Recent advances of integrated microfluidic suspension cell culture system

Abstract

Microfluidic devices with superior microscale fluid manipulation ability and large integration flexibility offer great advantages of high throughput, parallelisation and multifunctional automation. Such features have been extensively utilised to facilitate cell culture processes such as cell capturing and culturing under controllable and monitored conditions for cell‐based assays. Incorporating functional components and microfabricated configurations offered different levels of fluid control and cell manipulation strategies to meet diverse culture demands. This review will discuss the advances of single‐phase flow and droplet‐based integrated microfluidic suspension cell culture systems and their applications for accelerated bioprocess development, high‐throughput cell selection, drug screening and scientific research to insight cell biology. Challenges and future prospects for this dynamically developing field are also highlighted.

Role of Cytokines as Biomarkers for Disease Severity in Bacterial and Viral Infections.

“…2. Microfluidics-Based Platforms: By virtue of microfluidics, the cytokine assays that involve a high level of automation and sample-saving can be realized, which leads in turn to the higher sensitivity and in vitro high-throughput screening in a compact format (49) . 3.…”

Culturing cells for life: innovative approaches in macroscopic and microfluidic cultures, with an emphasis on stem cells

“…Over the past decades, the potential of microfluidics to change cell biology research had become gradually a reality (Scheme 3). The similitude of the dimensions of microfluidic channels and the size of cells, and the ability to manipulate small volumes of fluid in micrometre-scale channels, together with many other important advantages of microfluidics such as flexibility, rapid energy dissipation, and a fast-prototyping fabrication for customized experimental design, make microfluidics suitable for cell culture and for cells' subsequent analysis (Tehranirokh et al, 2013;Mehling and Tay, 2014;Kerk et al, 2021). Microfluidics reduced cell populations to a few hundred cells, compared to macroscopic cell cultures that contain around 10 4 -10 7 cells (Halldorsson et al, 2015).…”

“…An electrical potential is applied to an array of insulated electrodes to actuate the droplets Cells that grow while suspended in a liquid culture medium may be further manipulated by the use of electrical, optical, or magnetic forces. To enhance suspension culture performance, mamallian and microbial cells are encapsulated in hydrogel beads as a scaffold to create a 3D environment (Mulas et al, 2020;Kerk et al, 2021).…”