Recent developments in analytical methodology for 8-hydroxy-2′-deoxyguanosine and related compounds

Peoples, Michael C.; Karnes, H. Thomas

doi:10.1016/j.jchromb.2005.10.001

Cited by 41 publications

(39 citation statements)

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“…8-OxodG is one of the most widely used biomarkers of oxidative stress, mainly because of its abundance in DNA and also (Karihtala and Soini, 2007). Compared with other bases, guanine most readily undergoes an oxidative attack in the presence of ROS (Peoples and Karnes, 2005). 8-OxodG can induce GC-TA transversion mutations particularly during DNA replication and thus, if these oxidative lesions are not repaired, they can become mutagenic (Peoples and Karnes, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with other bases, guanine most readily undergoes an oxidative attack in the presence of ROS (Peoples and Karnes, 2005). 8-OxodG can induce GC-TA transversion mutations particularly during DNA replication and thus, if these oxidative lesions are not repaired, they can become mutagenic (Peoples and Karnes, 2005). There is a rather broad consensus that extracellular 8-oxodG levels are not affected by diet, cell death or artefactual formation (Cooke et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

et al. 2010

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BACKGROUND: is the commonly used marker of oxidative stress-derived DNA damage. 8-OxodG formation is regulated by local antioxidant capacity and DNA repair enzyme activity. Earlier studies have reported contradictory data on the function of 8-oxodG as a prognostic factor in different cancer types. METHODS: We assessed pre-operative serum 8-oxodG levels with an enzyme-linked immunosorbent assay in a well-defined series of 173 breast cancer patients. 8-OxodG expression in the nuclei of cancer cells from 150 of these patients was examined by immunohistochemistry. RESULTS: The serum 8-oxodG levels and immunohistochemical 8-oxodG expression were in concordance with each other (Po0.05). Negative 8-oxodG immunostaining was an independent prognostic factor for poor breast cancer-specific survival according to the multivariate analysis (Po0.01). This observation was even more remarkable when ductal carcinomas only (n ¼ 140) were considered (Po0.001). A low serum 8-oxodG level was associated statistically significantly with lymphatic vessel invasion and a positive lymph node status. CONCLUSIONS: Low serum 8-oxodG levels and a low immunohistochemical 8-oxodG expression were associated with an aggressive breast cancer phenotype. In addition, negative 8-oxodG immunostaining was a powerful prognostic factor for breast cancer-specific death in breast carcinoma patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

et al. 2010

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“…Previous analysis has concentrated on simplifying sample preparation and clean up techniques to reduce this artifactual oxidation. [31]. The decreased analysis time of this protocol may help to reduce artifactual oxidation by optimising the chromatographic parameters themselves.…”

Section: Discussionmentioning

confidence: 99%

Separation of oxidatively damaged DNA nucleobases and nucleosides on packed and monolith C18 columns by HPLC-UV-EC

Kelly

White

Smyth

2008

Journal of Chromatography B

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This study involves the incorporation of a commercially available Phenomenex Onyx C18 monolith column into the separation and detection of oxidative DNA damage. It includes thorough investigation of monolith performance and a comparison of the performance of monolith columns with a commercially available packed Restek reverse phase Ultra C18 column for the separation of DNA bases and nucleosides. The performance of the monolith was examined using efficiency, resolution, plate height, asymmetry and retention times, and in each case showed improved or at least comparable results in the separation of a mix of DNA bases and nucleosides. A 90% reduction, from just under 40 min. to just under 4 min., was obtained in the elution time of this separation. To the best of our knowledge, this is the first report of a fast monolith column separation successfully coupled to both a UV-vis and EC detector, which is especially useful for analysis of oxidative DNA damage. The determination of 8-oxoG and 8-OH-dG, oxidation products of guanine and 2'-deoxyguanosine, respectively, may be compromised by their ease of oxidation and therefore the fast separation, selective and sensitive detection, with no artifactual oxidation, detailed in this report, is ideal.

