Recent Advances in the Utilization of Brown Macroalgae as Feedstock for Microbial Biorefinery

Woo, Sunghwa; Moon, Jo Hyun; Sung, Junyeong; Baek, Dongyeop; Shon, Yang Jun; Jung, Gyoo Yeol

doi:10.1007/s12257-022-0301-8

Cited by 9 publications

(6 citation statements)

References 132 publications

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“…Under the proposed optimal conditions, mannitol achieved 78% of the theoretical maximum yield (i.e., 267 g/kg biomass), confirming the potential of S. japonica as a feedstock for mannitol recovery. It is expected that the recovered mannitol can be utilized as a raw material to produce various bioproducts such as lactic acid, microbial enzymes, and microbial exopolysaccharide (Woo et al., 2022; Yang et al., 2019; Yoo et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Fermentable carbohydrates recovered from brown algal biomass can be used as raw materials for microbial fermentation and then converted into bioproducts and biofuels such as lactic acid, itaconic acid, and bioethanol (Woo et al, 2022). Lactic acid and its derivatives are important biomaterials with a wide range of applications in bioplastic, food, and cosmetic industries (Kang & Kwak, 2024;Liu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Improved recovery of mannitol from Saccharina japonica under optimal hot water extraction and application to lactic acid production by Lacticaseibacillus rhamnosus

Lee,

Bae,

Shin

et al. 2024

GCB Bioenergy

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Brown algae are gaining traction as biorefinery feedstocks due to their advantages such as rapid growth and carbon dioxide sequestration. Saccharina japonica has high potential due to its high carbohydrate content, especially mannitol (26.7%). In this study, a biorefinery process for S. japonica was designed, with focusing on sugar conversion and bioconversion into lactic acid, a valuable platform chemical utilized in various industries. The existing sugar conversion process of S. japonica has been investigated by focusing on enzymatic or acid‐catalyzed hydrolysis, but not hot water extraction although mannitol can be easily recovered using water. The effect of temperature (60–120°C) on the mannitol yield from S. japonica was investigated, and a mannitol yield of 208 g/kg biomass was achieved at the optimal temperature of 100°C (about 78% of the theoretical maximum yield). This study emphasizes that this simple process has considerable potential for application as over 80% of the fermentable carbohydrates in S. japonica were mannitol. Then, S. japonica extract was applied to lactic acid production. First, lactic acid production of four bacterial strains was tested in a mannitol medium, and Lacticaseibacillus rhamnosus was selected as the superior producer, showing 1.93 to 2.92 times better lactic acid titer than others. Next, the optimal feeding concentration of mannitol was determined to be 20 g/L, which was all consumed by L. rhamnosus. Finally, S. japonica extract was applied to lactic acid production by L. rhamnosus, and the results showed similar fermentation profiles with the control medium: lactic acid production, 18.81 g/L (control: 18.97 g/L); lactic acid conversion, 95.1% (control: 95.9%); cell growth (OD600 nm), 8.9 (control: 7.4). The lactic acid yield in the designed biorefinery process was estimated to be 195.6 g/kg biomass, thus S. japonica has high potential as a biorefinery feedstock to produce valuable bioproducts, including lactic acid.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved recovery of mannitol from Saccharina japonica under optimal hot water extraction and application to lactic acid production by Lacticaseibacillus rhamnosus

Lee,

Bae,

Shin

et al. 2024

GCB Bioenergy

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“…The biological production of itaconate generally entails glucose metabolism by cis -aconitate decarboxylase (Cad) from Aspergillus terreus (A. terreus). − However, because starch crops are the primary source of glucose, a significant increase in itaconate production via this route is problematic, as starch crops are also utilized as a food resource and their cost fluctuates depending on climate and global issues. , To overcome these issues, several alternative substrates are currently being investigated. These include xylose, galactose, and acetate, which are derived from terrestrial plants. − …”

Section: Introductionmentioning

confidence: 99%

“…Brown macroalgae are not utilized as a food resource, with the exception of a few countries. More importantly, these seaweeds display many characteristics of an ideal feedstock, such as high carbohydrate content and biomass productivity. ,,− Specifically, the carbohydrate content of Saccharina japonica (S. japonica), the most commonly cultivated seaweed, reaches 67%, comprising varying ratios of alginate and mannitol depending on the harvest season. ,, Alginate is a copolymer of homopolymeric blocks of β-D-mannuronate and α-L-guluronate.…”

