Recent advances in understanding basophil functions in vivo

Voehringer, David

doi:10.12688/f1000research.11697.1

Cited by 32 publications

(29 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, the role of basophils during primary infection with helminths appears to be limited (6). Despite previous studies using depleting Abs that suggested a role of basophils during Th2 T cell priming, Th2 T cell expansion was not affected in (22).…”

Section: Discussionmentioning

confidence: 94%

“…The role of basophils during helminth infection has been studied using basophil-deficient mice or basophil-depleting Abs (5). Thus, most studies focused on the role of basophils in gastrointestinal nematode infection (6). In this context, we have shown that basophils are dispensable for the control of the tissue-migrating Strongyloides ratti L3 during primary infection and contribute to the control of parasitic adults in the intestine at the peak of infection (7).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Basophils Are Dispensable for the Control of a Filarial Infection

et al. 2018

Self Cite

View full text Add to dashboard Cite

Basophils are innate effector cells that contribute to allergic reactions and provide protection against parasites. Using basophil-deficient Mcpt8-cre mice, we have previously shown that these granulocytes contributed to the immune mediated early control of the gastrointestinal helminth Strongyloides ratti in mice. In this study, we analyze the impact of basophils on the immune response and defense against the tissue-dwelling filarial helminth parasite Litomosoides sigmodontis. Although basophils and IgE increased at the site of infection, the absence of basophils did not change the outcome of L. sigmodontis infection. Worm burden in the thoracic cavity and microfilaremiae in the peripheral blood were alike in L. sigmodontis–infected Mcpt8-cre mice compared with Mcpt8 wild type littermates during the entire course of infection. Analysis of the cytokine and Ab response to L. sigmodontis revealed no consistent alterations in the absence of basophils. Furthermore, basophil-deficient and -competent mice were protected to the same extent during a secondary infection with L. sigmodontis. In summary, our findings suggest that basophils are dispensable for the initiation of the appropriate immune response and host defense against L. sigmodontis infection in mice.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 98%

Basophils Are Dispensable for the Control of a Filarial Infection

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…A higher interleukin-6/10 ratio has been associated with increased risk of recurrence of BCG-treated patients, while another study found that IL-2 urine levels showed an earlier peak than IL-10 levels in responders vs. non responders. Basophils may have a role in BCG-treated bladder cancer patients because of their ability to facilitate Th2 polarization by secreting IL-4 and skewing antigen-presenting cells towards a type 2 response [17]. In fact, studies in mouse models have shown that depletion of basophils can impair Th2 polarization [18] and that Th2 responses to cysteine protease are mediated by a complex interplay between migratory dermal dendritic cells (DCs) and basophils positive for interleukin 4 (IL-4) via ROS-mediated signaling [19].…”

Section: Discussionmentioning

confidence: 99%

Absolute basophil count is associated with time to recurrence in patients with high-grade T1 bladder cancer receiving bacillus Calmette–Guérin after transurethral resection of the bladder tumor

et al. 2019

View full text Add to dashboard Cite

Background Basophils, eosinophils and monocytes may be involved in BCG-induced immune responses and be associated with outcomes of bladder cancer patients receiving intravesical BCG. Our objective was to explore the association of baseline counts of basophils, eosinophils and monocytes with outcomes of patients with high-grade T1 bladder cancer receiving a standard course of intravesical BCG. Methods We retrospectively reviewed medical records of patients with primary T1 HG/G3 bladder cancer. After re-TURBT, patients were treated with a 6-week course of intravesical BCG induction followed by intravesical BCG every week for 3 weeks given at 3, 6, 12, 18, 24, 30 and 36 months from initiation of therapy The analysis of potential risk factors for recurrence, muscle invasion and cancer-specific and overall survival was performed using univariable Cox regression models. Those factors that presented, at univariate analysis, an association with the event at a liberal p < 0.1, have been selected for the development of a multivariable model. Results A total of 1045 patients with primary T1 HG/G3 were included. A total of 678 (64.9%) recurrences, 303 (29.0%) progressions and 150 (14.3%) deaths were observed during follow-up. Multivariate analysis showed that logarithmic transformation of basophils count was associated with a 30% increment in the hazard of recurrence per unit increase of logarithmic basophils count (HR 1.30; 95% confidence interval 1.09-1.54; p = 0.0026). Basophil count modeled by quartiles was also significantly associated with time to recurrence [second vs. lower quartile HR 1.42 (1.12-1.79); p = 0.003, third vs. lower quartile HR 1.26 (1.01-1.57); p = 0.041; upper vs. lower quartile HR 1.36 (1.1-1.68); p = 0.005]. The limitations of a retrospective study are applicable. Conclusion Baseline basophil count may predict recurrence in BCG-treated HG/G3 T1 bladder cancer patients. External validation is warranted.

show abstract

“…Owing to their phenotypic similarity with mast cells and their rarity, basophils had often erroneously been considered as blood-circulating precursors of tissue-resident mast cells or minor and possibly redundant relatives of mast cells, and therefore neglected in immunological studies ( 29 ). Recent development of tools useful for functional analysis, including genetically-engineered mice deficient only in basophils ( 30 – 36 ) ( Figure 1 ), has successfully illustrated the nonredundant roles of basophils, distinct from those played by mast cells, in a series of immune responses, including protective immunity to parasitic infections, allergic inflammation, autoimmune diseases, and regulation of innate and acquired immunity ( 37 – 39 ). In this article, we focus on the cellular and molecular mechanisms underlying ATR that have been clarified in animal models of tick infestation.…”

Section: Introductionmentioning

confidence: 99%

Immunobiology of Acquired Resistance to Ticks

2020

View full text Add to dashboard Cite

Ticks are blood-sucking arthropods of great importance in the medical and veterinary fields worldwide. They are considered second only to mosquitos as vectors of pathogenic microorganisms that can cause serious infectious disorders, such as Lyme borreliosis and tick-borne encephalitis. Hard ( Ixodid ) ticks feed on host animals for several days and inject saliva together with pathogens to hosts during blood feeding. Some animal species can acquire resistance to blood-feeding by ticks after a single or repeated tick infestation, resulting in decreased weights and numbers of engorged ticks or the death of ticks in subsequent infestations. Importantly, this acquired tick resistance (ATR) can reduce the risk of pathogen transmission from pathogen-infected ticks to hosts. This is the basis for the development of tick antigen-targeted vaccines to forestall tick infestation and tick-borne diseases. Accumulation of basophils is detected in the tick re-infested skin lesion of animals showing ATR, and the ablation of basophils abolishes ATR in mice and guinea pigs, illustrating the critical role for basophils in the expression of ATR. In this review article, we provide a comprehensive overview of recent advances in our understanding of the cellular and molecular mechanisms responsible for the development and manifestation of ATR, with a particular focus on the role of basophils.

show abstract

Recent advances in understanding basophil functions in vivo

Cited by 32 publications

References 52 publications

Basophils Are Dispensable for the Control of a Filarial Infection

Basophils Are Dispensable for the Control of a Filarial Infection

Absolute basophil count is associated with time to recurrence in patients with high-grade T1 bladder cancer receiving bacillus Calmette–Guérin after transurethral resection of the bladder tumor

Immunobiology of Acquired Resistance to Ticks

Contact Info

Product

Resources

About