Recent advances in understanding of the DNA double-strand break repair machinery of plants

Bleuyard, Jean-Yves; Gallego, María; White, Charles I.

doi:10.1016/j.dnarep.2005.08.017

Cited by 94 publications

(120 citation statements)

References 143 publications

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“…In plants, RAD51 (Doutriaux et al, 1998;Bleuyard et al, 2006) and RAD54 (Osakabe et al, 2006;Shaked et al, 2006) homologs have been identified, leaving the question of whether plants contain RAD52 homologs (Iyer et al, 2002;Mortensen et al, 2009) or whether their absence may be compensated for by two plant BRCA2 homologs (Siaud et al, 2004;Abe et al, 2009). Nevertheless, the absence of RAD52 in plants is somewhat intriguing, considering its role in mediating critical steps of homologous recombination and its relatively high conservation across eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Identification of PlantRAD52Homologs and Characterization of theArabidopsis thaliana RAD52-Like Genes

et al. 2011

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RADiation sensitive52 (RAD52) mediates RAD51 loading onto single-stranded DNA ends, thereby initiating homologous recombination and catalyzing DNA annealing. RAD52 is highly conserved among eukaryotes, including animals and fungi. This article reports that RAD52 homologs are present in all plants whose genomes have undergone extensive sequencing. Computational analyses suggest a very early RAD52 gene duplication, followed by later lineage-specific duplications, during the evolution of higher plants. Plant RAD52 proteins have high sequence similarity to the oligomerization and DNA binding N-terminal domain of RAD52 proteins. Remarkably, the two identified Arabidopsis thaliana RAD52 genes encode four open reading frames (ORFs) through differential splicing, each of which specifically localized to the nucleus, mitochondria, or chloroplast. The A. thaliana RAD52-1A ORF provided partial complementation to the yeast rad52 mutant. A. thaliana mutants and RNA interference lines defective in the expression of RAD52-1 or RAD52-2 showed reduced fertility, sensitivity to mitomycin C, and decreased levels of intrachromosomal recombination compared with the wild type. In summary, computational and experimental analyses provide clear evidence for the presence of functional RAD52 DNA-repair homologs in plants.

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Section: Introductionmentioning

confidence: 99%

Identification of PlantRAD52Homologs and Characterization of theArabidopsis thaliana RAD52-Like Genes

et al. 2011

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“…We propose that double-strand breaks are central to all these processes. When double-strand breaks occur, they can be repaired in a number of ways (Aguilera, 2001;Bleuyard et al, 2006;Preston et al, 2006). One possibility is nonhomologous end joining (NHEJ), resulting in chimeric genes.…”

Section: The Sss Process Appears To Be Associated With Nuclear Regulamentioning

confidence: 99%

Plant Mitochondrial Recombination Surveillance Requires Unusual RecA and MutS Homologs

et al. 2007

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For >20 years, the enigmatic behavior of plant mitochondrial genomes has been well described but not well understood. Chimeric genes appear, and occasionally are differentially replicated or expressed, with significant effects on plant phenotype, most notably on male fertility, yet the mechanisms of DNA replication, chimera formation, and recombination have remained elusive. Using mutations in two important genes of mitochondrial DNA metabolism, we have observed reproducible asymmetric recombination events occurring at specific locations in the mitochondrial genome. Based on these experiments and existing models of double-strand break repair, we propose a model for plant mitochondrial DNA replication, chimeric gene formation, and the illegitimate recombination events that lead to stoichiometric changes. We also address the physiological and developmental effects of aberrant events in mitochondrial genome maintenance, showing that mitochondrial genome rearrangements, when controlled, influence plant reproduction, but when uncontrolled, lead to aberrant growth phenotypes and dramatic reduction of the cell cycle.

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“…Cells can repair DSBs by the relatively inaccurate process of rejoining the two broken ends directly (i.e. nonhomologous end joining) or much more accurately by HR (Bleuyard et al, 2006;Wyman and Kanaar, 2006). These two pathways appear to compete for DSBs, but the balance between them differs widely among species, between different cell types of a single species, and during different cell cycle phases of a single cell type (Shrivastav et al, 2008).…”

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confidence: 99%

“…DNA double-strand break (DSB) repair is particularly important in maintaining the integrity of genome among individuals and shuffling genetic information among population, because DSBs are generated not only in meiotic cells but also from the action of certain endogenous or exogenous DNA-damaging agents and during repair of other kinds of DNA lesions by NER or BER (West et al, 2004;Bleuyard et al, 2006). The past decade has witnessed an explosion in understanding of this complex process by using yeast (Saccharomyces cerevisiae) as a model organism (Aylon and Kupiec, 2004).…”

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confidence: 99%

Replication Protein A (RPA1a) Is Required for Meiotic and Somatic DNA Repair But Is Dispensable for DNA Replication and Homologous Recombination in Rice

et al. 2009

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Replication protein A (RPA), a highly conserved single-stranded DNA-binding protein in eukaryotes, is a stable complex comprising three subunits termed RPA1, RPA2, and RPA3. RPA is required for multiple processes in DNA metabolism such as replication, repair, and homologous recombination in yeast (Saccharomyces cerevisiae) and human. Most eukaryotic organisms, including fungi, insects, and vertebrates, have only a single RPA gene that encodes each RPA subunit. Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), however, possess multiple copies of an RPA gene. Rice has three paralogs each of RPA1 and RPA2, and one for RPA3. Previous studies have established their biochemical interactions in vitro and in vivo, but little is known about their exact function in rice. We examined the function of OsRPA1a in rice using a T-DNA insertional mutant. The osrpa1a mutants had a normal phenotype during vegetative growth but were sterile at the reproductive stage. Cytological examination confirmed that no embryo sac formed in female meiocytes and that abnormal chromosomal fragmentation occurred in male meiocytes after anaphase I. Compared with wild type, the osrpa1a mutant showed no visible defects in mitosis and chromosome pairing and synapsis during meiosis. In addition, the osrpa1a mutant was hypersensitive to ultraviolet-C irradiation and the DNA-damaging agents mitomycin C and methyl methanesulfonate. Thus, our data suggest that OsRPA1a plays an essential role in DNA repair but may not participate in, or at least is dispensable for, DNA replication and homologous recombination in rice.

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Recent advances in understanding of the DNA double-strand break repair machinery of plants

Cited by 94 publications

References 143 publications

Identification of PlantRAD52Homologs and Characterization of theArabidopsis thaliana RAD52-Like Genes

Identification of PlantRAD52Homologs and Characterization of theArabidopsis thaliana RAD52-Like Genes

Plant Mitochondrial Recombination Surveillance Requires Unusual RecA and MutS Homologs

Replication Protein A (RPA1a) Is Required for Meiotic and Somatic DNA Repair But Is Dispensable for DNA Replication and Homologous Recombination in Rice

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