Recent Developments in the Use of Baculovirus Expression Vectors

Possee, Robert D.; Chambers, Adam; Graves, Leo P.; Aksular, Mine; King, Linda A.

doi:10.21775/cimb.034.215

Cited by 23 publications

(15 citation statements)

References 36 publications

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“…We have described recombinant sources of several S related polypeptides from two common expression systems, recombinant baculoviruses and E.coli and used the proteins expressed for the analysis of seroconversion and for epitope mapping. The particular properties of the insect cell system, yield, robustness and the ability to perform at scale are discussed elsewhere [23], similarly the use of the T7 system in E.coli [24]. Using a set of overlapping S fragments we demonstrated epitope mapping of monoclonal antibodies 3G9 and CR3022 to residues 300-400 of S. Interestingly, neither antibody reacted with the RBD itself in the western blot format used despite it encompassing the residues recognised.…”

Section: Discussionmentioning

confidence: 97%

Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping

Jegouic

Loureiro

Thom

et al. 2020

Preprint

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The newly emergent SARS-CoV-2 coronavirus is closely related to SARS-CoV which emerged in 2002. Studies on coronaviruses in general, and SARS in particular, have identified the virus spike protein (S) as being central to virus tropism, to the generation of a protective antibody response and to the unambiguous detection of past infections. As a result of this centrality SARS-CoV-2 S protein has a role in many aspects of research from vaccines to diagnostic tests. We describe a number of recombinant forms of SARS-CoV-2 S expressed in commonly available expression systems and their preliminary use in diagnostics and epitope mapping. These sources may find use in the current and future analysis of the virus and the Covid-19 disease it causes.

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Section: Discussionmentioning

confidence: 97%

Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping

Jegouic

Loureiro

Thom

et al. 2020

Preprint

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“…In particular, the identification of two main groups of applications for engineered baculoviruses is possible: (a) systems for the expression of recombinant proteins within eukaryotic contexts and (b) the use of recombinant virions in mammals for different goals. The former are generally called Baculovirus Expression Vector Systems or simply BEVS (O'Reilly et al 1994;Possee et al 2020) and comprise platforms composed of vectors for molecular cloning that enable modifications of the AcMNPV genome (many being based on bacmids) and susceptible insect cell lines plus their culture media or a whole insect as a biofactory (Martínez-Solís et al 2019). The latter group contains engineered baculoviruses applied to mammals and is recognized as BacMam technologies (Airenne et al 2013;Mansouri and Berger 2018;Ono et al 2018).…”

Section: Baculoviruses In Biotechnologymentioning

confidence: 99%

“…These developments have led to different regulatory considerations because in some instances baculoviruses can be only a contaminant, while in others they are the main ingredient of the products. In contrast to the use of this technology in the production of bioinsecticides (where OBs of many viral species and production systems are used in larvae), genomeengineered BVs of AcMNPV multiplied on the Sf9 or Sf21 insect cell lines, among others, are generally the bases of the BEVS and BacMam technologies (Kwang et al 2016;Possee et al 2020).…”

Section: Baculoviruses In Biotechnologymentioning

confidence: 99%

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Targovnik

Simonin

Callum

et al. 2021

Appl Microbiol Biotechnol

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Baculoviruses are insect pathogens widely used as biotechnological tools in different fields of life sciences and technologies. The particular biology of these entities (biosafety viruses 1; large circular double-stranded DNA genomes, infective per se; generally of narrow host range on insect larvae; many of the latter being pests in agriculture) and the availability of molecular-biology procedures (e.g., genetic engineering to edit their genomes) and cellular resources (availability of cell lines that grow under in vitro culture conditions) have enabled the application of baculoviruses as active ingredients in pest control, as systems for the expression of recombinant proteins (Baculovirus Expression Vector Systems-BEVS) and as viral vectors for gene delivery in mammals or to display antigenic proteins (Baculoviruses applied on mammals-BacMam). Accordingly, BEVS and BacMam technologies have been introduced in academia because of their availability as commercial systems and ease of use and have also reached the human pharmaceutical industry, as incomparable tools in the development of biological products such as diagnostic kits, vaccines, protein therapies, and-though still in the conceptual stage involving animal models-gene therapies. Among all the baculovirus species, the Autographa californica multiple nucleopolyhedrovirus has been the most highly exploited in the above utilities for the human-biotechnology field. This review highlights the main achievements (in their different stages of development) of the use of BEVS and BacMam technologies for the generation of products for infectious and noninfectious human diseases. Key points• Baculoviruses can assist as biotechnological tools in human health problems.• Vaccines and diagnosis reagents produced in the baculovirus platform are described.• The use of recombinant baculovirus for gene therapy-based treatment is reviewed.

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“…Some of these viruses are used as bioinsecticides for the control of agricultural pests [7][8][9]. Additionally, these viruses are used as foreign protein expression systems in vitro or in vivo and as delivery systems for therapeutic genes in mammalian cells [10,11]. Baculoviruses have been isolated from different orders of insects (Lepidoptera, Hymenoptera and Diptera) and have two infective morphologies: budded virus (BV) and occlusion derived virus (ODV) [12] (Figure 1).…”

Section: Actual Genomic Knowledge About the Family Baculoviridaementioning

confidence: 99%

Advances in the Bioinformatics Knowledge of mRNA Polyadenylation in Baculovirus Genes

Peros

Cerrudo

Pilloff

et al. 2020

Viruses

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Baculoviruses are a group of insect viruses with large circular dsDNA genomes exploited in numerous biotechnological applications, such as the biological control of agricultural pests, the expression of recombinant proteins or the gene delivery of therapeutic sequences in mammals, among others. Their genomes encode between 80 and 200 proteins, of which 38 are shared by all reported species. Thanks to multi-omic studies, there is remarkable information about the baculoviral proteome and the temporality in the virus gene expression. This allows some functional elements of the genome to be very well described, such as promoters and open reading frames. However, less information is available about the transcription termination signals and, consequently, there are still imprecisions about what are the limits of the transcriptional units present in the baculovirus genomes and how is the processing of the 3′ end of viral mRNA. Regarding to this, in this review we provide an update about the characteristics of DNA signals involved in this process and we contribute to their correct prediction through an exhaustive analysis that involves bibliography information, data mining, RNA structure and a comprehensive study of the core gene 3′ ends from 180 baculovirus genomes.

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Recent Developments in the Use of Baculovirus Expression Vectors

Cited by 23 publications

References 36 publications

Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping

Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Advances in the Bioinformatics Knowledge of mRNA Polyadenylation in Baculovirus Genes

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