Recent advances of single molecule/particle imaging with optical microscopic methods

Abstract

Optical mapping of a rice BAC clone using restriction endonuclease and imaging with fluorescent microscopy at single molecule level Chinese Science Bulletin 47, 319 (2002); Special topic for "single-molecule, single-particle and single-cell bioimaging"

Single-Particle Tracking with Scattering-Based Optical Microscopy

“…Traditional methods are commonly focusing on ensemble measurements and obtain averaged properties from large amounts of objects, while the heterogeneous behaviors of individual dynamics like molecular–molecular interactions are commonly ignored. With the development of optical microscopic techniques, single-particle tracking (SPT) as a robust technique to directly differentiate the precise characteristics and dynamics of individual objects, has been extensively studied in recent years. − The distribution of heterogeneous behaviors can be characterized by SPT, providing a better understanding on the detailed kinetic mechanism of many biological processes.…”