCO2 to determine the effect on specific enzyme activity under appropriate conditions. The activity of the unwanted enzymes lipase and peroxidase decreased under optimal process conditions of scCO2 exposure, lipase by 30%, and peroxidase by 12%, respectively. It was discovered that the inactivation of enzymes in wheat flour occurred, where, at the same time, this sustainable method allows the regulation of enzyme activity in the baking process. Afterwards, the effect of scCO2 on the physicochemical properties of flour, morphological changes on starch granules, and content of total lipids was studied. In scCO2-treated white wheat flour, the fat content decreased by 46.15 ± 0.5%, the grain structure was not damaged, and the bread as the final product had a lower specific surface volume. Therefore, this could be a promising technology for flour pretreatment, potentially impacting the prolonging of its shelf-life.

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“…Catalase primarily consists of two structural components, the α-helix and the β-sheets, bound together by a long wrapping loop. 34 When Cat (pH 7) was treated with a Au 3+ solution, the pH was decreased to 2.3. The α-helix structures were drastically reduced at the expense of the increased β-sheet contents from 15.2 to 25.3%, as there were no significant changes in other secondary structural components of the protein due to the treatment (Figure 1c and Table 1).…”

Section: Impact Of the Protein Secondary Structure On The Formation O...mentioning

confidence: 99%

Silk-Hydrogel-Immobilized Gold-Nanocluster-Seeded Catalase Protein as a Pre-Plasmonic Probe for Colorimetric Peroxide Sensing

Thungon,

Kundu,

Bachu

et al. 2024

ACS Appl. Opt. Mater.

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Protein-stabilized gold nanoclusters (AuNCs) are emerging as luminescent probes for various sensing applications. However, the technical difficulty of designing solid analytical platforms with these probes impedes their sensor applications. Herein, blue fluorescent (emission ∼ λ 460nm ) catalase protein (Cat)-AuNCs with peroxidase-mimicking activity were synthesized through an aurichlorohydric acid-led reaction process that concurrently released the heme prosthetic group from the protein matrix, turning off the catalase activity. A significant structural transition with a ∼1.9-fold increase in the β-sheets of the protein occurred during the process of seeding AuNCs of size ∼1.5 nm (dia) in protein molecules, as revealed from the respective circular dichroism and scanning electron microscopy studies. An atomic force microscopy study revealed the presence of AuNCs in the surface periphery of the catalase protein. Upon interaction with the substrate H 2 O 2 , these nanoclusters were destabilized and transformed into free plasmonic gold nanoparticles (AuNPs) with an average size of 10 nm through an internal aggregation process. The plasmonic signal (absorbance λ 520nm ) intensity of the released AuNPs was increased with the increasing concentration of H 2 O 2 , offering a linear detection range of 20−200 mM (R 2 = 0.99) and a limit of detection of 1 mM for the peroxide. The phenomenon was utilized to develop a colorimetric sensor by immobilizing Cat-AuNCs in silk fibroin hydrogels, which offered stability to the clusters for up to 3 months. The plasmonic response signal from the sensor surface could be captured as a red pixel intensity and color change (yes/no format) for the respective quantitative and qualitative detections of H 2 O 2 for diverse applications.

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Recent History of Heme-Containing Proteins: Advances in Structure, Functions, and Reaction Intermediate Determination

Cited by 8 publications

References 300 publications

Multicomponent peptide assemblies

Multicomponent peptide assemblies

Enzyme Activity and Physiochemical Properties of Flour after Supercritical Carbon Dioxide Processing

Silk-Hydrogel-Immobilized Gold-Nanocluster-Seeded Catalase Protein as a Pre-Plasmonic Probe for Colorimetric Peroxide Sensing

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