Recent High Performance Thin Layer Chromatographic Studies on Biomphalaria glabrata (Gastropoda)

Fried, Bernard; Reddy, Aditya

doi:10.3390/chromatography2010118

Cited by 2 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with other methods used in chromatography, such as gas chromatography (GC) and high-performance liquid chromatography (HPLC), TLC is the simplest and least expensive to perform [4,5]. At the same time, both TLC and HPTLC have a wide variety of application fields, such as biotechnology, chemistry, phytochemistry, the pharmaceutical industry, the food industry, cosmetics and many others [6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

High-Performance Thin-Layer Chromatography (HPTLC) Method for Identification of Meloxicam and Piroxicam

et al. 2022

View full text Add to dashboard Cite

Background: High-performance thin-layer chromatography (HPTLC) is an advantageous, modern analytical technique based on the principles of thin-layer chromatography (TLC), which provides essential benefits, such as improved sample application, better and faster separation, and less mobile phase usage. The aim of this work was to develop a simple and rapid HPTLC method that could be used for the identification of meloxicam and piroxicam. Methods: HPTLC. An analysis was carried out using silica gel 60 F254 glass TLC plates and as the mobile phase: hexane: ethyl acetate: glacial acetic acid, in a ratio of 65:30:5 v/v/v. For the standard solution preparation, ethanol was used. Front: 60 mm. Detection was performed at 366 nm. Results: The Rf value for meloxicam was 0.81 and the Rf value for piroxicam was 0.57. The proposed method can be used in the detection of the analyzed compounds in very low concentrations. It was established that the detection limit of meloxicam was 0.04 μg per band and that of piroxicam was 0.05 μg per band. It was also established that the quantitation limit of meloxicam was 0.12 μg per band and that of piroxicam was 0.15 μg per band. Conclusions: The proposed method is simple, sensitive, stable, cost effective, and eco-friendly. It could be used in research or for routine quality control purposes.

show abstract