Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories

Abstract

Bacillus licheniformis is a characteristic Gram-positive bacterium originally found in soil. This microorganism has long been utilised as a workhorse for production of industrial enzymes or high value-added chemicals. With ever-increasing understanding on this strain and the maturation of the genetic technique, important advances have recently been made in developing B. licheniformis as an excellent chassis cell for synthetic biology. Here, we provide an overview of updated understanding on genome information, anaerobic metabolism, industrial applications of this strain. The state-of-art B. licheniformis genetics, especially its synthetic biology advances in biosensor, expression system and artificial metabolic pathways are illustrated. Finally, perspectives are offered for the limitations and challenges to be addressed to improve B. licheniformis as microbial cell factories.

Bacillus subtilis RBT-7/32 and Bacillus licheniformis RBT-11/17 as New Promising Strains for Use in Probiotic Feed Additives

“…Along with antimicrobial properties, B. subtilis produces enzymes utilizing the products of putrefactive decay of tissues, recovers populations of microorganisms composing the intestine normoflora, and synthesizes amino acids, vitamins, and immunoactive compounds. B. licheniformis produces a number of enzymes involved into food digestion and toxin neutralization and stimulates interferon production that results in a suppression of the growth and propagation of pathogenic microorganisms and viruses facilitating the normalization of intestine microflora [28][29][30][31][32].…”

Improvement of Alpha-amylase Activity from Bacillus Licheniformis using UV Radiation and Modified Media Composition

“…These contribute a major class of industrial enzymes constituting 25% of the enzyme market (Hiteshi et al, 2016). The global market for industrial enzymes estimated at $2 billion in 2004 and expected to rise at an average annual growth rate of 3.3% (Periasamy Anbu and Lakshmipriya, 2017, He et al, 2023). Bacillus licheniformis is one of the most characterized grampositive bacteria with unique genetic background and safety characteristics.…”

“…Bacillus licheniformis is one of the most characterized grampositive bacteria with unique genetic background and safety characteristics. It has the important biologic applications in the food industry, including, the biosynthesis of high value-added bioproducts, probiotic functions, biological treatment of wastes derived from food production (He et al, 2023). B. licheniformis is a well-known amylase producer, and improvement of its amylase production is of great interest.…”

“…This species is easily differentiated from other members of the genus that are pathogenic to humans and animals. Amylases from B. licheniformis are deployed for the hydrolysis of starch, desizing of textiles and sizing of paper (He et al, 2023). In Bangladesh, α -amylase is being used in food and pharmaceuticals industries for production of high glucose and maltose syrups.…”