Recent advances with recombinant bacterial vaccine vectors

Shata, Mohamed T.; Stevceva, Liljana; Agwale, Simon M; Lewis, George K.; Hone, David M.

doi:10.1016/s1357-4310(99)01633-0

Cited by 100 publications

(59 citation statements)

References 53 publications

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“…These characteristics may include (a) bacterial motility leading to uniform penetration within tumors; (b) hypoxic regions, an environment to which facultative anaerobic salmonellae are well adapted and can multiply, and in which macrophages, neutrophils, and granulocytes, effectors of bacterial clearance, are reduced in number (39); (c) both antibodies and serum complement components, which together can be lytic to salmonellae, are greatly restricted from the tumor environment by the irregular vasculature and positive pressure that exist inside tumors (40); (d) nutrients, such as high availability of glucose in aggressively growing tumors, may promote locally increased bacterial growth (41); and (e) Salmonella may induce apoptosis in infected macrophages (42) at the tumor margins leading to increased antitumor inflammatory responses. An important recent advance in this field is the development of live, attenuated Salmonella vectors for DNA vaccine delivery (43). The mechanisms involved in Salmonella delivery of DNA vaccine plasmids to the cytosol of mammalian cells is yet unclear (44).…”

Section: Cancer Researchmentioning

confidence: 99%

Intratumoral Delivery and Suppression of Prostate Tumor Growth by AttenuatedSalmonella entericaserovartyphimuriumCarrying Plasmid-Based Small Interfering RNAs

Zhang

Gao²,

Zhao³

et al. 2007

Cancer Research

132

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The facultative anaerobic, invasive Salmonella enterica serovar typhimurium (S. typhimurium) has been shown to retard the growth of established tumors. We wondered if a more effective antitumor response could be achieved in vivo if these bacteria were used as tools for delivering specific molecular antitumor therapeutics. Constitutively activated transcription factor signal transducer and activator of transcription 3 (STAT3) promotes the survival of a number of human tumors. In this study, we investigated the relative efficacies of attenuated S. typhimurium alone or combined with Stat3-specific small interfering RNA (siRNA) in terms of tumor growth and metastasis. The bacteria preferentially homed into tumors over normal liver and spleen tissues in vivo. S. typhimurium expressing plasmid-based Stat3-specific siRNAs significantly inhibited tumor growth, reduced the number of metastastic organs, and extended the life time for C57BL6 mice bearing an implanted prostate tumor, versus bacterial treatment alone. These results suggest that attenuated S. typhimurium combined with an RNA interference approach might be more effective for the treatment of primary as well as metastatic cancer. [Cancer Res 2007;67(12):5859-64]

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Section: Cancer Researchmentioning

confidence: 99%

Intratumoral Delivery and Suppression of Prostate Tumor Growth by AttenuatedSalmonella entericaserovartyphimuriumCarrying Plasmid-Based Small Interfering RNAs

Zhang

Gao²,

Zhao³

et al. 2007

Cancer Research

132

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“…Live Salmonella and Shigella vaccine vectors (39,40), cationic lipid (41), carboxymethylcellulose polymer (37), and microspheres (12-14, 33, 34) have been used to enhance mucosal DNA immunization. Alternatively, we developed reovirus protein 1-PL conjugates with the same goal in mind.…”

Section: Discussionmentioning

confidence: 99%

M cell-targeted DNA vaccination

Wang

Csencsits

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

107

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DNA immunization, although attractive, is poor for inducing mucosal immunity, thus limiting its protective value against most infectious agents. To surmount this shortcoming, we devised a method for mucosal transgene vaccination by using an M cell ligand to direct the DNA vaccine to mucosal inductive tissues and the respiratory epithelium. This ligand, reovirus protein 1, when conjugated to polylysine (PL), can bind the apical surface of M cells from nasal-associated lymphoid tissues. Intranasal immunizations with protein 1-PL-DNA complexes produced antigen-specific serum IgG and prolonged mucosal IgA, as well as enhanced cellmediated immunity, made evident by elevated pulmonary cytotoxic T lymphocyte responses. Therefore, targeted transgene vaccination represents an approach for enabling DNA vaccination of the mucosa.

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“…However, this method requires highly purified DNA, and when applied without adjuvants leads to rather weak or even adverse immune responses probably due to limited costimulatory activity. 5 Recent studies have indicated that bacteria, like Shigella flexneri, [6][7][8][9] Salmonella spp, 9,10 E. coli, 7,11 Yersinia enterocolitica 12 and Listeria monocytogenes, 9,13,14 can be used as carriers for transporting plasmids similar to those used as DNA vaccines into a variety of mammalian cells including antigen-presenting cells (APC). The term 'bactofection' has been recently coined for this bacteriamediated DNA delivery into mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery

et al. 2003

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Bacteria-mediated transfer of plasmid DNA into mammalian cells (bactofection) is a potent approach to express plasmidencoded heterologous proteins (protein antigens, toxins or enzymes) in a large set of different cell types including phagocytic and nonphagocytic mammalian cells. Previously, we have described a Listeria monocytogenes-mediated DNA delivery system, which releases plasmid DNA directly into the cytosol of mammalian cells by partial self-destruction of the carrier bacteria. Here we report on a second generation of this phage lysin supported bactofection system, which is greatly improved with respect to plasmid stability, transfer efficacy and biosafety. In this case, DNA release is initiated by spontaneous bacterial lysis in the infected cells cytosol which is subsequently enhanced by the simultaneously released phage lysin produced by the intracellular carrier bacteria. Bacteria that are capable of cell-to-cell spread are found to be much more efficient in bactofection than their nonspreading counterparts.

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Recent advances with recombinant bacterial vaccine vectors

Cited by 100 publications

References 53 publications

Intratumoral Delivery and Suppression of Prostate Tumor Growth by AttenuatedSalmonella entericaserovartyphimuriumCarrying Plasmid-Based Small Interfering RNAs

Intratumoral Delivery and Suppression of Prostate Tumor Growth by AttenuatedSalmonella entericaserovartyphimuriumCarrying Plasmid-Based Small Interfering RNAs

M cell-targeted DNA vaccination

Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery

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