Recent developments in enzyme immobilization technology for high-throughput processing in food industries

Taheri‐Kafrani, Asghar; Kharazmi, Sara; Nasrollahzadeh, Mahmoud; Soozanipour, Asieh; Ejeian, Fatemeh; Etedali, Parisa; Mansouri-Tehrani, Hajar-Alsadat; Razmjou, Amir; Yek, Samaneh Mahmoudi‐Gom; Varma, Rajender S.

doi:10.1080/10408398.2020.1793726

Cited by 60 publications

(42 citation statements)

References 274 publications

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“…The reason for this might be that both enzymes' simultaneous action led to a competition for binding sites, creating a less obvious synergistic effect. In recent years, research on enzyme immobilization technology has been receiving more attention [33][34][35]. For example, Dal et al [36] and Tasgin et al [37] reported that the immobilized pectin lyase displayed excellent thermal stability and pH stability, increasing the enzyme operation range.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Thermophilic Mannanase and Synergistic Hydrolysis of Galactomannan Combined with Swollenin

Chen

et al. 2021

Catalysts

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Aspergillus fumigatus HBFH5 is a thermophilic fungus which can efficiently degrade lignocellulose and which produces a variety of glycoside hydrolase. In the present study, a novel β-mannanase gene (AfMan5A) was expressed in Pichia pastoris and characterized. AfMan5A is composed of 373 amino acids residues, and has a calculated molecular weight of 40 kDa. It has been observed that the amino acid sequence of AfMan5A showed 74.4% homology with the ManBK from Aspergillus niger. In addition, the recombined AfMan5A exhibited optimal hydrolytic activity at 60 °C and pH 6.0. It had no activity loss after incubation for 1h at 60 °C, while 65% of the initial activity was observed after 1 h at 70 °C. Additionally, it maintained about 80% of its activity in the pH range from 3.0 to 9.0. When carob bean gum was used as the substrate, the Km and Vmax values of AfMan5A were 0.21 ± 0.05 mg·mL−1 and 15.22 ± 0.33 U mg−1·min−1, respectively. AfMan5A and AfSwol showed a strong synergistic interaction on galactomannan degradation, increasing the reduction of the sugars by up to 31%. Therefore, these findings contribute to new strategies for improving the hydrolysis of galactomannan using the enzyme cocktail.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Thermophilic Mannanase and Synergistic Hydrolysis of Galactomannan Combined with Swollenin

Chen

et al. 2021

Catalysts

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“…The advanced techniques for enzyme or whole cell immobilization and other relevant frontier methodologies are also the major concerns of this Research Topic. Generally, enzyme or whole cell can be immobilized on/in the selected supports via principal routes of adsorption, covalent binding, cross-linking or encapsulation (Taheri-Kafrani et al, 2000). Following the general principles, nevertheless, a large number of cutting-edge technologies emerged in our Research Topic articles.…”

Section: Enzyme or Whole Cell Immobilization For Efficient Biocatalysmentioning

confidence: 99%

Editorial: Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques

Lou

Fernández‐Lucas

et al. 2021

Front. Bioeng. Biotechnol.

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“…Additionally, enzymes are renewables and can be recycled for multiple use when immobilized on suitable support material. [ 82–84 ] Acid hydrolysis, which typically has a faster reaction rate compared to enzymatic hydrolysis, is also used after LC pretreatment (Table 2). [ 65 ] The overall sugar yields of the acid hydrolysis of supercritical fluid pretreated biomass were varied in the range of 44.4–50.7 wt% of THS.…”

Section: Supercritical Carbon Dioxide Pretreatment Of Lignocellulosicmentioning

confidence: 99%

“…Extensive efforts have gone to overcome the drawbacks of the enzyme applications by immobilizing the enzyme on various support materials to recycle enzymes for their repeated application. [ 83,119–121 ] However, development of the immobilization support materials to be used in high pressure and SCFs based technologies is still largely unexplored.…”

Section: Enzyme‐assisted Supercritical Co2 For Hydrolysis Of Lignocelmentioning

confidence: 99%

Conversion of Lignocellulosic Biomass to Reducing Sugars in High Pressure and Supercritical Fluids: Greener Alternative for Biorefining of Renewables

Kumar

Kermanshahi-pour

Brar

et al. 2021

Advanced Sustainable Systems

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Supercritical fluids offer great potential to be employed in lignocellulosic biomass (LCB) fractionation in biorefinery. Supercritical carbon dioxide and water are greener alternatives compared with conventional reagents and have been investigated for the pretreatment and hydrolysis of lignocellulosic biomass. This review is focused on examining the fundamentals that govern the function of supercritical fluids in the pretreatment stage, as well as in the main hydrolysis reaction. Sub/supercritical carbon dioxide is used in pretreatment and sub/supercritical water has been the solvent of choice in hydrolysis of LCB. Significant research has gone into understanding the effect of process parameters such as temperature, pressure, cosolvent, and use of external catalyst on the sugar yield in biorefining of the LCB in supercritical fluids. It is shown that processes with reduced environmental impact and energy consumption can significantly enhance biorefining of LCB at commercial scale. Enzymatic hydrolysis of LCB in supercritical carbon dioxide is a promising approach that can accommodate mild reaction conditions. Developing an understanding of the performance of enzymes in high pressure systems and designing carriers for enzyme immobilization and further recycling is expected to enable one pot pretreatment and hydrolysis and is an important milestone in processing renewable resources for deriving biofuel and value‐added chemicals.

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Recent developments in enzyme immobilization technology for high-throughput processing in food industries

Cited by 60 publications

References 274 publications

Characterization of a Novel Thermophilic Mannanase and Synergistic Hydrolysis of Galactomannan Combined with Swollenin

Characterization of a Novel Thermophilic Mannanase and Synergistic Hydrolysis of Galactomannan Combined with Swollenin

Editorial: Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques

Conversion of Lignocellulosic Biomass to Reducing Sugars in High Pressure and Supercritical Fluids: Greener Alternative for Biorefining of Renewables

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