Recent developments towards disposable screen-printed biosensors incorporating a carbon ink modified with the redox mediotor. Meldola's Blue

Wedge, R.; Pemberton, R. M.; Hart, John P.; Luxton, Richard

doi:10.1051/analusis:1999270570

Cited by 16 publications

(7 citation statements)

References 17 publications

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“…The screen-printed sensors consisted of a carbon working electrode, printed from C10903D14 ink and a Ag/AgCl combined counter/ reference electrode deposited onto poly(vinylchloride) (PVC). The configuration for these has been shown in detail elsewhere [18,19]. The electrode working area (9 mm 2 ) was defined by a thin strip of insulating tape (RS Components, Corby, UK).…”

Section: Apparatusmentioning

confidence: 99%

Voltammetric Determination of Urinary 1‐Hydroxypyrene Using Molecularly Imprinted Polymer‐Modified Screen‐Printed Carbon Electrodes

et al. 2005

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A two step non-competitive affinity method for the trace determination of 1-hydroxypyrene (1-OHP) using a disposable molecularly imprinted polymer (MIP) modified screen-printed carbon electrode (MIP-SPCE) has been developed. The MIP was synthesized according to a novel strategy, which is described, and is capable of rebinding the phenolic analyte, 1-hydroxypyrene (1-OHP), from high pH aqueous organic media, via ionic interactions. In the first step of our method 1-OHP was accumulated at the MIP-SPCE from 35% aqueous methanol containing 0.014 M NaOH and 0.14 M NaCl, at open circuit. In the second step, the resulting SPCE with accumulated 1-OHP was then transferred to fresh, clean phosphate buffered aqueous methanol, and subjected to cyclic voltammetry (CV) or differential pulse voltammetry (DPV). The latter technique proved to be more sensitive at detecting 1-OHP, with a limit of detection of 182 nM and a linear range to 125 mM on unmodified electrodes. The possible effects of interference by related phenolic compounds in the MIP-SPCE of 1-OHP were investigated. Finally the method was evaluated by carrying out 1-OHP determinations on spiked human urine samples; the recovery of 1-OHP was 79.4% and the coefficient of variation was found to be 7.7% (n ¼ 4) using a separate MIP-SPCE for each determination. Therefore, the performance data suggests that the method is reliable at the concentrations examined in this study. The method was found to be superior to the direct determination of 1-OHP in human urine by DPV alone, which was greatly affected by interference from uric acid.

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Section: Apparatusmentioning

confidence: 99%

Voltammetric Determination of Urinary 1‐Hydroxypyrene Using Molecularly Imprinted Polymer‐Modified Screen‐Printed Carbon Electrodes

et al. 2005

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“…The design and fabrication procedure for these electrodes have been described in the literature. 36,37 A platinum wire is used as an auxiliary electrode for completing threeelectrode systems. The working electrode of screen-printedsystem is modified with the electrodeposition of iridium oxide (prepared as follows).…”

Section: Apparatusmentioning

confidence: 99%

Disposable Amperometric Sensor for Neurotransmitters Based on Screen-Printed Electrodes Modified with a Thin Iridium Oxide Film

