Recent Advances of DNA Hydrogels in Biomedical Applications

Chen, Huai-Qing; Yang, Wan; Zuo, Hua; He, Huawei; Wang, Yejing

doi:10.1007/s41664-021-00185-w

Cited by 9 publications

(3 citation statements)

References 100 publications

(107 reference statements)

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“…[22] Based on this property and many characteristics of DNA, stimuli-responsive selfdegradable DNA hydrogels are widely used in bioanalysis and biomedicine, playing an increasingly important role as intelligent platforms. [23] Here, we summarize the design, synthesis, and applications of stimuli-responsive self-degradable DNA hydrogel, focusing on the stimulus-responsive nucleic acid structures and how various trigger stimuli contribute to hydrogel self-degradation (Scheme 1). In addition, we discuss the stimuli-responsive self-degradable DNA hydrogels under different stimulation conditions and their applications in biosensing, drug delivery, and cell capture and release.…”

Section: Introductionmentioning

confidence: 99%

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Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Wang

Duan

Liu

et al. 2023

Adv Healthcare Materials

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DNA hydrogels play an increasingly important role in biomedicine and bioanalysis applications. Due to their high programmability, multifunctionality and biocompatibility, they are often used as effective carriers for packing drugs, cells, or other bioactive cargoes in vitro and in vivo. However, the stability of the DNA hydrogels prevents their in‐demand rapid release of cargoes to achieve a full therapeutic effect in time. For bioanalysis, the generation of signals sometimes needs the DNA hydrogel to be rapidly degraded when sensing target molecules. To meet these requirements, stimulus‐responsive DNA hydrogels are designed. By responding to different stimuli, self‐degradable DNA hydrogels can switch from gel to solution for quantitative bioanalysis and precision cargo delivery. This review summarizes the recently developed innovative methods for designing stimuli‐responsive self‐degradable DNA hydrogels and showed their applications in the bioanalysis and biomedicines fields. Challenges, as well as prospects, are also discussed.

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Section: Introductionmentioning

confidence: 99%

“…[ 22 ] Based on this property and many characteristics of DNA, stimuli‐responsive self‐degradable DNA hydrogels are widely used in bioanalysis and biomedicine, playing an increasingly important role as intelligent platforms. [ 23 ]…”

Section: Introductionmentioning

confidence: 99%

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Wang

Duan

Liu

et al. 2023

Adv Healthcare Materials

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“…19 Especially, pure DNA hydrogels, composed of pure DNAs, have attracted extensive attention. 20 The construction of pure DNA hydrogels is generally through the self-assembly of DNA fragments or the enzymatic amplification of extremely long DNA chains, 21 thus exhibiting the superiority of facile synthesis over DNA hybrid hydrogels. 22 For instance, Huang et al have demonstrated that a rolling circle amplification (RCA) reaction could generate a macroscale pure DNA hydrogel in a programmable manner for sensitive and accurate assay of glucose.…”

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confidence: 99%

Smartphone-Based Pure DNAzyme Hydrogel Platform for Visible and Portable Colorimetric Detection of Cell-Free DNA

et al. 2022

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Cell-free DNA (cfDNA), as a tumor marker, is of great importance for the diagnosis of cancer and targeted therapy. However, the need for huge analytical instruments for cfDNA analysis has restricted its practical applications, especially in rural areas and third-world countries. Herein, a portable and visual smartphone-based DNAzyme hydrogel platform is developed for cfDNA detection. The target cfDNA triggers rolling circle amplification to produce a G-quadruplex-comprised DNA hydrogel with an horseradish peroxidase (HRP)-like catalytic function, which further catalyzes the chromogenic substrate to generate a visible output signal. Notably, the naked-eye detection of cfDNA can be realized by the macroscale visibility and catalytic ability of the DNA hydrogel. The linear range of the DNAzyme hydrogel platform for cfDNA detection is 0.1 pM−1500 nM with a detection limit of 0.042 pM. Moreover, this platform is exploited for the detection of cfDNA in spiked human serum with favorable sensitivity and recovery. Therefore, the DNAzyme hydrogel platform provides highly promising potential for testing other nucleic acid biomarkers.

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Biodegradable and Antioxidant DNA Hydrogel as a Cytokine Delivery System for Diabetic Wound Healing

Wang

Gou

et al. 2022

Adv Healthcare Materials

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Impaired diabetic wound healing is associated with the persistence of chronic inflammation and excessive oxidative stress, which has become one of the most serious clinical challenges. Wound dressings with anti-inflammatory and reactive oxygen species (ROS)-scavenging properties are desirable for diabetic wound treatment. In this study, a shape-adaptable, biodegradable, biocompatible, antioxidant, and immunomodulatory interleukin-33 (IL-33)-cytogel is developed by encapsulating IL-33 into physically cross-linked DNA hydrogels and used as wound dressings to promote diabetic wound healing. The porous microstructures and biodegradable properties of the IL-33-cytogel ensure the local sustained-release of IL-33 in the wound area, where the sustained-release of IL-33 is maintained for at least 7 days. IL-33-cytogel can induce local accumulation of group 2 innate lymphoid cells (ILC2s) and regulatory T cells (Tregs), as well as M1-to-M2 transition at the wound sites. Additionally, the antioxidant and biocompatible characteristics of DNA hydrogels promote the scavenging of intracellular ROS without affecting cell viability. As a result, local inflammation in the diabetic wound area is resolved upon IL-33-cytogel treatment, which is accompanied by improved granulation tissue regeneration and accelerated wound closure. This study demonstrates a promising strategy in tissue engineering and regenerative medicine by incorporating DNA hydrogels and cytokine immunotherapy for promoting diabetic wound healing.

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Recent Advances of DNA Hydrogels in Biomedical Applications

Cited by 9 publications

References 100 publications

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Smartphone-Based Pure DNAzyme Hydrogel Platform for Visible and Portable Colorimetric Detection of Cell-Free DNA

Biodegradable and Antioxidant DNA Hydrogel as a Cytokine Delivery System for Diabetic Wound Healing

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