Recent advances on paper-based microfluidic devices for bioanalysis

Silva-Neto, Habdias A.; Arantes, Iana V. S.; Ferreira, André; Nascimento, Guida H.M. do; Meloni, Gabriel N.; Araújo, Wanderley Pereira de; Paixão, Thiago R.L.C.; Coltro, Wendell K. T.

doi:10.1016/j.trac.2022.116893

Cited by 29 publications

(9 citation statements)

References 154 publications

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“…SEM images involving cross-sectional views are exhibited in Figures A and Figure S3 and the thickness values are estimated as 314, 226, and 206 μm for conductive ink deposited on vegetal paper, chromatographic paper, and polystyrene film, respectively. While the polystyrene used as substrate exhibited poor adhesion with the conductive ink, the low thickness of carbon ink observed on chromatographic paper can be justified by the large porosity of the cellulose-based fibers (1 to 10 μm) in which the target ink disperses. On the other hand, the better thickness values of carbon ink measured on vegetal paper as insulating substrate can be due to a suitable interconnection between ABS and paraffin structures incorporated under cellulose fibers, and the fact that waterproof paper possesses low porosity, as demonstrated in recent studies. − …”

Section: Resultsmentioning

confidence: 99%

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Silva-Neto

Duarte-Junior

Rocha

et al. 2023

ACS Appl. Mater. Interfaces

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Here, we propose a recyclable approach using acrylonitrile-butadiene-styrene (ABS) residues from additive manufacturing in combination with low-cost and accessible graphite flakes as a novel and potential mixture for creating a conductive paste. The graphite particles were successfully incorporated in the recycled thermoplastic composite when solubilized with acetone and the mixture demonstrated greater adherence to different substrates, among which cellulose-based material made possible the construction of a paper-based electrochemical sensor (PES). The morphological, structural, and electrochemical characterizations of the recycled electrode material were demonstrated to be similar to those of the traditional carbon-based surfaces. Faradaic responses based on redox probe activity ([Fe(CN)6]3‑/4–) exhibited well-defined peak currents and diffusional mass transfer as a quasi-reversible system (96 ± 5 mV) with a fast heterogeneous rate constant value of 2 × 10–3 cm s–1. To improve the electrode electrochemical properties, both the PES and the classical 3D-printed electrode surfaces were modified with a combination of multiwalled carbon nanotubes (MWCNTs), graphene oxide (GO), and copper. Both electrode surfaces demonstrated the suitable oxidation of nitrite at 0.6 and 0.5 V vs Ag, respectively. The calculated analytical sensitivities for PES and 3D-printed electrodes were 0.005 and 0.002 μA/(μmol L–1), respectively. The proposed PES was applied for the indirect amperometric analysis of S-nitroso-cysteine (CysNO) in serum samples via nitrite quantitation, demonstrating a limit of detection of 4.1 μmol L–1, with statistically similar values when compared to quantitative analysis of the same samples by spectrophotometry (paired t test, 95% confidence limit). The evaluated electroanalytical approach exhibited linear behavior for nitrite in the concentration range between 10 and 125 μmol L–1, which is suitable for realizing clinical diagnosis involving Parkinson’s disease, for example. This proof of concept shows the great promise of this recyclable strategy combining ABS residues and conductive particles in the context of green chemical protocols for constructing disposable sensors.

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Section: Resultsmentioning

confidence: 99%

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Silva-Neto

Duarte-Junior

Rocha

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

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“…Outstanding efforts have been devoted to µPADs, primarily motivated by the promise of achieving the best trade‐off between performance, affordability, and simplicity (Ahmed et al, 2016). Some shortcomings must be addressed, such as resolution, fidelity of microstructures, development of substates entirely dedicated to that field, increased accessibility of modeling software, and chemical stability of sensing materials (Silva‐Neto et al, 2023). In a real and complex sample, a detection strategy with high sensitivity and selectivity is still required (Ge et al, 2014).…”

