Recent developments in novel blue/green/red/NIR small fluorescent probes for in cellulo tracking of RNA/DNA G-quadruplexes

Pandith, Anup; Kumara, Guralamatta Siddappa Ravi; Seo, Young Jun

doi:10.1016/j.jphotochemrev.2019.08.001

Cited by 33 publications

(23 citation statements)

References 120 publications

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“…These limitations can be overcome by using small-molecule optical probes [22][23][24] . Most G4 DNA optical probes reported to date rely on a large enhancement in emission intensity upon binding G4 ('switch-on'), compared to binding duplex (ds) DNA 24 .…”

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confidence: 99%

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

et al. 2021

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Guanine rich regions of oligonucleotides fold into quadruple-stranded structures called G-quadruplexes (G4s). Increasing evidence suggests that these G4 structures form in vivo and play a crucial role in cellular processes. However, their direct observation in live cells remains a challenge. Here we demonstrate that a fluorescent probe (DAOTA-M2) in conjunction with fluorescence lifetime imaging microscopy (FLIM) can identify G4s within nuclei of live and fixed cells. We present a FLIM-based cellular assay to study the interaction of non-fluorescent small molecules with G4s and apply it to a wide range of drug candidates. We also demonstrate that DAOTA-M2 can be used to study G4 stability in live cells. Reduction of FancJ and RTEL1 expression in mammalian cells increases the DAOTA-M2 lifetime and therefore suggests an increased number of G4s in these cells, implying that FancJ and RTEL1 play a role in resolving G4 structures in cellulo.

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confidence: 99%

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

et al. 2021

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“…Sen and Gilbert were the first to show that the ratio of Na + and K + ions is critical for the DNA G-quadruplexes' formation, the so-called "sodium-potassium switch" [47]. These non-canonical structures occur in promoters of oncogenes, such as MYC, MYB, FOS, and ABL [48]. In addition, experimental data show that G-quadruplexes are more frequent in cancer cells in comparison with normal cells [49].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Changes in Endothelial Cells Triggered by Na,K-ATPase Inhibition: A Search for Upstream Na+i/K+i Sensitive Genes

Климанова

Sidorenko

Abramicheva

et al. 2020

IJMS

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Stimulus-dependent elevation of intracellular Ca2+ affects gene expression via well-documented calmodulin-mediated signaling pathways. Recently, we found that the addition of extra- and intracellular Ca2+ chelators increased, rather than decreased, the number of genes expressed, and that this is affected by the elevation of [Na+]i/[K+]i-ratio. This assumes the existence of a novel Na+i/K+i-mediated Ca2+i-independent mechanism of excitation-transcription coupling. To identify upstream Na+i/K+i-sensitive genes, we examined the kinetics of transcriptomic changes in human umbilical vein endothelial cells (HUVEC) subjected to Na,K-ATPase inhibition by ouabain or K+-free medium. According to our data, microRNAs, transcription factors, and proteins involved in immune response and inflammation might be considered as key components of Na+i/K+i-mediated excitation-transcription coupling. Special attention was focused on the FOS gene and the possible mechanism of transcription regulation via G-quadruplexes, non-canonical secondary structures of nucleic acids, whose stability depends on [Na+]i/[K+]i-ratio. Verification of the [Na+]i/[K+]i-sensitive transcription regulation mechanism should be continued in forthcoming studies.

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“…Fluorescent metal complexes have been applied in multiple areas of science and technology, including as sensors in different (bio)analytical applications and as cancer imaging probes [ 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. In this context, the fluorescent properties of some metal-based complexes that specifically recognize G4 structures make them useful also as probes for the detection and visualization of these DNA structures in cells.…”

Section: Introductionmentioning

confidence: 99%

Metal-Based G-Quadruplex Binders for Cancer Theranostics

et al. 2021

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The ability of fluorescent small molecules, such as metal complexes, to selectively recognize G-quadruplex (G4) structures has opened a route to develop new probes for the visualization of these DNA structures in cells. The main goal of this review is to update the most recent research efforts towards the development of novel cancer theranostic agents using this type of metal-based probes that specifically recognize G4 structures. This encompassed a comprehensive overview of the most significant progress in the field, namely based on complexes with Cu, Pt, and Ru that are among the most studied metals to obtain this class of molecules. It is also discussed the potential interest of obtaining G4-binders with medical radiometals (e.g., 99mTc, 111In, 64Cu, 195mPt) suitable for diagnostic and/or therapeutic applications within nuclear medicine modalities, in order to enable their theranostic potential.

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Recent developments in novel blue/green/red/NIR small fluorescent probes for in cellulo tracking of RNA/DNA G-quadruplexes

Cited by 33 publications

References 120 publications

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Transcriptomic Changes in Endothelial Cells Triggered by Na,K-ATPase Inhibition: A Search for Upstream Na+i/K+i Sensitive Genes

Metal-Based G-Quadruplex Binders for Cancer Theranostics

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