Recent developments and applications of polymer monolithic stationary phases

Eeltink, Sebastiaan; Meston, Daniel; Švec, František

doi:10.1002/ansa.202100006

Cited by 7 publications

(5 citation statements)

References 84 publications

(162 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, the mass transfer of analytes in organic monoliths is dominantly realized by convection, while diffusion plays a marginal role. This feature predetermines their dominant utilization in the fast separation of slowly diffusing analytes at higher mobile phase velocities. − …”

Section: Rplc Methods For Bottom-up Proteomic Analysesmentioning

confidence: 99%

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial

et al. 2022

View full text Add to dashboard Cite

The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry. It is thus not surprising that the MS instrument attracts the most attention during LC-MS method development, whereas optimizing conditions for peptide separation using reversed-phase liquid chromatography (RPLC) remains somewhat in its shadow. Consequently, the wisdom of the fundaments of chromatography is slowly vanishing from some laboratories. However, the full potential of advanced MS instruments cannot be achieved without highly efficient RPLC. This is impossible to attain without understanding fundamental processes in the chromatographic system and the properties of peptides important for their chromatographic behavior. We wrote this tutorial intending to give practitioners an overview of critical aspects of peptide separation using RPLC to facilitate setting the LC parameters so that they can leverage the full capabilities of their MS instruments. After briefly introducing the gradient separation of peptides, we discuss their properties that affect the quality of LC-MS chromatograms the most. Next, we address the in-column and extra-column broadening. The last section is devoted to key parameters of LC-MS methods. We also extracted trends in practice from recent bottom-up proteomics studies and correlated them with the current knowledge on peptide RPLC separation.

show abstract

Section: Rplc Methods For Bottom-up Proteomic Analysesmentioning

confidence: 99%

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, tuning monolithic morphology is complex and ensuring specicity of monoliths for sample enrichment is not always consistent. 146 Aptamers can also be used for partitioning in microuidic devices, which offer advantages including rapid analysis, decreased sample and chemical consumption, and portability. Cho et al developed a microuidic chip with immobilized RNA aptamer on beads to detect hepatitis C virus (HCV) RNA polymerase from serum.…”

Section: Optimization Of Aptamer-based Affinity Chromatography Platformmentioning

confidence: 99%

“…However, tuning monolithic morphology is complex and ensuring specificity of monoliths for sample enrichment is not always consistent. 146 …”

Section: Separationsmentioning

confidence: 99%

Aptamers 101: aptamer discovery andin vitroapplications in biosensors and separations

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Unfortunately, the selection of cross-linking monomers is quite limited. The family of applied cross-linkers mainly includes divinylbenzene, ethylene glikol dimethacrylate, N , N ′ methylenebisacryloamide, and trimethylolpropane trimethacrylate [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. The cross-linking monomer concentration in the polymerization mixture can vary to a great extent.…”

Section: Introductionmentioning

confidence: 99%

Influence of the Polymerization Parameters on the Porosity and Thermal Stability of Polymeric Monoliths

Maciejewska

2024

Materials

View full text Add to dashboard Cite

Rigid porous polymeric monoliths are robust, highly efficient, versatile stationary phases. They offer simple preparation and convenient modification provided by a whole range of synthesis factors, e.g., starting monomers, cross-linkers, initiators, porogens, polymerization techniques, and temperature. The main aim of this study was to synthesize polymeric monoliths and determine the correlation between polymerization parameters and the porosity and thermal stability of the obtained materials. Polymeric monoliths were synthesized directly in HPLC columns using N-vinyl-2-pyrrolidone (NVP) and 4-vinylpiridine (4VP) as functional monomers, with trimethylolpropane trimethacrylate (TRIM) serving as the cross-linking monomer. During copolymerization a mixture of cyclohexanol/decane-1-ol was used as the pore-forming diluent. Polymerization was carried out at two different temperatures: 55 and 75 °C. As a result, monoliths with highly developed internal structure were synthesized. The value of their specific surface area was in the range of 92 m2/g to 598 m2/g, depending on the monomer composition and polymerization temperature. Thermal properties of the obtained materials were investigated by means of thermogravimetry (TG). Significant differences in thermal behavior were noticed between monoliths synthesized at 55 and 75 °C. Additionally, the poly(NVP-co-TRIM) monolith was successfully applied in GC analyses.

show abstract

Recent developments and applications of polymer monolithic stationary phases

Cited by 7 publications

References 84 publications

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial

Aptamers 101: aptamer discovery andin vitroapplications in biosensors and separations

Influence of the Polymerization Parameters on the Porosity and Thermal Stability of Polymeric Monoliths

Contact Info

Product

Resources

About