2005

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Adrenaline and dopamine are very important catecholamine neurotransmitters in the mammalian central nervous system. Catecholamine drugs are also used to treat hypertension, bronchial asthma and organic heart disease, and are used in cardiac surgery and myocardial infraction. [1][2][3][4] The determination of catecholamines and their metabolites in body fluids, such as urine, plasma and serum, is one of the most important ways to evaluate the activity of the sympathetic nervous system and support the diagnosis of many diseases in fields of clinical medicine. Several highperformance liquid chromatography (HPLC) methods with online purification have been described for use in the analysis of catecholamines in biological samples using either fluorescence detection, [5][6][7] on-line chemical derivatization followed by fluorescence detection, [8][9][10][11] capillary electrophoresis 12,13 or flow injection analysis. 14,15 These methods generally have good sensitivity and reproducibility, and can meet practical requirements, but require expensive instruments, a well-controlled experimental condition and profound sample making. Therefore, simple methods are expected and attention has been paid to them. Electrochemical detection offers several advantages, including remarkable sensitivity, inherent minituralization and portability, independence of the optical path length or sample turbidity, low cost, low power requirements and high compatibility with advanced micromachining technologies. Because of the simple procedure and high sensitivity of electroanalysis and the electroactivity of catecholamines, studying and measuring catecholamines with electrochemical methods were carried out. HPLC coupled with electrochemical detection has been used for simultaneously measuring catecholamines. [16][17][18] In most of these applications, the use of bare electrodes for the electrochemical detection of catecholamines has a number of limitations, such as low sensitivity and reproducibility, a slow electron-transfer reaction, low stability over a wide range of solution composition and a high overpotential, at which the electron transfer process occurs. In order to maintain the stability of the electrode responses and also to improve the analytical characteristics of the detection of substrates, a chemical modification or electrochemical pretreatments should be applied to the working electrode.Gold electrodes modified with a self-assembled monolayer of 3-mercaptopropionic acid, 19 a glassy carbon electrode modified with cobalt(II) hexacyanoferrate films, 20 a glassy carbon electrodes modified with multiwall carbon nanotubes, 21 Pt and GC electrode modified with electropolymerization of luminal, 22 a L-cysteine self-assembled monolayer modified gold electrode, 23 a glassy carbon electrode modified with C60-dimethyl(β-cyclodextrine)2, 24 and a polyisonicotinic acid modified glassy carbon electrode, 25 have been used for the determination of catecholamines. Unfortunately, most modified electrodes used for catecholamines detection have cer...

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“…This said, there are an increasing number of reports on biosensor formats that use NADH cofactordependent dehydrogenases coupled with the mediator Meldolas Blue (7-dimethylamino-2,3-benzophenoxazine). These biosensors have proved successful in detecting a number of key target analytes in food including, lactate, ethanol, ammonia and malate [15][16][17] since they require significantly lower operating potentials, reducing the influence of oxidisable interfering compounds (e.g., phenylpropanoids in fresh produce), which may adversely affect biosensor operating performance [8]. In addition, one desirable characteristic facilitating the development of a commercial biosensor is that of high output currents (R. Pittson, personal communication, Gwent Electronic Materials (GEM) Ltd., UK) resulting from the enzymesubstrate reaction.…”

Section: Introductionmentioning

confidence: 99%

A pyruvate dehydrogenase-based amperometric biosensor for assessing pungency in onions (Allium cepa L.)

Abayomi

Terry

2007

Sens. & Instrumen. Food Qual.

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A disposable prototype electrochemical biosensor was constructed using pyruvate dehydrogenase immobilised on mediated Meldolas Blue electrodes to determine pungency in onions (Allium cepa L.). The optimum operating potential was +50 mV vs. Ag/AgCl reference/counter electrode. The biosensor was able to differentiate between mild and pungent bulbs with pyruvate concentrations ranging between &4 and 8 mM in freshly extracted juices. Resolution was 0.5 mM and thus was comparable to the standard Schwimmer and Westonbased colorimetric assay currently employed by industry for determining pungency in macerated onion tissue.

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Recent developments towards disposable screen-printed biosensors incorporating a carbon ink modified with the redox mediotor. Meldola's Blue

Cited by 16 publications

References 17 publications

Voltammetric Determination of Urinary 1‐Hydroxypyrene Using Molecularly Imprinted Polymer‐Modified Screen‐Printed Carbon Electrodes

Voltammetric Determination of Urinary 1‐Hydroxypyrene Using Molecularly Imprinted Polymer‐Modified Screen‐Printed Carbon Electrodes

Disposable Amperometric Sensor for Neurotransmitters Based on Screen-Printed Electrodes Modified with a Thin Iridium Oxide Film

A pyruvate dehydrogenase-based amperometric biosensor for assessing pungency in onions (Allium cepa L.)

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