Recent advances in ultrasound-controlled fluorescence technology for deep tissue optical imaging

Screening, Construction, and Preliminary Evaluation of CLDN18.2-Specific Peptides for Noninvasive Molecular Imaging

“…In this study, 293T CLDN18.1 cells were selected as negative uptake cells for incubation with the phage display peptide library to preferentially remove CLDN18.1-specific peptides, followed by incubation with 293T CLDN18.2 cells to obtain CLDN18.2-specific peptides. Furthermore, the use of whole-cell screening systems is more likely to preserve the biological structure and function of the target protein than the commonly used protein-coated solid-phase screening model. − Fluorescent labeling is an important strategy in drug research to assess the specificity of drugs to target cells and the site of binding, FITC-T37 exhibited CLDN18.2 specificity at the cellular level and, more importantly, specifically recognized and bound CLDN18.2-positive cell lines but not CLDN18.1-positive cell lines.…”

Advances in the design and use of carbon dots for analytical and biomedical applications

“…The entry mechanism of CDs will vary with size, surface charge, and surface functionalization, as well as the cell type and their microenvironment (figure 1). Their distinct optical features and simple functionalization make them ideal for deep-tissue fluorescence imaging as they can be tuned for excitation and emission in far-red wavelengths [22].…”