Recent advances refining galactooligosaccharide production from lactose

Gosling, Aaron; Stevens, Geoffrey W.; Barber, Andrew R.; Kentish, Sandra E.; Gras, Sally L.

doi:10.1016/j.foodchem.2009.12.063

Cited by 274 publications

(243 citation statements)

References 115 publications

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“…singularis also catalyses the hydrolysis of b-glycosidic linkages, such as ONP-Glu and PNP-Glu (Blakely & Mackenzie, 1969), and possesses particularly appealing enzymic capabilities with respect to competing technologies. For instance, this unique membrane-bound enzyme possesses industrial advantages, including enzymic activity at a wide range of pH and temperatures and in the absence of cofactors or additional ions, and GOS formation independent of the initial lactose concentrations (Blakely & Mackenzie, 1969;Gosling et al, 2010;Dagher et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A novel N-terminal region of the membrane β-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

Dagher

Bruno-Bárcena

2016

Microbiology

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The b-hexosyltransferase (BHT) from Sporobolomyces singularis is a membrane-bound enzyme that catalyses transgalactosylation reactions to synthesize galacto-oligosaccharides (GOSs). To increase the secretion of the active soluble version of this protein, we examined the uncharacterized novel N-terminal region (amino acids 1-110), which included two predicted endogenous structural domains. The first domain (amino acids 1-22) may act as a classical leader while a non-classical signal was located within the remaining region (amino acids 23-110). A functional analysis of these domains was performed by evaluating the amounts of the rBHT forms secreted by recombinant P. pastoris strains carrying combinations of the predicted structural domains and the a mating factor (MFa) from Saccharomyces cerevisiae as positive control. Upon replacement of the leader domain (amino acids 1-22) by MFa (MFa-rBht (23-594) ), protein secretion increased and activity of both soluble and membrane-bound enzymes was improved 53-and 14-fold, respectively. Leader interference was demonstrated when MFa preceded the putative classical rBHT leader (amino acids 1-22), explaining the limited secretion of soluble protein by P. pastoris (GS115 : : MFa-rBht (1-594) ). To validate the role of the N-terminal domains in promoting protein secretion, we tested the domains using a non-secreted protein, the anti-b-galactosidase single-chain variable antibody fragment scFv13R4.The recombinants carrying chimeras of the N-terminal 1-110 regions of rBHT preceding scFv13R4 correlated with the secretion strength of soluble protein observed with the rBHT recombinants. Finally, soluble bioactive HIS-tagged and non-tagged rBHT (purified to homogeneity) obtained from the most efficient recombinants (GS115 : : MFa-rBht (23-594) -HIS and GS115 : : MFa-rBht ) showed comparable activity rates of GOS generation.

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Section: Introductionmentioning

confidence: 99%

A novel N-terminal region of the membrane β-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

Dagher

Bruno-Bárcena

2016

Microbiology

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“…16 Moreover, with the growing expansion of biotechnology industry, it is becoming increasingly evident to design efficient and novel procedure for obtaining such products with improved quality and at less cost. 16 It should be noted that agarose nanoparticles (ANPs) have been successfully utilized earlier for the administration of therapeutic proteins and peptides. 23,26,27 Hence, in the present study, efforts were raised to utilize these synthesized ANPs in biotechnology industries.…”

Section: Resultsmentioning

confidence: 99%

“…Hence, they are currently used as low-calorie sweeteners in fermented milk products, confectioneries, breads and beverages. 16 Moreover, with the growing expansion of biotechnology industry, it is becoming increasingly evident to design efficient and novel procedure for obtaining such products with improved quality and at less cost. 17 It should be noted that major drawback associated in using free enzymes for biotechnological applications involves their instability at different pH and temperature environments.…”

Section: Introductionmentioning

confidence: 99%

Utility of Functionalized Agarose Nanoparticles in Hydrolyzing Lactose in Batch Reactors for Dairy Industries

Ansari¹,

Ahmad²,

Jafri³

et al. 2018

Quim. Nova

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The present study investigates the synthesis of agarose nanoparticles (ANPs) and its surface modification by galactose for the immobilization of β-galactosidase. Galactose modified ANPs retained 91% enzyme activity upon immobilization. Optimum pH (4.5) and temperature (50 °C) remains unchanged after immobilization. However, immobilized enzyme retained greater catalytic activity against lower and higher, pH and temperature ranges. Immobilized β-galactosidase retained 89% biocatalytic activity even at 4% galactose concentration as compared to enzyme in solution, and exhibited 81% activity even after seventh repeated uses. Immobilized enzyme hydrolyzed greater amount of lactose at higher temperatures as compared to β-galactosidase in solution, thereby suggesting its potential application in obtaining lactose-free dairy products at large scale.

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“…Nanofiltration membranes can be used for purifying galactooligosaccharides (GOS) products from the monosaccharide hydrolysis products, glucose and galactose. According to Gosling et al, (2010), up to 88% of the di-and oligosaccharides were recovered from a commercial GOS mixture using a nanofiltration membrane with only 19% of the monosaccharides remaining in the retentate stream (Goulas et al, 2003). Catarino et al, (2008) investigated the fractionation of saccharide mixtures with calcium using ultrafiltration and nanofiltration processes and reported that saccharide fractionation was enhanced in the presence of calcium.…”

Section: Oligosaccharidesmentioning

confidence: 99%

Continuous Membrane Bioreactor (CMBR) to Produce Nanoparticles from Milk Components

Nieto-Nieto¹,

Amaya-Llano²,

Ozimek³

2012

Trends in Vital Food and Control Engineering

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Recent advances refining galactooligosaccharide production from lactose

Cited by 274 publications

References 115 publications

A novel N-terminal region of the membrane β-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

A novel N-terminal region of the membrane β-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

Utility of Functionalized Agarose Nanoparticles in Hydrolyzing Lactose in Batch Reactors for Dairy Industries

Continuous Membrane Bioreactor (CMBR) to Produce Nanoparticles from Milk Components

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