Recent Advances on Acellular Cementum Increments Composition Using Synchrotron X-Radiation

Naji, Stephan; Stock, Stuart R.; Rendu, William; Gourichon, Lionel; Colard, Thomas; Cai, Zhonghou

doi:10.1017/9781108569507.008

Cited by 7 publications

(15 citation statements)

References 15 publications

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“…Elevated Zn concentrations are often associated with ongoing biomineralization ( Naji et al, 2022 ) and have been demonstrated to be preserved in structures such as cementum and dentin growth bands ( Stock et al, 2017 ; Ryan et al, 2020 ). A rather faint (background) Zn staining of lamellar structures of primary osteons that resembles our findings in the roe deer antlers ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Distribution, structure, and mineralization of calcified cartilage remnants in hard antlers

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Distribution, structure, and mineralization of calcified cartilage remnants in hard antlers

et al. 2022

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“…In a previous study on a human tooth with erythroblastosis fetalis, the reported beam size during WAXS analysis was 0.53 × 0.39 µm 2 [ 2 ]; however, a step of 2.6 µm, higher than previous studies was used [ 414 ]. A higher resolution was used to study archaeological samples of human cementum and dentine at 250 nm [ 310 ], and 200 nm and 120 nm for bone [ 577 , 578 ]. To date, the use of high-resolution diffraction analysis in dental research has not been widely applied.…”

Section: Synchrotron (Circular Particle Accelerator)mentioning

confidence: 99%

“…In addition to the improvement in spatial resolution, the combination of diffraction with other techniques that enable correlative analysis, such as spectroscopy, has been reported for cementum (using a resolution of 250 nm) [ 310 ], as well as the incorporation of tomography for bone [ 577 , 578 , 579 ]. In a study on caries, multi-dimensional WAXS analysis correlated with tomography [ 448 ] (at a resolution of 150 µm) revealed details of the lattice parameter and nanocrystallite size and suggested amorphisation of the crystal structure ( Figure 6 a).…”

Section: Synchrotron (Circular Particle Accelerator)mentioning

confidence: 99%

“…One of the analyses provided 2D maps of the distribution of the investigated elements with a high resolution, down to 1 µm (1 × 0.5 µm 2 of beam size) [ 169 ], Figure 9 c. More recently, the resolution was lowered further in the study of a human tooth from a patient suffering from HELIX syndrome, where a beam size of 500 × 500 nm 2 was used to show the variation of Sr in enamel and dentine, which was suggested to be from a renal dysfunction [ 308 ]. In another study, a resolution of 250 nm was used to analyse the variation of Ca and Zn intensity in human cementum and dentine [ 310 ]. A higher resolution than these studies was also used to characterise carious enamel and the preferential demineralisation occurring in a sample.…”

Section: Synchrotron (Circular Particle Accelerator)mentioning

confidence: 99%

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Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

et al. 2023

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Hard dental tissues possess a complex hierarchical structure that is particularly evident in enamel, the most mineralised substance in the human body. Its complex and interlinked organisation at the Ångstrom (crystal lattice), nano-, micro-, and macro-scales is the result of evolutionary optimisation for mechanical and functional performance: hardness and stiffness, fracture toughness, thermal, and chemical resistance. Understanding the physical–chemical–structural relationships at each scale requires the application of appropriately sensitive and resolving probes. Synchrotron X-ray techniques offer the possibility to progress significantly beyond the capabilities of conventional laboratory instruments, i.e., X-ray diffractometers, and electron and atomic force microscopes. The last few decades have witnessed the accumulation of results obtained from X-ray scattering (diffraction), spectroscopy (including polarisation analysis), and imaging (including ptychography and tomography). The current article presents a multi-disciplinary review of nearly 40 years of discoveries and advancements, primarily pertaining to the study of enamel and its demineralisation (caries), but also linked to the investigations of other mineralised tissues such as dentine, bone, etc. The modelling approaches informed by these observations are also overviewed. The strategic aim of the present review was to identify and evaluate prospective avenues for analysing dental tissues and developing treatments and prophylaxis for improved dental health.

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“…The tooth cementum, i.e. the interface between the alveolar bone and the root dentin, contains unique growth features as recognized in optical micrographs of tooth sections in the 1950s [2]. The preparation of these slices is based physical sectioning, a procedure to be avoided for valuable unique objects including archeological teeth.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional imaging and analysis of annual layers in tree trunk and tooth cementum

Müller¹,

Stiefel

Rodgers³

et al. 2022

Bioinspiration, Biomimetics, and Bioreplication XII

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The growth of plants, animals, and humans can give rise to layered structures associated with annual periodicity. Thickness variations are often correlated to nutrition supply and stress factors. The annual layers in a tree trunk with millimeter thickness can be directly counted, whereas the layers in tooth cementum with micrometer thickness are made visible using optical microscopy. These optical techniques rely on the surface evaluation or thin, optically transparent slices. Hard Xray tomography with micrometer resolution, however, provides a three-dimensional view without physical slicing. We have developed a procedure to enhance the tomography data of annual layers in human and bovid tooth cementum. The analysis of a substantial part of an archeological human tooth demonstrated that the detected number of layers depended on the selected region and could vary between 13 and 27. The related average thickness of the annual layers was found to be (5.4 ± 1.9) µm for the human tooth, whereas the buffalo tooth exhibited a layer periodicity of 46 µm. The present study elucidates the potential of combining computational tools with high-quality micro computed tomography data to quantify the annual layers in tooth cementum for a variety of purposes including age-at-death determination.

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Recent Advances on Acellular Cementum Increments Composition Using Synchrotron X-Radiation

Cited by 7 publications

References 15 publications

Distribution, structure, and mineralization of calcified cartilage remnants in hard antlers

Distribution, structure, and mineralization of calcified cartilage remnants in hard antlers

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

Three-dimensional imaging and analysis of annual layers in tree trunk and tooth cementum

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