Section: Market Demand and Future Prospectsmentioning

confidence: 99%

Evaluation of food μPADs with the new tech perspectives and future prospects

Okutan Arslan,

Trabzon

2023

eFood

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Food microfluidic paper‐based analytical devices (µPADs) are powerful tools to create total analysis systems and have long been demonstrated to be useful for safety and quality applications. Thanks to new technological innovations food µPADs offer exciting new possibilities. This review introduces how µPADs are obtained from chromatography, filter, or office papers and detect analytes from a food sample. We introduce the most current developments in the use of µPADs with an emphasis on paper types, adapted new technologies, and detection limits. Classifications are also made on food µPADs according to applied innovations and adapted novel technologies. In the first section, simple forms of µPADs for the detection of pathogen, mycotoxin, pesticides, and other food components are discussed with technical details. Then, we introduce multisensing approaches for high throughput analysis and a concise summary will be given. In the case of three‐dimensional (3D) µPADs, the use of 3D is discussed and compared to 2D µPADs in terms of its advantageous with the example of a food colorant test device. In the following section, smartphone adoption to µPADs is introduced in detail with eight different assay examples. Centrifugal platform for nickel assay is demonstrated as an enabling approach with shortened assay times by rotational velocity. The potential of user‐friendly hybrid devices is also summarized in the last part. Finally, we present an outlook to underline the opportunities created by smartphone‐based and intelligent µPADs for food safety and quality with real success perspectives

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“…[3][4][5][6] Compared to traditional serological immunoassays, non-invasive urine retrieval without complex sampling procedures and expensive automated testing instruments has become a new trend in determining the potential prevalence of prostate cancer in individuals. [7,8] Sarcosine, a glycine N-methyl derivative, has been identified as a highly elevated metabolite in the metastatic process of prostate cancer and can be determined through non-invasive urine sample collection and recovery. [9][10][11][12] It has been validated that sarcosine, as a test indicator for early PC, has a detectable concentration of 1-3 µm in normal urine, while it usually shows an increase of more than one order of magnitude in pathological processes.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐Encapsulated Protein Trap Engineered Metal–Organic Framework‐Derived Biomineral Probes for Non‐Invasive Prostate Cancer Surveillance

Tang

Gong

et al. 2023

Adv Funct Materials

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A paper‐based naked‐eye recognition assay with enzyme‐encapsulated protein engineered metal–organic framework‐derived biominerals is developed for direct quantification of sarcosine in urine samples for screening of prostate cancer individuals. The detection strategy stems from the successful construction of a cascade response model, which involves the introduction of a cascade enzymatic catalytic reaction on Pt nanoparticles (NPs)‐loaded porous CeO2 by integrating a sarcosine oxidase as a special recognition unit and a chromogenic substrate as a signal molecule reporter. Pt NPs‐loaded CeO2 is subjected to a one‐step thermal treatment based on multilayered mesoporous Ce‐based metal–organic framework, and the calcined CeO2 exhibits the same distinct porous graded structure. Importantly, introduction of Pt NPs sharply enhances the peroxidase‐like activity of CeO2, which is considered to be caused by the difference in the adsorption behavior of hydrogen peroxide on the CeO2 surface and Pt/CeO2 obtained by density functional theory calculations. On the basis of this, the probe is used on a mass‐producible paper‐based working platform and 3D‐printed device to specifically screen for minor differences in sarcosine between urine samples from cancer patients and normal individuals. Enzyme‐assisted cascade catalytic reaction can be extended by replacing different recognition units for multiple analytes.

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Recent advances on paper-based microfluidic devices for bioanalysis

Cited by 29 publications

References 154 publications

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Evaluation of food μPADs with the new tech perspectives and future prospects

Enzyme‐Encapsulated Protein Trap Engineered Metal–Organic Framework‐Derived Biomineral Probes for Non‐Invasive Prostate Cancer Surveillance

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