Recent advances on protein separation and purification methods

Liu, Shixiang; Li, Zhihua; Yu, Bing; Wang, Song; Shen, Youqing; Cong, Hailin

doi:10.1016/j.cis.2020.102254

Cited by 138 publications

(74 citation statements)

References 182 publications

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“…Membrane separation is beneficial compared to other separation processes due to high selectivity and separation efficiency, no need for the use of additives or chemicals, reducing operating costs as well as minimizing the human health risks [ 3 ]. Conventional separation techniques, such as precipitation, crystallization, and centrifugation are known to suffer from poor selectivity of separation, whereas high-resolution techniques, such as chromatography and electrophoresis, are characterized by low yield of product at higher cost [ 4 ]. Ultrafiltration is a pressure-driven membrane process which requires the use of porous membranes.…”

Section: Introductionmentioning

confidence: 99%

“…Ultrafiltration is a pressure-driven membrane process which requires the use of porous membranes. Ultrafiltration can be used for water treatment, purification, fractionation, and concentration of the solutions of polymers in microbiology, biotechnology, dairy, food industries, cosmetics, and pharmaceuticals [ 3 , 4 , 5 ]. Ultrafiltration is known to demonstrate very high process performance and can be fine-tuned to provide high selectivity [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration

2021

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This study deals with the modification of polyphenylsulfone ultrafiltration membranes by introduction of an incompatible polymer polysulfone to the polyphenylsulfone casting solution to improve the permeability. The correlation between properties of the blend polyphenylsulfone/polysulfone solutions and porous anisotropic membranes for ultrafiltration prepared from these solutions was revealed. The blend polyphenylsulfone/polysulfone solutions were investigated using a turbidity spectrum method, optical microscopy and measurements of dynamic viscosity and turbidity. The structure of the prepared blend flat sheet membranes was studied using scanning electron microscopy. Membrane separation performance was investigated in the process of ultrafiltration of human serum albumin buffered solutions. It was found that with the introduction of polysulfone to the polyphenylsulfone casting solution in N-methyl-2-pyrrolidone the size of supramolecular particles significantly increases with the maximum at (40–60):(60:40) polyphenylsulfone:polysulfone blend ratio from 76 nm to 196–354 nm. It was shown that polyphenylsulfone/polysulfone blend solutions, unlike the solutions of pristine polymers, are two-phase systems (emulsions) with the maximum droplet size and highest degree of polydispersity at polyphenylsulfone/polysulfone blend ratios (30–60):(70–40). Pure water flux of the blend membranes passes through a maximum in the region of the most heterogeneous structure of the casting solution, which is associated with the imposition of a polymer-polymer phase separation on the non-solvent induced phase separation upon membrane preparation. The application of polyphenylsulfone/polysulfone blends as membrane-forming polymers and polyethylene glycol (Mn = 400 g·mol−1) as a pore-forming agent to the casting solutions yields the formation of ultrafiltration membranes with high membrane pure water flux (270 L·m−2·h−1 at 0.1MPa) and human serum albumin rejection of 85%.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration

2021

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“…Their fundamental role in the extracellular matrix [1] and their extended applications in biotechnology, pharmaceutics [2] and food science [3,4] are inextricably linked with their molecular structure. Solubilization of hydrophobic compounds [5], binding of proteins [6] and cell internalization [7] are subjects of undiminished interest where synthetic polyelectrolytes unambiguously play a key role. Therefore, the field of polyelectrolytes is always in the center of scientific interest, not only in relation to fundamental understanding of their interactions and nanostructure, but also in applied research.…”

Section: Introductionmentioning

confidence: 99%

Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

Papagiannopoulos¹

2021

Macromol

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Polyelectrolytes have been at the center of interdisciplinary research for many decades. In the field of polymer science and soft matter, they have provided the dimensions of electrostatic interactions, which opens a vast variety of opportunities for new physical properties and applications. In biological matter, polyelectrolytes are present in many forms, from extracellular polysaccharides to complex DNA molecules and proteins. This review discusses the recent research on polyelectrolytes covering the fundamental level of their conformations and nanostructures, their molecular interactions with materials that have close relevance to bioapplications and their applications in the biomedical field. This approach is motivated by the fact that the polyelectrolyte research is constantly active in all the aforementioned levels and continually affects many critical scientific areas.

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“…Among the methods used for protein separation is the High-performance Liquid Chromatography (HPLC), which in addition to versatility has a high analytical capacity. The technique uses a liquid mobile phase responsible for separating the sample components that will flow through a column and providing data on the separation of the compounds (Coskun, 2016;Liu et al, 2020). Often the results obtained during the analyzes are complex and require multivariate techniques to assist in the interpretation of the data.…”

Section: Introductionmentioning

confidence: 99%

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

et al. 2021

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The search for origin plant-based proteases increases gradually due to their diversity and stability over a wide range of pH and temperature. Artichoke (Cynara scolymus) flowers are a proteolytic vegetable source already studied, but their leaves are scarce in this respect. Thus, the objective of this research was to obtain extracts of artichoke leaves with different buffers and extraction methods as an alternative proteolytic source and plant coagulant, as well as the separation and comparison of the protein profile of these extracts. The methodology used was based on extraction with sodium citrate buffer (pH 3), sodium acetate (pH 5) and Tris-HCl (pH 7) by mechanical stirrer (MS) and ultrasound (US); protein determination; proteolytic activity (PA) and specific activity (SA); milk clotting activity (MCA) and rennet substitution potential (RSP); high- performance liquid chromatography analysis (HPLC) with UV-Vis detector and principal component analysis (PCA). Extracts of Cynara scolymus leaves showed high results with Citrate-US for the parameters PA (14.38), SA (19.71), MCA (440) and RSP (30.60) compared to other treatments. The extracts with citrate and acetate presented a quick coagulation time (max 3 min). The HPLC analysis enabled the separation of the different protein compounds present in the extracts and most expressive peaks in the samples with Citrate-MS and Acetate-MS; and isolated peaks for Citrate-US. It was concluded that extracts of artichoke leaves with citrate and acetate buffer attributed satisfactory results to act as plant coagulant, as well as to carry out further studies for the purification of proteolytic enzymes and application in cheeses.

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Recent advances on protein separation and purification methods

Cited by 138 publications

References 182 publications

Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration

Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration

Current Research on Polyelectrolyte Nanostructures: From Molecular Interactions to Biomedical Applications

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

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