Recent advances in understanding the extracellular calcium-sensing receptor

Colella, Matilde; Gerbino, Andrea; Hofer, Aldebaran M.; Curci, Silvana

doi:10.12688/f1000research.8963.1

Cited by 20 publications

(5 citation statements)

References 151 publications

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“…Thus, it will be possible to finely tune T. officinale effects on Ca 2+ -signaling in order to improve the biological outcome. Second, Ca 2+ signaling intersects the activity of a huge number of other signaling molecules (e.g., cAMP [ 45 ]) and/or effectors (e.g., mitogen-activated protein (MAP) kinases [ 46 ]). Thus, it is likely that T. officinale -induced Ca 2+ signaling activates other pathways, which have to be brought to light by tissue-specific research projects.…”

Section: Discussionmentioning

confidence: 99%

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Gerbino

Russo

Colella

et al. 2018

IJMS

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Dandelion (Taraxacum officinale Weber ex F.H.Wigg.) has been used for centuries as an ethnomedical remedy. Nonetheless, the extensive use of different kinds of dandelion extracts and preparations is based on empirical findings. Some of the tissue-specific effects reported for diverse dandelion extracts may result from their action on intracellular signaling cascades. Therefore, the aim of this study was to evaluate the effects of an ethanolic dandelion root extract (DRE) on Ca2+ signaling in human embryonic kidney (HEK) 293 cells. The cytotoxicity of increasing doses of crude DRE was determined by the Calcein viability assay. Fura-2 and the fluorescence resonance energy transfer (FRET)-based probe ERD1 were used to measure cytoplasmic and intraluminal endoplasmic reticulum (ER) Ca2+ levels, respectively. Furthermore, a green fluorescent protein (GFP)-based probe was used to monitor phospholipase C (PLC) activation (pleckstrin homology [PH]–PLCδ–GFP). DRE (10–400 µg/mL) exposure, in the presence of external Ca2+, dose-dependently increased intracellular Ca2+ levels. The DRE-induced Ca2+ increase was significantly reduced in the absence of extracellular Ca2+. In addition, DRE caused a significant Ca2+ release from the ER of intact cells and a concomitant translocation of PH–PLCδ–GFP. In conclusion, DRE directly activates both the release of Ca2+ from internal stores and a significant Ca2+ influx at the plasma membrane. The resulting high Ca2+ levels within the cell seem to directly stimulate PLC activity.

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Section: Discussionmentioning

confidence: 99%

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Gerbino

Russo

Colella

et al. 2018

IJMS

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“…CaSR is the most studied G-protein coupled receptor that senses increases of extracellular calcium [314]. This receptor is expressed by many different cell lineages and is involved in myriad of functions [315]. In the skin, calcium is crucial for the differentiation of keratinocytes in the healthy epidermis, as a co-factor in the coagulation cascade, and is implicated in the healing process [307].…”

Section: Inorganic Signallingmentioning

confidence: 99%

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Castaño

Pérez‐Amodio

Nieva

et al. 2018

Advanced Drug Delivery Reviews

149

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Skin wound healing aims to repair and restore tissue through a multistage process that involves different cells and signalling molecules that regulate the cellular response and the dynamic remodelling of the extracellular matrix. Nowadays, several therapies that combine biomolecule signals (growth factors and cytokines) and cells are being proposed. However, a lack of reliable evidence of their efficacy, together with associated issues such as high costs, a lack of standardization, no scalable processes, and storage and regulatory issues, are hampering their application. In situ tissue regeneration appears to be a feasible strategy that uses the body's own capacity for regeneration by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the wound site to promote repair and regeneration. The aim is to engineer instructive systems to regulate the spatio-temporal delivery of proper signalling based on the biological mechanisms of the different events that occur in the host microenvironment. This review describes the current state of the different signal cues used in wound healing and skin regeneration, and their combination with biomaterial supports to create instructive microenvironments for wound healing.

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“… Schematic illustration of intracellular transduction cascades activated by orthosteric agonists or allosteric modulators on the CaR. Modified from [ 110 ]. …”

Section: Figurementioning

confidence: 99%

The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology

Gerbino

Colella

2018

IJMS

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The current interest of the scientific community for research in the field of calcium sensing in general and on the calcium-sensing Receptor (CaR) in particular is demonstrated by the still increasing number of papers published on this topic. The extracellular calcium-sensing receptor is the best-known G-protein-coupled receptor (GPCR) able to sense external Ca2+ changes. Widely recognized as a fundamental player in systemic Ca2+ homeostasis, the CaR is ubiquitously expressed in the human body where it activates multiple signalling pathways. In this review, old and new notions regarding the mechanisms by which extracellular Ca2+ microdomains are created and the tools available to measure them are analyzed. After a survey of the main signalling pathways triggered by the CaR, a special attention is reserved for the emerging concepts regarding CaR function in the heart, CaR trafficking and pharmacology. Finally, an overview on other Ca2+ sensors is provided.

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Recent advances in understanding the extracellular calcium-sensing receptor

Cited by 20 publications

References 151 publications

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology

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