Recent Development of Sandwich Assay Based on the Nanobiotechnologies for Proteins, Nucleic Acids, Small Molecules, and Ions

Shen, Ji; Li, Yuebin; Gu, Haoshuang; Xia, Fan; Zuo, Xiaolei

doi:10.1021/cr300248x

Cited by 234 publications

(144 citation statements)

References 429 publications

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“…Many studies have been focused on adopting chromatic methods for detecting DNA, proteins, small molecules and ions [2][3][4]. For example, Lee et al used the aggregation of carbon nanotubes (CNTs) to detect nucleic acid and achieved the detection limit of 10 -12 M [5].…”

Section: Introductionmentioning

confidence: 99%

A Readable Chromatic Biosensor with a Tunable Detection Threshold

Tsai¹,

Hong²,

Huang³

et al. 2016

J Microb Biochem Technol

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Section: Introductionmentioning

confidence: 99%

A Readable Chromatic Biosensor with a Tunable Detection Threshold

Tsai¹,

Hong²,

Huang³

et al. 2016

J Microb Biochem Technol

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“…Recent advances in material chemistry, especially with introduction of various nanomaterials, together with launching of novel transducing protocols and biorecognition elements are behind a huge development in ultrasensitive and multiplexed analysis of a wide range of disease biomarkers [18][19][20][21][22][23]. It is well-known that presence of labels can negatively affect binding [24,25] and thus label-free methods of analysis are preferential option for diagnostic purposes.…”

Section: Introductionmentioning

confidence: 99%

Glycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods

Kluková

Bertók

Petrikova

et al. 2015

Analytica Chimica Acta

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Systemic sclerosis (SSc) is an autoimmune disease seriously affecting patient´s quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.

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“…However, their excellent performance is achieved at the expenses of expensive instruments, complicated manipulations and time-consuming sample preparation processes. Over the past decades, with the advancement of various nanotechnologies, versatile biochemical sensors based on nanomaterials have been developed for Hg(II) ion detection [7][8][9][10][11]. Among them, gold nanoparticle (AuNP) is one of the most adopted probes to provide an alternative to conventional detection methods [12,13].…”

Section: Introductionmentioning

confidence: 99%

A Concentration-Controllable Microfluidic Droplet Mixer for Mercury Ion Detection

et al. 2015

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Abstract:A microfluidic droplet mixer is developed for rapid detection of Hg(II) ions. Reagent concentration and droplets can be precisely controlled by adjusting the flow rates of different fluid phases. By selecting suitable flow rates of the oil phase, probe phase and sample phase, probe droplets and sample droplets can be matched and merged in pairs and subsequently well-mixed in the poly (dimethylsiloxane) (PDMS) channels. The fluorescence enhancement probe (Rhodamine B mixed with gold nanoparticles) encapsulated in droplets can react with Hg(II) ions. The Hg(II) ion concentration in the sample droplets is adjusted from about 0 to 1000 nM through fluid regulation to simulate possible various contaminative water samples. The intensity of the emission fluorescence is sensitive to Hg(II) ions (increases as the Hg(II) ion concentration increases). Through the analysis of the acquired fluorescence images, the concentration of Hg(II) ions can be precisely detected. With the advantages of less time, cost consumption and easier manipulations, this device would have a great potential in micro-scale sample assays and real-time chemical reaction studies.

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Recent Development of Sandwich Assay Based on the Nanobiotechnologies for Proteins, Nucleic Acids, Small Molecules, and Ions

Cited by 234 publications

References 429 publications

A Readable Chromatic Biosensor with a Tunable Detection Threshold

A Readable Chromatic Biosensor with a Tunable Detection Threshold

Glycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods

A Concentration-Controllable Microfluidic Droplet Mixer for Mercury Ion Detection

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