Recent Developments of Reverse Micellar Techniques for Lysozyme, Bovine Serum Albumin, and Bromelain Extraction

Chia, Shir Reen; Tang, Malcolm S.Y.; Chow, Yin Hui; Ooi, Chien Wei; Krishnamoorthy, Rambabu; Zhu, Linna; Show, Pau Loke

doi:10.1007/s12033-019-00200-7

Cited by 9 publications

(11 citation statements)

References 58 publications

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“…Dynamics of lysozyme activity in cow blood serum. Lysozyme plays an important function as a biomarker produced by the cow's body (Chia et al, 2019). The lysozyme activity (LA) of the blood serum of cows of the control Group 1 practically did not alter during the experiment period -21.0-22.5%.…”

Section: Indicators Of Immunity In Associated Mycotoxicosis Of Cowsmentioning

confidence: 99%

Indicators of Immunity in Associated Mycotoxicosis of Cows

Chekan¹,

Шкромада²,

Фотіна³

et al. 2022

Scientific Horizons

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The issue of cattle reproduction was and still is one of the main tasks in cattle breeding. Losses of farms from infertility of cows are quite significant and range within 3.19-5.41 per 1 day of infertility. Mycotoxins produced by fungi of the Fusarium family, namely deoxynivalenol (DON) and zearalenone (ZEN) adversely affect not only the functioning of all organs and systems of the cow’s body, but also produce an immunosedative effect. The purpose of this study was to establish the effect of the DON and ZEN complex on the main indicators of the immune response of cows and its correction in a comparative aspect using a feed additive based on zeolite and organic acids and recombinant α-, g-interferons. The study material was the blood of cows (serum and stabilized) sick with mycotoxicosis caused by the association of DON and ZEN. Methods used: photonephelometric using E. coli test culture, spontaneous rosette formation with sheep red blood cells according to M. Jondal, modified method of rosette formation according to M. Wansbrough-Jones, R. Limatibul’s method, simple radial immunodiffusion in gel according to G. Mancini, precipitation in a polyethylene glycol solution according to M. Digeon. Experimental studies were performed on black-spotted cows in farms of the Sumy Oblast. The dynamics of the immune indicators of cows during the development of mycotoxicosis and upon treatment with products zeolite-based, organic acids, and an aqueous solution of recombinant α-, g-interferons were studied. It was found that the indicator of bactericidal, lysozyme, complementary, and phagocytic activity of cow blood serum under treatment increased to the indicator inherent in healthy animals. The dynamics of immunoglobulins in the treatment with zeolite and organic acids and recombinant α-, g-interferons was investigated, and an increase to the level of intact cows was established. It was proved that the indicators of the immune response of cows upon using a feed additive based on zeolite and organic acids at a dose of 2.5 kg per tonne of fodder and a preparation based on an aqueous solution of recombinant α-, g-interferons at a dose of 3 ml per animal were significantly higher

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Section: Indicators Of Immunity In Associated Mycotoxicosis Of Cowsmentioning

confidence: 99%

Indicators of Immunity in Associated Mycotoxicosis of Cows

Chekan¹,

Шкромада²,

Фотіна³

et al. 2022

Scientific Horizons

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“…In the selection of reactors, it was found that a biofilm reactor (173 U/ml) improved the yield of lysozyme more than a suspension cell reactor (110 U/ml) ( Ercan and Demirci, 2016 ). In addition, the separation of lysozyme is also a technical challenge ( Chia et al, 2019 ). Lysozyme is an active protease.…”

Section: Future Directionsmentioning

confidence: 99%

Recent Insights Into the Prognostic and Therapeutic Applications of Lysozymes

et al. 2021

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Lysozymes are naturally occurring enzymes present in a variety of biological organisms, such as bacteria, fungi, and animal bodily secretions and tissues. It is also the main ingredient of many ethnomedicines. It is well known that lysozymes and lysozyme-like enzymes can be used as anti-bacterial agents by degrading bacterial cell wall peptidoglycan that leads to cell death, and can also inhibit fungi, yeasts, and viruses. In addition to its direct antimicrobial activity, lysozyme is also an important component of the innate immune system in most mammals. Increasing evidence has shown the immune-modulatory effects of lysozymes against infection and inflammation. More recently, studies have revealed the anti-cancer activities of lysozyme in multiple types of tumors, potentially through its immune-modulatory activities. In this review, we summarized the major functions and underlying mechanisms of lysozymes derived from animal and plant sources. We highlighted the therapeutic applications and recent advances of lysozymes in cancers, hypertension, and viral diseases, aiming toseeking alternative therapies for standard medical treatment bypassing side effects. We also evaluated the role of lysozyme as a promising cancer marker for prognosis to indicate the outcomes recurrence for patients.

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“…With a structure akin to human serum albumin, BSA comprises 585 amino acid residues arranged in three helical folding domains, granting it exceptional binding capabilities with ligands, metal ions, nanofillers, and polymeric chains . Its advantageous properties encompass antioxidant capabilities, biocompatibility, biodegradability, and nontoxicity, making it conducive toward retarding tumor growth on wound surfaces and fostering tissue formation for rapid healing . By skillfully entrapping nanomaterials into BSA molecules, nanocomposites with high stability in aqueous environments and superior mechanical strength can be produced .…”

Section: Introductionmentioning

confidence: 99%

“…During the inflammatory phase, hypoalbuminemia or insufficient dietary protein levels can lead to a reduced fibroblast count and hinder proteoglycan or collagen formation, adversely affecting the healing process. Addressing this nutritional deficiency is essential, as it triggers the release of hormones that stimulate tyrosine-kinase, DNA synthesis, and cell proliferation, regulating cell growth and collagen synthesis over the wound surface for accelerated recovery and remodeling . Being a biocompatible, biodegradable, and water-soluble polymer, poly(vinyl alcohol) (PVA) is commonly employed in conjunction with nanoparticles and other components to prepare nanocomposites due to its outstanding film-forming ability, tensile strength, flexibility, and adhesive properties .…”

Section: Introductionmentioning

confidence: 99%

In Vivo Wound Healing in Drosophila melanogaster and Mouse Models: Synergistic Effect of Bovine Serum Albumin and Graphene Quantum Dots

Purohit,

Biswal,

Mohapatra

et al. 2023

ACS Appl. Bio Mater.

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Recent Developments of Reverse Micellar Techniques for Lysozyme, Bovine Serum Albumin, and Bromelain Extraction

Cited by 9 publications

References 58 publications

Indicators of Immunity in Associated Mycotoxicosis of Cows

Indicators of Immunity in Associated Mycotoxicosis of Cows

Recent Insights Into the Prognostic and Therapeutic Applications of Lysozymes

In Vivo Wound Healing in Drosophila melanogaster and Mouse Models: Synergistic Effect of Bovine Serum Albumin and Graphene Quantum Dots

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