Recent developments in Phos-tag electrophoresis for the analysis of phosphoproteins in proteomics

Hirano, Hisashi; Shirakawa, Jun

doi:10.1080/14789450.2022.2052850

Cited by 2 publications

(1 citation statement)

References 84 publications

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“…For example, Zhou et al summarized the achievement of bioanalysis of epigenetic modifiers, including DNA methylation and protein phosphorylation [26]. Advances in Phos-tag-based methodologies for the separation and detection of the phosphoproteome have been reviewed by Kinoshita's group and Shirakawa's group [27][28][29][30]. In this review, we aim to systematically summarize the development of biosensors with metal ion-phosphate chelation interaction for molecular recognition.…”

Section: Introductionmentioning

confidence: 99%

Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Hao

Dong

et al. 2023

Molecules

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Biosensors show promising prospects in the assays of various targets due to their advantages of high sensitivity, good selectivity and rapid response. Molecular recognition is a key event of biosensors, which usually involves the interaction of antigen–antibody, aptamer–target, lectin–sugar, boronic acid–diol, metal chelation and DNA hybridization. Metal ions or complexes can specifically recognize phosphate groups in peptides or proteins, obviating the use of biorecognition elements. In this review, we summarized the design and applications of biosensors with metal ion–phosphate chelation interaction for molecular recognition. The sensing techniques include electrochemistry, fluorescence, colorimetry and so on.

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