Recent Advances in Tuberculosis Diagnosis: IGRAs and Molecular Biology

Pyrosequencing for rapid detection of Mycobacterium tuberculosis second-line drugs and ethambutol resistance

“…Resistance to FLQ is associated with mutations in the quinolone resistance determining region (QRDR) in gyrA, and to a lesser extent in gyrB. The reference molecular method to detect these mutations is sequencing, but different methods based on reverse hybridization [12][13][14], real-time PCR or pyrosequencing [15] have also been developed [16][17][18][19][20]. In a previous work, we In Table 3 are presented the mutations detected in the different loci analyzed.…”

Evaluation of GenoFlow DR-MTB Array Test for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis

“…Nowadays, several molecular tests are available (18)(19)(20)(21), but more studies are still needed to assess their clinical value. For instance, an evaluation has demonstrated the noninferiority of the GenoType MTBDRplus version 2.0 and Nipro line probe assays in comparison to the WHO-endorsed first version of the GenoType MTBDRplus assay for the rapid detection of multidrug-resistant tuberculosis (MDR-TB) (22).…”

Molecular Characterization of Mycobacterium tuberculosis Strains with TB-SPRINT

“…1 Because of the slow growth rate of M. tuberculosis, phenotypic drug susceptibility testing (DST) takes several weeks, and different molecular methods have been developed for rapid detection of mutations associated with drug resistance. 5 The objective of this study was to evaluate a molecular method based on multiplex PCR and hybridization on microbeads for simultaneous spoligotyping and detection of mutations associated with rifampicin (RIF) and isoniazid (INH) resistance, termed Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT, Beamedex SAS, Orsay, France). 6 A total of 67 M. tuberculosis complex strains isolated in Spain were retrospectively selected from the collection in Instituto de Investigación Sanitaria de Aragón, Zaragoza, Spain.…”