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“…12,13 Oxidative DNA damage products in urine are generally measured by high-power liquid chromatography (HPLC) methods. 14,15 Otherwise, the single-cell gel assay (also termed comet assay) is used to measure DNA damage levels of tissue. This is a sensitive method for detecting DNA strand breaks (including those created by alkali-labile sites) at the level of individual cell.…”

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confidence: 99%

Effects of Embryonic Neural Stem Cell Therapy on DNA Damage Products in Urine and Tissue After Spinal Cord Injury in Rats

Özgönül¹,

Konyalιoğlu²,

Dağcı³

et al. 2012

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : We tested the hypothesis that embryonic neural stem cell (ENSC) therapy reduces neuronal DNA damage in the lesional spinal cord (SC) region and assessed the significance of some DNA breakdown products in urine and tissue samples as biomarkers of efficiency of the ENSC therapy. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : DNA damage was measured by single cell gel electrophoresis (SCGE/Comet Assay) in spinal cord tissue of rats 7 (acute group) and 28 (chronic group) days after surgical resections of a 10 mm segment of the cord below T8-T9 and sham operations. As biomarkers of DNA damage, urinary 5-(hydroxymethyl) uracil (5HMU) and 2'-deoxyuridine (2dU) were analyzed by high-performance liquid chromatography (HPLC) with electrochemical detection (ED). R Re es su ul lt ts s: : Comet scores of the lesioned tissues were significantly higher in the acute and chronic spinal cord injury (SCI) compared to the sham operated groups (p=0.009 and p=0.009, respectively). After the ENSC transplantation, tail %, tail length and tail moment (T%, TL and TM, respectively) decreased in acute (p=0.009, p=0.009 and p=0.009, respectively) and chronically (p=0.009, p=0.009, p=0.009 respectively) injured rats. 5HMU and 2dU were significantly higher in the acute and the chronic groups than in the sham operated groups (p=0.009 and p=0.009, respectively). 5HMU concentration in the acute SCI group had a stronger correlation strongly with tail moment (r=0.9039, p=0.02). In the chronic injury group, 5HMU and 2dU concentrations correlated significantly with tail length (r=0.9990, p=0.001 and r=0.9272, p=0.016, respectively). While 5HMU seems to be a better biomarker for acute injury, both HMU and 2dU excretions increase in chronic damage. Besides, neurological functions of all the study animals were measured with the Basso-Beattie-Bresnahan (BBB) score. C Co on nc cl lu us si io on n: : Our results demonstrated that ENSC therapy of the lesioned tissue significantly decreased the levels of DNA breakdown products in urine and tissue samples of rats with acute and chronic SCI. Assessment of neurological recovery with the BBB test confirmed these findings. K Ke ey y W Wo or rd ds s: : Spinal cord injuries; comet assay; chromatography, high pressure liquid; 2'-deoxyuridylic acid; 5 hydroxymethyluracil Ö ÖZ ZE ET T A Am ma aç ç: : Embriyonik nöral kök hücre (ENKH) tedavisinin bozulan omurilik bölgesinde sinirsel DNA hasarını azalttığı hipotezini test ettik ve ENKH tedavisinin etkinliğini yansıtmak üzere idrar ve doku örneklerinde bazı DNA yıkım ürünlerinin önemini değerlendirdik. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : DNA hasar düzeyleri, 7 (akut grup) ve 28 (kronik grup) günlük travmalı sıçanların ve kontrol sıçanlarının T8-T9 omurilik bölgesinin cerrahi rezeksiyonu ile elde edilen 10 mm'lik bir segment dokusunda tek hücre jel elektroforez tekniği (THJE/Comet Testi) kullanılmak suretiyle öl-çüldü. İdrarda DNA hasarının biyolojik göstergeleri olarak, 5-hidroksimeti...

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Recent developments in analytical methodology for 8-hydroxy-2′-deoxyguanosine and related compounds

Cited by 41 publications

References 60 publications

8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

8-Hydroxydeoxyguanosine: a new potential independent prognostic factor in breast cancer

Separation of oxidatively damaged DNA nucleobases and nucleosides on packed and monolith C18 columns by HPLC-UV-EC

Effects of Embryonic Neural Stem Cell Therapy on DNA Damage Products in Urine and Tissue After Spinal Cord Injury in Rats

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