Section: Introductionmentioning

confidence: 99%

Direct Itaconate Production from Brown Macroalgae Using Engineered Vibrio sp. dhg

Moon,

Woo,

Shin

et al. 2024

J. Agric. Food Chem.

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Itaconate is a promising platform chemical with broad applicability, including the synthesis of poly(methyl methacrylate). Most studies on microbial itaconate production entail the use of crop-based feedstock, which imposes constraints due to its limited supply. Brown macroalgae have recently gained attention as next-generation biomass owing to their high biomass productivity and carbohydrate content and amenability to mass production. Therefore, the use of macroalgae for itaconate production warrants exploration. In this study, the direct production of itaconate from brown macroalgae was demonstrated using engineered Vibrio sp. dhg, which has emerged as an efficient platform host for brown macroalgal biorefineries. Specifically, to enhance production, cis-aconitate decarboxylase (Cad) from Aspergillus terreus was heterologously expressed and isocitrate dehydrogenase (icd) was deleted. Notably, the resulting strain, VIC, achieved itaconate titers of 2.5 and 1.5 g/L from a mixture of alginate and mannitol (10 g/L of each) and 40 g/L of raw Saccharina japonica (S. japonica), respectively. Overall, this study highlights the utility of brown macroalgae as feedstock, as well as that of Vibrio sp. dhg as a platform strain for improving itaconate bioproduction.

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“…According to integrated or multi-step biorefineries, an extraction process is generally chosen as a first step because valuable components (e.g., carbohydrates) still remain in the residual biomass after the extraction, which can be further valorized through additional processes such as hydrothermal liquefaction and pyrolysis (Guo et al, 2019;Kim et al, 2021;Loṕez-Linares et al, 2021;de Almeida-Couto et al, 2022). Among various marine resources, macroalgae sequester carbon dioxide as primary producers, have high value as a sustainable resource due to their fast growth, and contain high content of carbohydrates and bioactive substances, thus are suitable for the described biorefinery feedstock (Choi, 2019;Lim et al, 2019;Woo et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of dieckol extraction yield from Ecklonia cava through optimization of major variables in generally recognized as safe solvent-based process

Shin,

Lee,

Bae

et al. 2023

Front. Mar. Sci.

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Ecklonia cava (EC), an edible brown macroalga abundant in intertidal areas of East Asia (Korea, Japan, and China), contains high-value bioactive compounds such as dieckol, which has antifungal, anti-inflammatory, antitumor, and antihyperlipidemic activities. However, no studies have been reported on the utilization of EC as a biorefinery feedstock, and the design of a more economical and high-yield process is required for the utilization of dieckol for the human healthcare industry. In this study, we designed a bioprocess for the high-yield recovery of dieckol from EC in a generally recognized as safe (GRAS) solvent to facilitate its application in the food and healthcare industries. Preliminary studies identified ethanol as an efficient solvent with the highest dieckol extraction yield (2.9 mg/g biomass). In order to maximize the recovery of dieckol from EC, the major extraction variables (solvent concentration, reaction temperature, and reaction time) were optimized based on statistical methods. Based on the predictive model, the numerical optimization determined that the solution with the highest dieckol content per weight of extract (62.6 vol% ethanol concentration, 54.2°C temperature, 13.2 min) was the optimal extraction condition. Under the determined conditions, the dieckol yield from EC achieved 6.4 mg dieckol/g EC (95.5% agreement with the predicted value). The designed process offers several advantages, including improving the utilization feasibility of EC, utilizing GRAS solvents with potential human applications, short extraction time (13.2 min), maximized process yield, and the highest dieckol recovery compared to previous reports.

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Recent Advances in the Utilization of Brown Macroalgae as Feedstock for Microbial Biorefinery

Cited by 9 publications

References 132 publications

Improved recovery of mannitol from Saccharina japonica under optimal hot water extraction and application to lactic acid production by Lacticaseibacillus rhamnosus

Improved recovery of mannitol from Saccharina japonica under optimal hot water extraction and application to lactic acid production by Lacticaseibacillus rhamnosus

Direct Itaconate Production from Brown Macroalgae Using Engineered Vibrio sp. dhg

Enhancement of dieckol extraction yield from Ecklonia cava through optimization of major variables in generally recognized as safe solvent-based process

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