Recent analytical methodologies for the determination of anti-covid-19 drug therapies in various matrices: a critical review

Khalil, Hadeel A.; Hassanein, Nermeen A.; El-Yazbi, Amira F.

doi:10.1039/d3ra00654a

Cited by 4 publications

(1 citation statement)

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“…The waters C18 (150 × 4.6mm; 5 μm pore size) column at 30 °C performs best among the other columns studied in terms of resolution, symmetry and hence was selected as appropriate for the study. Literature also confirms that, this column was very efficient for resolution of various compounds and its impurities (Piponski et al 2022;Khalil et al 2023). An appropriate mobile phase was finalized by optimizing different composition of mobile phase including ammonium acetate, acetic acid, aqueous ammonia at various strengths.…”

Section: Vaka Et Alsupporting

confidence: 59%

Optimization of LC-MS/MS Analytical Method for Trace Level Quantification of Potential Genotoxic Impurities in Siponimod Pure Drug and Formulations

Reddy Vaka,

Sreenivasa Rao,

Manjula Bharathi

et al. 2024

IJALSR

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The generation of single or multiple genotoxic impurities during synthesis of siponimod should be avoided for production of safe formulation. Technically, complete elimination of genotoxic impurities was not possible and hence there is a need to propose an accurate method for trace level detection of genotoxic impurities.Method optimization studies were conducted by analysis standard solution in various method parameters. The results noticed in every varied method condition were tabulated for finalizing the appropriate conditions for analyzing siponimod. The optimized method consists of waters C18 (150 × 4.6 mm; 5 μm) column, ammonium acetate (0.02M) at pH 4.2 (fixed with 1 % formic acid) and methanol in 45:55 (v/v) at 0.5 mL/min flow rate. The mass analyser was operated in multiple reaction positive ion mode with characteristic mass transition at m/z of 517 (parent ion)and 213 (product ion) for siponimod, 434(parent ion) and 173 (product ion)for alcohol and 432(parent ion) and172 (product ion)for aldehyde impurity. No impurity or unwanted compounds detected in both LC chromatograms and mass spectra, confirming the method specificity.Validation of method for parameters including linearity, precision, recovery, ruggedness, and robustness yielded acceptable results. The method is suitable for assessing potential genotoxic impurities during the synthesis of siponimod and the manufacturing of pharmaceutical products.

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Section: Vaka Et Alsupporting

confidence: 59%

Optimization of LC-MS/MS Analytical Method for Trace Level Quantification of Potential Genotoxic Impurities in Siponimod Pure Drug and Formulations

Reddy Vaka,

Sreenivasa Rao,

Manjula Bharathi

et al. 2024

IJALSR

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First analytical confirmation of drug-induced crystal nephropathy in felines caused by GS-441524, the active metabolite of Remdesivir

Furbish,

Allinder,

Austin

et al. 2024

Journal of Pharmaceutical and Biomedical Analysis

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Rapid and ecofriendly ultra performance liquid chromatographic analytical methodology for the simultaneous determination of four drugs included in COVID-19 treatment protocol

Ghozzy,

El-Enany,

Tolba

et al. 2024

Microchemical Journal

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Recent analytical methodologies for the determination of anti-covid-19 drug therapies in various matrices: a critical review

Abstract: Since the discovery of the first case infected with severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) in Wuhan, China in December 2019, it has turned into a global pandemic.

Cited by 4 publications

References 50 publications

Optimization of LC-MS/MS Analytical Method for Trace Level Quantification of Potential Genotoxic Impurities in Siponimod Pure Drug and Formulations

Optimization of LC-MS/MS Analytical Method for Trace Level Quantification of Potential Genotoxic Impurities in Siponimod Pure Drug and Formulations

First analytical confirmation of drug-induced crystal nephropathy in felines caused by GS-441524, the active metabolite of Remdesivir

Rapid and ecofriendly ultra performance liquid chromatographic analytical methodology for the simultaneous determination of four drugs included in COVID-19 treatment protocol

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